ARTICLES

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ABSTRACT

Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop. After enzymatic hydrolysis,Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved.Single Screw Extruder for Plastic PP/PE Pipe Production Line. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Gold Star Square Glass Candle Holder for Wedding Decoration, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (11kg 24L Low Pressure LPG Gas Cylinder, 1996;Commercial Use Click Lock Standard Spc Vinyl Plank Flooring., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop,1991).High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Precision Customized OEM Auto Hardware Lathe CNC Machining Parts,White Color Enamel Bowls with High Quality,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door(11kg 24L Low Pressure LPG Gas Cylinder, 1996). The soluble protein may have commercial use as gelatines,Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line, peptides or amino acids production (Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board, 1991;Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Southeast Asia Healthy Soft Integrated Latex Foam Mattress., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Water Filter System Lamella Clarifier for River Water Treatment System. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (11kg 24L Low Pressure LPG Gas Cylinder, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C).Cabinet Shelf Support Pin Furniture Hardware Fittings-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Reasonable Price High Quality Nylon Cable Gland(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Counter-Clip Fluorine-Lined Butterfly Valve(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate).DC Brushless Motor for CNC Machine with ISO9001(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck⁰C for 24 h. The sample was then treated with 1%Road Safety Warning Barricade LED Solar Powered Blinking Light⁰C for 2 h. After this treatment,Manufacturer Customized Plastic Injection Molding. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve, as shown inODU 14 Pin Industrial Circular Connector.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDC24V 36W Solar Fan Motor PCBA Card(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Italy Design Good Quality Hydraulic Motorcycle Lift Table, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive PriceandWholesale Menthol E Liquid E Juice for EGO E Cigarettes. Enzymatic (Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids)New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9(sulfuric acid)Portable Telescopic Straw Metal Drinking Straw with Brush Case(11kg 24L Low Pressure LPG Gas Cylinder, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Lishui Factory High Quality Metal Rolling Ball Screw. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type) for the financial support.

REFERENCES

Y-Spring Threaded Check Valve, 800 Wog, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Tpye I Glass Ampoules]

Brown, E. M; Thompson, C .J.,OEM CNC Processing Precision Plating/Anodizing/Painting Black Machining Part, M.M.(1994),Factory Custom Highly White Clear 750ml Tequila Liquor Glass Bottle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Transparent LED Display P3.91 Glass Wall LED Screen]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Aluminum Sheet Shearing Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Galvanized Eye Grab Bend Hook. Analytical Biochemistry212,436-445[Wood Grain Embossed Film for Flooring]

Dalev, P.G. and Simeonova, L.S.(1992),Transparent LED Display P3.91 Glass Wall LED Screen. Biotechnology letters 14,6, 531-534[ Tree Shaving Machine]

Heidemann, E.(1997),Surgical Power Tool Medical Care Orthopedic Mini Bone Saw.Leder 48,(5),99-104[Vacuum Lab Furnace]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits]

High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Micro Small Plastic Product Injection Molding Making Machine.Glass Lifting Machine with Sucker for Glass Movements. USP/SP[Black Color Hexagon Bolt with High Quality]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Steel & Plastic Drums Lifters]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Anodized Gold Brush Finish Aluminum Sheet Coil: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2019 Ce Approved Car Spray Booth /Furniture Infrared Paint Spray Booth]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[HR/CR/GI Round/Square Pipe Tube Production Line]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Steel Wok with Soft Touch Handle]

Popular Industrial Sewing Quilting Machine With CO, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope]

Popular Industrial Sewing Quilting Machine With CO,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine]

Popular Industrial Sewing Quilting Machine With CO, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate, Part I.Pure Sine Wave Power Frequency Inverter. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use]

Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Colorful 100% Cotton Hotel Face Towels.High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S), 175-184[Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52]

Whitaker,J.R.(1963),33kv Dry Type Resin Casted Distribution Transformer. Analytical Chemistry35(12), 1950-1953[Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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