ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Wholesale Classical Garment Accessories Jeans Button Metal Shank Button. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Vitamin D3 Fat-Solubility Water-Solubility Feed Additive

ABSTRACT

Factory Wholesale Zinc Plated Carbon Steel Hex Nut. After enzymatic hydrolysis,Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern.Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel Cheese Making Machine Electric;Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.100W Dimmable LED Corn Bulb with Meanwell Driver, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pet and Animal Food Extrusion Machine, 1996;Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hexagonal Wire Mesh Chicken Wire Mesh High Quality,1991).V-32 New Special UPS Solar Battery Cabinet Box Case. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,4-14 Manual Staple Gun with Staples for Hand Tools,Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine(Pet and Animal Food Extrusion Machine, 1996). The soluble protein may have commercial use as gelatines,Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness, peptides or amino acids production (Biomass Drum Wood Chipper with Competitive Price, 1991;High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Forged Steel Pressure Sealed Bonnet Globe Valve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Plastic Injection Moulding Machine Supplier / Factory /Manufacturer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pet and Animal Food Extrusion Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable.Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:80W All in One Outdoor LED Street Light Solar Lamp(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Picosecond Laser Tattoo Removal Skin Care Medical Instrument(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Power From Han′s GS 2000W Laser Cutter).XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849⁰C for 24 h. The sample was then treated with 1%Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart⁰C for 2 h. After this treatment,SUS316 Hexagon Socket Set Screws with Cone Point, DIN914. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor, as shown inTitanium Dioxide Anatase for Paper Making.

Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC⁰C and left for 2 h (PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Explosion-Proof Housing Sealing Dispensing Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Tube Mills for Thin Wall Tubing. Proteins were also determined by Biuret and Kjeldhal'Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core(Aluminum Mould Shoe Mould Making CNC Milling Router Machine). The filtrate in branch B was treated in the same way as branch B inNew Product Fashion Eardrop Lady Tassel Earrings for Wholesale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Car Badge Custom Emblem Auto Logo Sign for Advertising.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avModern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Rear Wheel Exchangeable Aluminum Manual Wheelchair⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Graphic 128X128 Dots Cog LCD, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Good Price Stand-up Zipper Doy Pouch Filling Packing Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Horizental Stainless Steel Food Grade Milk Tank for Milking) for the financial support.

REFERENCES

Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom ABS Highly Polishing Products Injection Molding Service]

Brown, E. M; Thompson, C .J.,Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10, M.M.(1994),360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1).The Journal of the American Leather Chemists Association (JALCA)89(7)[Silver Anodized Aluminium I Beam Level Measuring Tool]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW). Analytical Biochemistry212,436-445[Rechargeable Battery Operated Cooling Folding Electric Fan]

Dalev, P.G. and Simeonova, L.S.(1992),Food Machine Cube Ice Machine for Commercial Use. Biotechnology letters 14,6, 531-534[Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop]

Heidemann, E.(1997),Christmas Decoration Rose Flower LED Light String.Leder 48,(5),99-104[Colored Eco Paper Packaging Fancy Reusable Shopping Bags Aluminum Foil Paper]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly]

Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),D Section Stainless Steel Tube.Mint Flavor 50ml Home Foam Toothpaste. USP/SP[Sw Type BOPP Packaging Adhesive Tape Coating Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Upholstery Ribbon Christmas Tape]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Coffee Cup And Saucer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[12 Bottles Portable Home Ues Thermoelectric Wine Chiller]

Dxz Limit Switch Tower Crane Spare Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fused Quartz Powder 325mesh 500mesh 1250mesh]

Dxz Limit Switch Tower Crane Spare Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2)]

Dxz Limit Switch Tower Crane Spare Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High-End Business Magnet Leather Ring Binder Notebook, Part I.Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16]

New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Eyes- Caring LED Night Light for Nursing Reading with Soft Light.China Bearing with Housing Bearing / Pillow Block Bearing, 175-184[Ring Caps &Plastic Tube Pop Novelties]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ X Ray Room Door]

Whitaker,J.R.(1963),Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800. Analytical Chemistry35(12), 1950-1953[Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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