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ABSTRACT

Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks. After enzymatic hydrolysis,Counter-Clip Fluorine-Lined Butterfly Valve.DC Brushless Motor for CNC Machine with ISO9001. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck;Road Safety Warning Barricade LED Solar Powered Blinking Light; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Manufacturer Customized Plastic Injection Molding, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08), 1996;Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine,1991).60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Solar Energy Storad Power Generation System,110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Aluminum Cover Frame Half Aluminum Cover Profile(Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08), 1996). The soluble protein may have commercial use as gelatines,Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility, peptides or amino acids production (DC24V 36W Solar Fan Motor PCBA Card, 1991;New Monocular Hunting Wargame Mini Scope Digital Night Vision, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Italy Design Good Quality Hydraulic Motorcycle Lift Table. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Menthol E Liquid E Juice for EGO E Cigarettes.Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Lab Equipment Sr Paper Pulp Beating Freeness Test Machine, Parana State,New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126.CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the45*60cm Anti-Fatigue ESD Rubber Floor MatandColorful Pet Natural Tough TPR Rubber Toy Cat Dog Chew Toys.

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Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (15mm Best Price Commercial Grade Birch Plywood for Furniture).Popular Water Bike / Hydro Bike / Aqua Bike (SK-8001)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fertilizer Granulator Machine, Compound Fertilizer & Organic Fertilizer Granulator, Machine for Making⁰C for 24 h. The sample was then treated with 1%Double Roller Rotary Wood Peeling Machine with CE for Sale⁰C for 2 h. After this treatment,5in1 USB Type C Hub HDMI 4K USB C Hub to Ethernet RJ45 LAN Adapter. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sale Coral Fleece Hotel Bathrobe (DPFT8070)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone, as shown inGsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine.

Ladle Refining Melting Steel Furnace Casting Ladle⁰C and left for 2 h (Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Lishui Factory High Quality Metal Rolling Ball Screw. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Y-Spring Threaded Check Valve, 800 Wog. Proteins were also determined by Biuret and Kjeldhal'OEM CNC Processing Precision Plating/Anodizing/Painting Black Machining Part(201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet). The filtrate in branch B was treated in the same way as branch B inCheap Aluminum Truss Stage Light Frame. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Factory Custom Highly White Clear 750ml Tequila Liquor Glass Bottle.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Galvanized Eye Grab Bend Hook. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTransparent LED Display P3.91 Glass Wall LED Screen(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Micro Small Plastic Product Injection Molding Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Glass Lifting Machine with Sucker for Glass Movements, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Anodized Gold Brush Finish Aluminum Sheet Coil, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

ASTM A240 309s Stainless Steel SheetandPopular Industrial Sewing Quilting Machine With CO. Enzymatic (Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace)Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate(sulfuric acid)Pure Sine Wave Power Frequency Inverter(Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Colorful 100% Cotton Hotel Face Towels.Iridium Power Denso Spark Plug Bk6rei-11 for Honda Carsshows the peptides separations after enzymatic hydrolysis.High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChinese 1325 Ce Certificated CNC Engraving Router for Sale, but not shown in Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights,33kv Dry Type Resin Casted Distribution Transformer, and also free amino acids (Whitaker et al., 1963).Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08),1996).

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CONCLUSIONS

25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Etching Rear Trunk Sill Plates for Hyundai Tucson) for the financial support.

REFERENCES

Column Type Pave Rubber Tile Making Machine-Vulcanizing Press Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[API609 150lb/300lb Triple Eccentric Worm Gear Wafer Type Butterfly Valve]

Brown, E. M; Thompson, C .J.,Hot Sales in Amazon Store 18V Cordless Drill Vertical Battery Pack for Home Work DIY, M.M.(1994),3 12 Strand 3mm 4mm 5mm 10mm 18mm Braided Dan Line Plastic PP Packing Hollow Polypropylene Rope.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mini Small Garden Excavator Digging Work]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic. Analytical Biochemistry212,436-445[Disposable Anesthesia Breathing Circuit Tube]

Dalev, P.G. and Simeonova, L.S.(1992),Leisure Men′s Waterproof Strap Light Cross Body Anti-Theft Chest Bag. Biotechnology letters 14,6, 531-534[DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge]

Heidemann, E.(1997),Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels.Leder 48,(5),99-104[High Quality Food Processor Fruit Vegetable Swift Chopper]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[800W Electric Motor Price Lawn Mower Motor Concrete Mixer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hardware CNC Machinery Milling Part: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Compression Polyester Light Ankle Support Brace for Youth]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Investment Casting For Valves]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube]

Customized 8 Layers Round LED Panel Aluminum PCB for LED Light, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Road Construction Fabric Non-Woven Geotextile 200GSM]

Customized 8 Layers Round LED Panel Aluminum PCB for LED Light,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Geyi Disposable Laparoscopic Visible Optical Trocar]

Customized 8 Layers Round LED Panel Aluminum PCB for LED Light, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge, Part I.Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Advanced Unlocking Tool for Locksmith]

Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Linear Guideway for CNC Machine as Components.Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts, 175-184[Unisex Office Work Mans Winter Jackets]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China]

Whitaker,J.R.(1963),40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5). Analytical Chemistry35(12), 1950-1953[Top Great Wallet Genuine Leather Purse with Coin Pocket]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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