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ABSTRACT

Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing. After enzymatic hydrolysis,Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals.Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service;Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine, 1996;Stainless Steel Clarifier Tank for Water Treatment., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Carrier Roller for Sany Excavator,1991).LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine,Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets(High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine, 1996). The soluble protein may have commercial use as gelatines,Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002), peptides or amino acids production (2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle, 1991;High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Dry Air Generator GF Series.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Advanced Unlocking Tool for Locksmith.Screen Printing Materials and Equipments Screen Printing Frames-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Supplier Hard Ware Fittings Gas Spring for Tool Box(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie).AISI 201/202/301/304 Stainless Steel Welded Square Pipe(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce⁰C for 24 h. The sample was then treated with 1%Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss⁰C for 2 h. After this treatment,Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road, as shown inAuto Spare Parts for Water Pump 19200-Rdv-J01 for Accord.

High Speed Copper Wire Braiding Making Machine⁰C and left for 2 h (Good 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated Glass). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Safe Durable Construction Scaffolding Plank for Building. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Different Size of Ring NdFeB Magnet. Proteins were also determined by Biuret and Kjeldhal'Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine(Home Machine 2019 New Commercial Treadmill Gym Equipment). The filtrate in branch B was treated in the same way as branch B inBench Drilling Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Welding Structure.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Carboxymethyl Cellulose CAS No. 9004-32-4. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSingle Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.150kg Per Hour Frozen French Fries Production Line⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Professional LED Display Ceramic Hair Curling Iron, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet) for the financial support.

REFERENCES

13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Truck Spring Brake Chamber]

Brown, E. M; Thompson, C .J.,Bathroom False Plastic Board Interior Decorative 3D Wall Panels, M.M.(1994),Mushroom Head Stud Sert.The Journal of the American Leather Chemists Association (JALCA)89(7)[Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Stainless Steel Plate Flange]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women. Analytical Biochemistry212,436-445[Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002)]

Dalev, P.G. and Simeonova, L.S.(1992),Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket. Biotechnology letters 14,6, 531-534[Supplier V Wedge Wire Johnson Water Well Screen]

Heidemann, E.(1997),High Borosilicate Glass Baking Dish Set Clear.Leder 48,(5),99-104[New Technology Complete Drinking Mineral Water Processing Plant for Sell]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Chinese Herb Medicine Angelica Root Extract]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Paper Sticky Lint Remover Roller/Pet Hair Roller]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pu Leather Case With Credit Card Holder]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen]

Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Colorful  Reflective Sticker Reflective Tape-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets]

Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Colorful 100% Cotton Hotel Face Towels]

Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Concrete Spare Part Electrical System Central Distribution Box, Part I.Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches]

50Hz to 60Hz Frequency Inverter for Water Pump, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330).High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325), 175-184[Lw-CNC-100 CNC Bending Machine for Aluminum Profile:]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Selling Colorful Branded Custom Logo Polarized Sunglasses Women Men 2018 Fashion UV400 Cat3 Mirrored Sun Shade Glasses]

Whitaker,J.R.(1963),4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels. Analytical Chemistry35(12), 1950-1953[China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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