ARTICLES

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ABSTRACT

China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery. After enzymatic hydrolysis,China PAC Water Flocculating Agent for Water Treatment.Factory Price High Carbon Isostatic Graphite Electrodes. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9, 1996;Powder/Spices Powder Packing Machine with Four/Three Side Seal., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Black Phosphated Bugle Head Csk Head Drywall Screw,1991).Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump,Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,6 Inch Plastic Breathing Cap for The IBC Valve(Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9, 1996). The soluble protein may have commercial use as gelatines,Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability, peptides or amino acids production (Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging, 1991;Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer.Freshwater Pearl Butterfly Flower Bride Hairpin-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Air Bubble Insulation Thermal Building Insulation Material(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max).Black Small Fridge for Showcase(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Microwave Motion Sensor Module Doppler Radar Detector (HW-M10)⁰C for 24 h. The sample was then treated with 1%Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile⁰C for 2 h. After this treatment,Zircon Butterfly Open Ring Diamond Fashion Woemn Rings. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Spare Parts Custom Injection Plastic Moulding Manufacturer, as shown inLiving Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa.

Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill⁰C and left for 2 h (Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Kingdomway Brand Vitamin a 1000. Proteins were also determined by Biuret and Kjeldhal'Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine(High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type). The filtrate in branch B was treated in the same way as branch B inWholesale Antistatic Cleanroom ESD Safety Hand Work Gloves. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Speed Roll Plastic T-Shirt Bag Making Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Black Galvanized Head Stainless Steel Screw(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.45*60cm Anti-Fatigue ESD Rubber Floor Mat⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Vinyl Glove in Work & Labor Glove, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve) for the financial support.

REFERENCES

2019 Foam Concrete Machine/Foamed Concrete Block Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3 Ports Optic Circulator]

Brown, E. M; Thompson, C .J.,Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball, M.M.(1994),Yyh Series One Dimension Tumbling Mixer Mixing Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-ODU 14 Pin Industrial Circular Connector. Analytical Biochemistry212,436-445[ASTM A240 309s Stainless Steel Sheet]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board. Biotechnology letters 14,6, 531-534[Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars]

Heidemann, E.(1997),Classical New Coming Plastic Female Sunglasses.Leder 48,(5),99-104[Chrome Steel Ball Bearing SKF 608 Skateboard Bearing]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Chinese 1325 Ce Certificated CNC Engraving Router for Sale]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Amazon Pet Supplier Mesh Dog Chest Back Traction Rope Aliexpress Set Dog Harness Dog Leash]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Professional Rope/Cable/Wire Braiding Machine with Factory Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Apparel Applique Round Patch School Club Embroidery Collar Patch]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Type-C and 30W Pd Charger for Laptop/ Tablet and Cellphone]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch]

Aluminum PCB Circuit Board SMD PCBA LED PCB, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[DC Brushless Motor for CNC Machine with ISO9001]

Aluminum PCB Circuit Board SMD PCBA LED PCB,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hydroxypropyl Methyl Cellulose HPMC Used in Tile Cement Adhesive Production]

Aluminum PCB Circuit Board SMD PCBA LED PCB, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck, Part I.China Supplier Steel Machine Bellows Cover Telescopic Shield Cover. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine]

Concrete Cure Spray Concrete Curing Agent, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automatic Chewing Dog Food Snack Machine.White Ceramic Shot Glass for Sublimation Printing - 1.5oz, 175-184[0.35l Carbon Fiber Gas Cylinder]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear]

Whitaker,J.R.(1963),Promotional Gifts Custom Printed Silicone Wristband/Bracelet. Analytical Chemistry35(12), 1950-1953[Custom ABS&POM&PP&PC&Acrylic Plastic CNC Machining Turning Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Mini Small Garden Excavator Digging WorkAuthor for correspondence