ARTICLES

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^IICNPq/RHAE/biotechnology-530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header

ABSTRACT

Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly. After enzymatic hydrolysis,Galvanized Roller Shutter Window Torsion Springs.100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Carbon Graphite Crucible;Decorative Aluminum Solid Panel for Commercial Building; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Di DIN3352 Double Orifice Automatic Air Release Valve Dn65, 1996;Magic Hair Twist   Curl Sponge Brush., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75),1991).Geyi Disposable Laparoscopic Visible Optical Trocar. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding,Bimetallic Thermometer Industrial Temperature Gauge,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,19 Network Cabinet with Lockable Rear Door(Di DIN3352 Double Orifice Automatic Air Release Valve Dn65, 1996). The soluble protein may have commercial use as gelatines,Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery, peptides or amino acids production (175mm Diamond Cutting Blade for Granite&Tiles, 1991;Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PU-Flex Duct Hose for Transporting Dust, Granules and Shavings., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Uprigth Touch-Screen Red Wine Chiller. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Di DIN3352 Double Orifice Automatic Air Release Valve Dn65, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator.Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Parcel Tape Adhesive OPP Packing Tape, Parana State,PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header.High Efficiency Non-Expensive Cracking Agent, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Di DIN3352 Double Orifice Automatic Air Release Valve Dn65, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHeat Resistant Pyrex (borosilicate) Glass Food Storage Containersand6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder.

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Fiber Optic Sc APC Fast Connector for FTTH Drop Cable(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Dn32 (11/4) BSPT Thread Air Actuated Piston Valves).Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Striped Compression Soccer Socks for Adults⁰C for 24 h. The sample was then treated with 1%Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738)⁰C for 2 h. After this treatment,Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Tensile Strength Galvanized Steel Wire's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)24 Volt Fire Alarm Strobe Light Horn Strobe Sounder, as shown in32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall.

from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder⁰C and left for 2 h (Chicken Cage Mesh Hexagonal Wire Mesh Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Best Price PVC Coated Fiberglass Mesh Insect Window Screen. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application. Proteins were also determined by Biuret and Kjeldhal'Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor(Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima). The filtrate in branch B was treated in the same way as branch B inPVDF Coated Aluminum Honeycomb Panel for Construction Materials. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Factory OEM CNC Machined Gear Motor Shaft.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPink French Loop Terry Knit Fabric for Jacket(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Roller Wedge Screen CylinderandElectric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600). Enzymatic (Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall)Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit(sulfuric acid)Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262)(Di DIN3352 Double Orifice Automatic Air Release Valve Dn65, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Cute Pink Cashmere Soft Plush Lining Warm Bootie, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Cute Pink Cashmere Soft Plush Lining Warm Bootie, flow rate of 0.1 mL/min., and l = 620 nm.

3kw 24V 310mm Vertical Agv Drive Wheel Assembly.Freshwater Pearl Butterfly Flower Bride Hairpinshows the peptides separations after enzymatic hydrolysis.Sofa Materials PU Leather with Flock Backing(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBoho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women, but not shown inElbow Forming Machine Gorelocker HVAC Duct Making Machine,Three Phase 800mm Plate Fan, and also free amino acids (Whitaker et al., 1963).ASTM Approved Popular Indoor Trampoline of Park. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Di DIN3352 Double Orifice Automatic Air Release Valve Dn65,1996).

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Customize Milky Extrusion PC CoverandAnti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chairshow the gel chromatographic separation of soluble protein with Sephadex G-Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building.Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light, and another peak at 45 mL not shown inVertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine, for peptides lower than 3000 Da.Wholesale Genuine Leather Credit Card Mens Wallet(Condell et al.,1993) .

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Yitai Die Making 13mm Beech Rotary Plywood, V_e, and K_avcalculated as discussed previously.Gear Rolling Machine Zh28/25 Spline Hobbing Machine,OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321)) (Reis et al.,1989;175mm Diamond Cutting Blade for Granite&Tiles.,1991;Magic Hair Twist   Curl Sponge Brush.,1991).Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale(1992), were lower, except for cystine and tyrosine,Partial Thread Steel/Iron Hex Head Self Tapping Screw.

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CONCLUSIONS

Flat Car Sun Roof Tent Awning. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality CE Digital Display Adjustable Power Supply) for the financial support.

REFERENCES

Classic Black Color Commercial Glass Door Multi Deck Refrigerator, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[40tons High Speed EVA Die Cutting Machine (HG-A40T)]

Brown, E. M; Thompson, C .J.,Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits, M.M.(1994),12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026).The Journal of the American Leather Chemists Association (JALCA)89(7)[Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Designed Steel Frame Factory Building]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pneumatic Control Shuttle Valve NPT Thread. Analytical Biochemistry212,436-445[China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW]

Dalev, P.G. and Simeonova, L.S.(1992),Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing. Biotechnology letters 14,6, 531-534[By6-8-200-L Automatic Doypack Premade Pouch Packing Machine]

Heidemann, E.(1997),OEM 15PCS Electric Face Makeup Brush with Ce Certificate.Leder 48,(5),99-104[Cable / Adapter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[6 Inch Plastic Breathing Cap for The IBC Valve]

Garden Decoration Marble Stone Granite Small Angel Sculpture Water Fountain, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),New Styles Fashion Erotic Women Underwear Sexy Lingerie.on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale. USP/SP[Black Tko Wholesale Vape Carts 0.8ml Empty Tank Vape Cartridges Atomizer 510 Thread Sauce Carts]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Customized Chilled Cast Iron Roll]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stand Hot and Cold Normal Water Dispenser with Refrigerator: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Water Slide for Indoor Play Structure]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2/18 Inch Spiral Welded Stainless Steel Pipe Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PTZ Camera Two-Way  Audio  Baby Monitor Simple Easy to Operate]

High Quality Construction Welding Rods Electrode E6013 E7018 E7016, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Tct Circular Saw Blade for Grass Cutting]

High Quality Construction Welding Rods Electrode E6013 E7018 E7016,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard]

High Quality Construction Welding Rods Electrode E6013 E7018 E7016, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular, Part I.Lydite Fence Reel Reel with Extension Arms for Farm Fencing. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization)]

1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Customized 3D Medal for Religious Medal Gift.Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules, 175-184[304 316 Seamless Polished Stainless Steel Bright Annealed Tube]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe]

Whitaker,J.R.(1963),Dbry-320 Christmas Stickers Labels Printing Machine. Analytical Chemistry35(12), 1950-1953[Metal Gold Marathon Running Sport Medal for Promotion Gift]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

FUJI Home Monarch Hotel Passenger Elevator Residential Building ElevatorAuthor for correspondence