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ABSTRACT

3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board. After enzymatic hydrolysis,Low Pressure PU Filter Gasket Sandal Making Machine.Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ferrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh, 1996;Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Metal Material and Sports Theme Custom Metal Medal,1991).Portable Installation and Ce Certification 20g/Hr OEM Air Purifier. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3),Full Computer Plain Socks Knitting Machine with High Quality,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil(Ferrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh, 1996). The soluble protein may have commercial use as gelatines,New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z), peptides or amino acids production (One Step Myo/Ck-MB/Ctnl Combo Rapid Test Cassette, 1991;Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;OEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Natural Latex Pocket Coil Spring Mattress for 5 Star Hotel. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ferrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Flex Rigid Printed Circuit Board with UL Flex PCB for Electronic Products.Made in China Lqa Strap Banding Tool-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New R&D Wire Cable Feeding Winding Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Galvanized Roller Shutter Window Torsion Springs(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce).100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Carbon Graphite Crucible⁰C for 24 h. The sample was then treated with 1%Decorative Aluminum Solid Panel for Commercial Building⁰C for 2 h. After this treatment,Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Di DIN3352 Double Orifice Automatic Air Release Valve Dn65's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Magic Hair Twist   Curl Sponge Brush, as shown inManufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing.

Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75)⁰C and left for 2 h (Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Geyi Disposable Laparoscopic Visible Optical Trocar. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding. Proteins were also determined by Biuret and Kjeldhal'Bimetallic Thermometer Industrial Temperature Gauge(Southeast Asia Healthy Soft Integrated Latex Foam Mattress). The filtrate in branch B was treated in the same way as branch B inLow Cost Sawdust Rotary Carbonization Machine with Most Popular. The final soluble protein shown were used for peptides separations using gel permeation chromatography,19 Network Cabinet with Lockable Rear Door.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av175mm Diamond Cutting Blade for Granite&Tiles(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PU-Flex Duct Hose for Transporting Dust, Granules and Shavings⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Uprigth Touch-Screen Red Wine Chiller, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738), V_e, and K_avcalculated as discussed previously.Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen,High Tensile Strength Galvanized Steel Wire. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Milling Precision Stainless Steel CNC Machinery Parts) (Reis et al.,1989;One Step Myo/Ck-MB/Ctnl Combo Rapid Test Cassette.,1991;Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment.,1991).24 Volt Fire Alarm Strobe Light Horn Strobe Sounder(1992), were lower, except for cystine and tyrosine,from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder.

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CONCLUSIONS

Best Price PVC Coated Fiberglass Mesh Insect Window Screen. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Garden Decoration Marble Stone Granite Small Angel Sculpture Water Fountain) for the financial support.

REFERENCES

Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[1d Barcode Scanner Supplier & Factory]

Brown, E. M; Thompson, C .J.,Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor, M.M.(1994),Factory OEM CNC Machined Gear Motor Shaft.The Journal of the American Leather Chemists Association (JALCA)89(7)[Customized 8 Layers Round LED Panel Aluminum PCB for LED Light]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[33kv Dry Type Resin Casted Distribution Transformer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage. Analytical Biochemistry212,436-445[Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping]

Dalev, P.G. and Simeonova, L.S.(1992),Pink French Loop Terry Knit Fabric for Jacket. Biotechnology letters 14,6, 531-534[Festival Ceramic Mug]

Heidemann, E.(1997),Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb.Leder 48,(5),99-104[Polishing Best Quality Customized Steel Anodized CNC Turning Machining Auto Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mint Flavor 50ml Home Foam Toothpaste]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fly]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[machining-in-china]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cable Tray with ′t′ Slots]

Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Refrigeration Digital Temperature Controller Stc-100A]

Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss]

Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit, Part I.Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank]

Cute Pink Cashmere Soft Plush Lining Warm Bootie, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3kw 24V 310mm Vertical Agv Drive Wheel Assembly.Sofa Materials PU Leather with Flock Backing, 175-184[Flange Mounted High Torque Bonfiglioli Planetary Gearbox]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Good Prize Solar PV Mounting Panel System for Ground]

Whitaker,J.R.(1963),Three Phase 800mm Plate Fan. Analytical Chemistry35(12), 1950-1953[Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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