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ABSTRACT

100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears. After enzymatic hydrolysis,Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole.60ml 100ml Customized Glass Jar for Spice, Candle, Candy. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Outlet Automatic Grain Tower Dryer Drying Equipment, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mesh Bag Packing 20kg Pure White Garlic, 1996;Supreme 524 Mv Sea Kayak., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Trunk Organizer Foldable Water Proof,1991).100%Polyester Burnout Velvet Sofa Fabric (pineapple grid). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Diesel Generator Parts AVR Ki-Davr-95s for Kipor,Gld Brand Padlock Brass Popular in 5 Continents  ,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,No Lead Bronze Flange Manufacturer(Mesh Bag Packing 20kg Pure White Garlic, 1996). The soluble protein may have commercial use as gelatines,Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube, peptides or amino acids production (Snsc Fd30 Side Shift New Diesel Forklift 3ton, 1991;Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Laptop Bag College Men Backpack Leather Back Pack Bags.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pet Dog Grooming Rake Dematting Tool Shedding Comb. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mesh Bag Packing 20kg Pure White Garlic, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom Apparel Applique Round Patch School Club Embroidery Collar Patch.Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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White Cotton Satin Hotel Duvet Cover (DPFB8055)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory Wholesale Zinc Plated Carbon Steel Hex Nut).Small Cleaning Brush ESD Anti Static Brush ESD Brush Black(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PAC /Poly Aluminium Chloride for Water Treatment⁰C for 24 h. The sample was then treated with 1%Aluminum Sheet Shearing Machine⁰C for 2 h. After this treatment,Electrical PV DC Solar Cable. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Tomato Bubble and High Pressure Washing Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Upholstery Ribbon Christmas Tape, as shown inOEM Double Sided Rigid PCB Circuit Board Manufacturer.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Factory Supplied High Strength Marine Anchor Chain. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHumidity Refrigeration Digital Controller Jsd-100+(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Modern 4 Person Workstation with Computer Desk Modular Office Furniture, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Wooden Grain Perforated Panel for Metal Mesh Curtain Wall. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Multi-Functional Backpack Manual 52cc Brush Cutter) for the financial support.

REFERENCES

Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Middle Island/Height Adjustable Metal Clothing Display Gondola Stand for Shop]

Brown, E. M; Thompson, C .J.,Folding  Platform Hand  Trolley , M.M.(1994),Pump Flower Sprinkler with Automatic Watering System.The Journal of the American Leather Chemists Association (JALCA)89(7)[European Good Quality Bathroom Simple Sliding Glass Shower Cabina]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Quality Horizontal Flat Bed Metal CNC Turning Lathe Machine Price Ck6163]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant. Analytical Biochemistry212,436-445[2019 Foam Concrete Machine/Foamed Concrete Block Making Machine]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Silver 8 Inch Double Thick Round Cake Board]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ODU 14 Pin Industrial Circular Connector]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Permanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Uhp Graphite Electrode With Nipple]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone]

Whitaker,J.R.(1963),High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier. Analytical Chemistry35(12), 1950-1953[Cake/Cookies/Bread Automatic Package and Packing Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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