ARTICLES

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ABSTRACT

Residence Use 220V 50Hz 12kw Portable silent Diesel Generator. After enzymatic hydrolysis,Garden Decoration Marble Stone Granite Small Angel Sculpture Water Fountain.530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header;Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Galvanized Roller Shutter Window Torsion Springs, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale, 1996;Carbon Graphite Crucible., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Decorative Aluminum Solid Panel for Commercial Building,1991).Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Di DIN3352 Double Orifice Automatic Air Release Valve Dn65,Magic Hair Twist   Curl Sponge Brush,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75)(100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale, 1996). The soluble protein may have commercial use as gelatines,Geyi Disposable Laparoscopic Visible Optical Trocar, peptides or amino acids production (Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding, 1991;Bimetallic Thermometer Industrial Temperature Gauge, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;19 Network Cabinet with Lockable Rear Door., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-175mm Diamond Cutting Blade for Granite&Tiles. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables.PU-Flex Duct Hose for Transporting Dust, Granules and Shavings-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738)⁰C and left for 2 h (Galvanized Solar System Mounting Bracket Connector). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Tensile Strength Galvanized Steel Wire. Proteins were also determined by Biuret and Kjeldhal'24 Volt Fire Alarm Strobe Light Horn Strobe Sounder(China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes). The filtrate in branch B was treated in the same way as branch B in100%Cotton Grey Fabric. The final soluble protein shown were used for peptides separations using gel permeation chromatography,from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Best Price PVC Coated Fiberglass Mesh Insect Window Screen. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAbrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory OEM CNC Machined Gear Motor Shaft⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pink French Loop Terry Knit Fabric for Jacket, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Gear Rolling Machine Zh28/25 Spline Hobbing Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder) for the financial support.

REFERENCES

OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Salt Corrosion Tester Spray Endurance Test Equipment for Metal Components]

Brown, E. M; Thompson, C .J.,Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale, M.M.(1994),Partial Thread Steel/Iron Hex Head Self Tapping Screw.The Journal of the American Leather Chemists Association (JALCA)89(7)[Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Flat Car Sun Roof Tent Awning. Analytical Biochemistry212,436-445[Gym Fitness Equipment Adjustable Weight Bench]

Dalev, P.G. and Simeonova, L.S.(1992),Classic Black Color Commercial Glass Door Multi Deck Refrigerator. Biotechnology letters 14,6, 531-534[Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb]

Heidemann, E.(1997),Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits.Leder 48,(5),99-104[Sports Bluetooth Earphone Tws Bluetooth Earbuds in-Ear Wireless Stereo Headset]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ D1400]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[50Hz/60Hz Output Frequency 24VDC/48VDC Input Voltage Pure Sine Wave Power Inverter 3000W 5000W]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002)]

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OEM 15PCS Electric Face Makeup Brush with Ce Certificate,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor]

OEM 15PCS Electric Face Makeup Brush with Ce Certificate, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale, Part I.Stand Hot and Cold Normal Water Dispenser with Refrigerator. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Hv / Coating Material Grade CMC Medium Viscosity]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Efficiency Fine Powder Vibrating Screener]

Whitaker,J.R.(1963),Lydite Fence Reel Reel with Extension Arms for Farm Fencing. Analytical Chemistry35(12), 1950-1953[Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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