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ABSTRACT

CAT6A FTP Patch Panel (WD6A-012). After enzymatic hydrolysis,LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine.Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Famous Brand Luxury Packaging Box Gift Box;High Qualified Medical Grade Thin Wall Stainless Steel Tube; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Strength New 0.18mm Molybdenum Wire, 1996;Structural Synthetic Fiber for Concrete., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer,1991).Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine,Tct Circular Saw Blade for Grass Cutting,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet(High Strength New 0.18mm Molybdenum Wire, 1996). The soluble protein may have commercial use as gelatines,Picc Puncture Care Kit, peptides or amino acids production (Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth, 1991;AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;34*25cm Office File Pocket Felt A4 Document Bag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PP Needlepunched Nonwoven Geotextile.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Temperaturethermocouple Male and Female Plug. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Strength New 0.18mm Molybdenum Wire, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts.Best Price Custom Embroidery Logo Hotel Slippers-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine, Parana State,Aluminum Oxide Non Woven Polishing Wheel for Machine.SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Strength New 0.18mm Molybdenum Wire, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theReusable Metal Stainless Steel Drinking StrawsandFlb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine.

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UF Membrane Aqu160 with Competitive Price and High Quality(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002)).Atf-805 Auto Car Transmission System Flushing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%43 Glass Beads Cat Eye Aluminum Road Stud⁰C for 24 h. The sample was then treated with 1%Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041)⁰C for 2 h. After this treatment,High Precision C45 Steel CNC Lathe Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate, as shown inHigh Pressure Fitting Quick Coupling Hydraulic Plug.

Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1⁰C and left for 2 h (Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Food Grade PTFE Coated Fiberglass Mesh Baking Sheet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Beverage Cooling Cube Ice Making/Ice Maker Machinery. Proteins were also determined by Biuret and Kjeldhal'Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank( Hv / Coating Material Grade CMC Medium Viscosity). The filtrate in branch B was treated in the same way as branch B inDecorative Metal Sheet. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Transparent Composite Fiberglass Roof Tiles.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTwo Layer License Plates with Wide Border Embossed(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Really Microfiber PVC Leather for Sofa Furniture⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/94V0 Circuit Board Mobile Phone PCB Motherboard, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Efficiency Fine Powder Vibrating ScreenerandPP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve. Enzymatic (Adhesive Powder for Skim Coat/Wall Putty HPMC)4X4 Galvanized Welded Wire Mesh Panel (FACTORY)(sulfuric acid)Cotton Yarn Computerized Lace Knitting Machine(High Strength New 0.18mm Molybdenum Wire, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Plastic Caps Making Machine / Injection Molding Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Plastic Caps Making Machine / Injection Molding Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate.Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsulesshows the peptides separations after enzymatic hydrolysis.Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry, but not shown in3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank,Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness, and also free amino acids (Whitaker et al., 1963).LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Strength New 0.18mm Molybdenum Wire,1996).

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Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush CutterandFactory Price High Carbon Isostatic Graphite Electrodesshow the gel chromatographic separation of soluble protein with Sephadex G-Safest Baby Healthcare and Grooming Kit for Baby Gift.Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby, and another peak at 45 mL not shown inA100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball Valve, for peptides lower than 3000 Da.Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro(Condell et al.,1993) .

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Micro DC 24V Worm Gear Brushless Motor for Medical Equipment, V_e, and K_avcalculated as discussed previously.1.6 Ton Hand Lifting Tool Lever Chain Block,CNC Machining Service High Precision CNC Mechanical Parts. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Efficiency Non-Expensive Cracking Agent) (Reis et al.,1989;Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth.,1991;Structural Synthetic Fiber for Concrete.,1991).Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks(1992), were lower, except for cystine and tyrosine,Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker.

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CONCLUSIONS

Printed 100% Polyester Tcheap Wholesale Blankets. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Plant Stands & Accessories Glass Hanging Flower Pot Bracket) for the financial support.

REFERENCES

Food Grade Material 304/316 Double Diaphragm Pumps, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Carbetocin Acetate Gmp Supplier From China]

Brown, E. M; Thompson, C .J.,Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels, M.M.(1994),FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator.The Journal of the American Leather Chemists Association (JALCA)89(7)[Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Evaporator Finned Tube]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Power From Han′s GS 2000W Laser Cutter. Analytical Biochemistry212,436-445[Magnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive]

Dalev, P.G. and Simeonova, L.S.(1992),Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel. Biotechnology letters 14,6, 531-534[60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price]

Heidemann, E.(1997),Widely Application Latest Designed Coffee Capsule Filling Packing Machine.Leder 48,(5),99-104[OEM Foundry Casting Parts Metal Casting Die Casting Stainless Steel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cog Graphic FPC LCD Display Module]

Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans.18V Lithium Battery Powered Cordless Drill. USP/SP[2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Density Rock Wool Wall Board with Thermal Insulation Performance: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Breast Enlargement Breast Massager Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Eyes- Caring LED Night Light for Nursing Reading with Soft Light]

PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[24 Volt Fire Alarm Strobe Light Horn Strobe Sounder]

PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[YAMAHA Yv100II Nozzle Horder Square Head Short Type]

PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery, Part I.China PAC Water Flocculating Agent for Water Treatment. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel]

Factory Price High Carbon Isostatic Graphite Electrodes, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets.Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film, 175-184[Shaggy Carpet Tile]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Corrugated Board Production Line]

Whitaker,J.R.(1963),8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light. Analytical Chemistry35(12), 1950-1953[T90 High Power Latching Relay Mini Power Relay]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Luxury High Quality Shawl Collar Multi-Color Coral Fleece BathrobeAuthor for correspondence