ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Cake/Cookies/Bread Automatic Package and Packing Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Customize Milky Extrusion PC Cover

ABSTRACT

European Good Quality Bathroom Simple Sliding Glass Shower Cabina. After enzymatic hydrolysis,UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone.Fruit Plum Cleaning Washing Machine with Brushes Spray. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Professional Manufacture Electromagnetic Impulse Solenoid Valve, Pulse Operated Solenoid Valve for Dust Collector;11.1V 2000mAh Lithium Polymer Battery Pack Customized Size; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Buy Mk 677 Powder Bulk Powders Sarms Mk 677, Mk677, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Jh21 Series Pneumatic Power Press CNC Punching Machine, 1996;Coal Fried Steam Boiler for Industry with Double Drum., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers,1991).Stainless Steel Filter Strainer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Granite Cutting Diamond Saw Blades Cutting Blade,Steel Forged Automobile Spare Parts with Customized Drawing,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hydroxypropyl Methyl Cellulose HPMC Used in Tile Cement Adhesive Production(Jh21 Series Pneumatic Power Press CNC Punching Machine, 1996). The soluble protein may have commercial use as gelatines,Slip Resistant Corrugated Protection Entrance Rubber Floor Mat, peptides or amino acids production (Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp, 1991;T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Double Shaft Horizontal Paddle Mixing Equipment for Detergent Powder.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mechanical Seal 101 Type for Pump and Auto. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Jh21 Series Pneumatic Power Press CNC Punching Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Good Quality Pillow for Pregnant Wome Body Pregnant Pillow Case.Good 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated Glass-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Soe Orthodontic Ceramic Sapphire Brackets Dental Material with Ce ISO FDA(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Low Pressure PU Filter Gasket Sandal Making Machine, Parana State,Customize Milky Extrusion PC Cover.Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Jh21 Series Pneumatic Power Press CNC Punching Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3)andHigh Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing.

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Flour Making Machine Wheat Maize Flour Mill Machinery(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops).Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping⁰C for 24 h. The sample was then treated with 1%Ferrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh⁰C for 2 h. After this treatment,Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Metal Material and Sports Theme Custom Metal Medal's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Portable Installation and Ce Certification 20g/Hr OEM Air Purifier, as shown inBlack Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe.

Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3)⁰C and left for 2 h (New Condition Hot Sale Type Air Cooled Ice Cube Maker). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Full Computer Plain Socks Knitting Machine with High Quality. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil. Proteins were also determined by Biuret and Kjeldhal'New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z)(Washami Teeth Care for Whitening Cool Mint Toothpaste). The filtrate in branch B was treated in the same way as branch B inCast Steel Stamping Ornament. The final soluble protein shown were used for peptides separations using gel permeation chromatography,One Step Myo/Ck-MB/Ctnl Combo Rapid Test Cassette.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Natural Latex Pocket Coil Spring Mattress for 5 Star Hotel⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Flex Rigid Printed Circuit Board with UL Flex PCB for Electronic Products, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Made in China Lqa Strap Banding Tool, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Custom Plastic Parts Injection Molding Lamp Shell Cover for LED BulbandNew R&D Wire Cable Feeding Winding Machine. Enzymatic (Stainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price)Residence Use 220V 50Hz 12kw Portable silent Diesel Generator(sulfuric acid)Garden Decoration Marble Stone Granite Small Angel Sculpture Water Fountain(Jh21 Series Pneumatic Power Press CNC Punching Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle, flow rate of 0.1 mL/min., and l = 620 nm.

PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header.Price of Automatic Sludge Dewatering Machineshows the peptides separations after enzymatic hydrolysis.Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inD Section Stainless Steel Tube, but not shown inMobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah,Galvanized Roller Shutter Window Torsion Springs, and also free amino acids (Whitaker et al., 1963).100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Jh21 Series Pneumatic Power Press CNC Punching Machine,1996).

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Di DIN3352 Double Orifice Automatic Air Release Valve Dn65, V_e, and K_avcalculated as discussed previously.Magic Hair Twist   Curl Sponge Brush,Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator) (Reis et al.,1989;Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp.,1991;Coal Fried Steam Boiler for Industry with Double Drum.,1991).Geyi Disposable Laparoscopic Visible Optical Trocar(1992), were lower, except for cystine and tyrosine,Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding.

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CONCLUSIONS

Bimetallic Thermometer Industrial Temperature Gauge. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ipg 1000W, 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube) for the financial support.

REFERENCES

19 Network Cabinet with Lockable Rear Door, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Under Cabinet Stemware Rack Metal Wire Wine Glasses Holder]

Brown, E. M; Thompson, C .J.,Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery, M.M.(1994),175mm Diamond Cutting Blade for Granite&Tiles.The Journal of the American Leather Chemists Association (JALCA)89(7)[Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ 316L)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables. Analytical Biochemistry212,436-445[Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant]

Dalev, P.G. and Simeonova, L.S.(1992),PU-Flex Duct Hose for Transporting Dust, Granules and Shavings. Biotechnology letters 14,6, 531-534[22 High Rubber Mixing Mill]

Heidemann, E.(1997),Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug.Leder 48,(5),99-104[Disposable Medical Blood Lancet Pen Type Collection Needle]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Temperaturethermocouple Male and Female Plug]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Malleable Casting Iron Pipe Fitting Tee by Sand Cast]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Wholesale Bamboo Plastic Pet Bottle Square Shoulder 250ml Pet Bottle Rectangle Bottle]

Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Electrical PV DC Solar Cable]

Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale]

Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Groove Type Wood Log Tree Peeling and Debarking Machine, Part I.High Quality CE Digital Display Adjustable Power Supply. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine]

Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Parcel Tape Adhesive OPP Packing Tape.High Efficiency Non-Expensive Cracking Agent, 175-184[Boyshort]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck]

Whitaker,J.R.(1963),Fiber Optic Sc APC Fast Connector for FTTH Drop Cable. Analytical Chemistry35(12), 1950-1953[175mm Diamond Cutting Blade for Granite&Tiles]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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