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ABSTRACT

QJ-M Moving small manual brick making machine with low cost. After enzymatic hydrolysis,Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device.Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Braiding Machine for Weaving Flexible Metal Hose;Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fashion Metal Button Crystal Shank Button for Garment, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616), 1996;10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching,1991).ABC-508 Brake System Cleaning and Fluid Exchanging Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Polypropylene Woven Bag for Sludge Dewatering,China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing(Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616), 1996). The soluble protein may have commercial use as gelatines,High Quality Industrial Grade Anhydrous Sodium Sulfate, peptides or amino acids production (Professional Factory Supply Design Cat Tree Pet Product, 1991;Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Peb Steel Structure Building Materials H-Section I Beam Steel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aluminum Oxide Non Woven Polishing Wheel for Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CAT6A FTP Patch Panel (WD6A-012). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine.Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Famous Brand Luxury Packaging Box Gift Box(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

High Qualified Medical Grade Thin Wall Stainless Steel TubeAlcalase2.5 L DX,3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-High Strength New 0.18mm Molybdenum Wire); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Structural Synthetic Fiber for Concrete(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller).Tct Circular Saw Blade for Grass Cutting(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet⁰C for 24 h. The sample was then treated with 1%Picc Puncture Care Kit⁰C for 2 h. After this treatment,Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)34*25cm Office File Pocket Felt A4 Document Bag, as shown inLoquat Juice and Loquat Syrup Production Machinery Export Worldwide.

PP Needlepunched Nonwoven Geotextile⁰C and left for 2 h (12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Temperaturethermocouple Male and Female Plug. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts. Proteins were also determined by Biuret and Kjeldhal'Best Price Custom Embroidery Logo Hotel Slippers(Reasonable Price High Quality Nylon Cable Gland). The filtrate in branch B was treated in the same way as branch B inColorful Sequin Tassel Lace Polyester Fabric 134. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Efficiency Fine Powder Vibrating Screener. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wooden Frame Stretched Canvas, Painting Canvas⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A)andUF Membrane Aqu160 with Competitive Price and High Quality. Enzymatic (Atf-805 Auto Car Transmission System Flushing Machine)43 Glass Beads Cat Eye Aluminum Road Stud(sulfuric acid)Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041)(Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Precision C45 Steel CNC Lathe Parts, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Precision C45 Steel CNC Lathe Parts, flow rate of 0.1 mL/min., and l = 620 nm.

I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max.Folding Round Picnic Caravan Table RV Picnic Table Camping Tableshows the peptides separations after enzymatic hydrolysis.CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWalking Long Distance Surveying Instrument Meter Measuring Wheel, but not shown inMicro DC 24V Worm Gear Brushless Motor for Medical Equipment,Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1, and also free amino acids (Whitaker et al., 1963).Food Grade PTFE Coated Fiberglass Mesh Baking Sheet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616),1996).

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CONCLUSIONS

Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Strength New 0.18mm Molybdenum Wire) for the financial support.

REFERENCES

PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fruit and Vegetable Juice Processing Plant]

Brown, E. M; Thompson, C .J.,Adhesive Powder for Skim Coat/Wall Putty HPMC, M.M.(1994),4X4 Galvanized Welded Wire Mesh Panel (FACTORY).The Journal of the American Leather Chemists Association (JALCA)89(7)[Full Automatic 4000b/h 500ml Plastic Jar Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Made in China Lqa Strap Banding Tool]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cotton Yarn Computerized Lace Knitting Machine. Analytical Biochemistry212,436-445[Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application]

Dalev, P.G. and Simeonova, L.S.(1992),Plastic Caps Making Machine / Injection Molding Machine. Biotechnology letters 14,6, 531-534[China Modern Pharmacy Vacuum Filter Press]

Heidemann, E.(1997),Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate.Leder 48,(5),99-104[Binding Wire]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Folding Play Mat Crawl Play Mat]

Marine Mooring Hardware, Anchor Chain, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge.Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness. USP/SP[Dbry-320 Christmas Stickers Labels Printing Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Density Celuca Kitchen Cabinets PVC Foam Board]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fiber Optic Sc APC Fast Connector for FTTH Drop Cable]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[288w Curved Led Light Bar High Quality Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Self Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key]

Safest Baby Healthcare and Grooming Kit for Baby Gift, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Aluminum Foil PE Bottle Cap Induction Seal Liner]

Safest Baby Healthcare and Grooming Kit for Baby Gift,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Racing Preformance PTFE Teflon Alloy Fuel Adapter]

Safest Baby Healthcare and Grooming Kit for Baby Gift, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro, Part I.Micro DC 24V Worm Gear Brushless Motor for Medical Equipment. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length]

1.6 Ton Hand Lifting Tool Lever Chain Block, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CNC Machining Service High Precision CNC Mechanical Parts.Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks, 175-184[Top Valued Car Spray Paint Booth for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Robot of World Robotics Competition]

Whitaker,J.R.(1963),Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker. Analytical Chemistry35(12), 1950-1953[2mm Mirror Glass/Coated Glass for LED]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Food Machine Cube Ice Machine for Commercial UseAuthor for correspondence