ARTICLES

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^IICNPq/RHAE/biotechnology-Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer

ABSTRACT

Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail. After enzymatic hydrolysis,High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine.Stainless Steel Clarifier Tank for Water Treatment. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Carrier Roller for Sany Excavator;LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts, 1996;Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002),1991).2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain,Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Dry Air Generator GF Series(Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts, 1996). The soluble protein may have commercial use as gelatines,Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture, peptides or amino acids production (Advanced Unlocking Tool for Locksmith, 1991;Screen Printing Materials and Equipments Screen Printing Frames, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Supplier Hard Ware Fittings Gas Spring for Tool Box., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet.Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss, Parana State,Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer.Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theM4 Pneumatic Air Driven Liquid Booster Pump Various Good QualityandFashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security.

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PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Best Selling Quality Aluminum Acetylene Gas Cylinder).Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Speed Copper Wire Braiding Making Machine⁰C for 24 h. The sample was then treated with 1%Safe Durable Construction Scaffolding Plank for Building⁰C for 2 h. After this treatment,Different Size of Ring NdFeB Magnet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Welding Structure, as shown in Anchor Chain.

Carboxymethyl Cellulose CAS No. 9004-32-4⁰C and left for 2 h (Hotel SPA Swimming Pool). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip. Proteins were also determined by Biuret and Kjeldhal'150kg Per Hour Frozen French Fries Production Line(High Polymer Modified Cement Based Elastic Waterproof Coating). The filtrate in branch B was treated in the same way as branch B inOutdoor Fiber Optic Drop Cable Factory & Manufacturer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Automatic Professional LED Display Ceramic Hair Curling Iron.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Omni Newest Manager Desk Office Smart Office Table Office Staff Table, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filterand6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap. Enzymatic (30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps)Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage(sulfuric acid)Full-Auto CNC Cutting Machine for Architecture Glass Sheet(Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product, flow rate of 0.1 mL/min., and l = 620 nm.

Hot Forming Pushing Elbow Making Machine.3D Laser Inside Engraving Machinery Use Germany Technology with High Speedshows the peptides separations after enzymatic hydrolysis.13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inElectric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X), but not shown inPicc Puncture Care Kit,Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner, and also free amino acids (Whitaker et al., 1963).As568 Standard Brown FKM Rubber O Ring for Sealing. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts,1996).

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CONCLUSIONS

Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (AISI 201/202/301/304 Stainless Steel Welded Square Pipe) for the financial support.

REFERENCES

High Borosilicate Glass Baking Dish Set Clear, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Thermal Rockwool Sandwich Board for Commercial Building]

Brown, E. M; Thompson, C .J.,G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen, M.M.(1994),Embossing Roller for Tissue Paper.The Journal of the American Leather Chemists Association (JALCA)89(7)[ANSI C29 25kv 1pin Hole F Neck Modified Polyethylene HDPE Insulator]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Parcel Tape Adhesive OPP Packing Tape]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel. Analytical Biochemistry212,436-445[Single Screw Extruder for Plastic PP/PE Pipe Production Line]

Dalev, P.G. and Simeonova, L.S.(1992),Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates. Biotechnology letters 14,6, 531-534[Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief]

Heidemann, E.(1997),Colorful  Reflective Sticker Reflective Tape.Leder 48,(5),99-104[Garbage Bag On Roll]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products]

Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Concrete Spare Part Electrical System Central Distribution Box.Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress. USP/SP[China Supplier Hard Ware Fittings Gas Spring for Tool Box]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Three Phase 800mm Plate Fan]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-50Hz to 60Hz Frequency Inverter for Water Pump: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hard Roller Shutter with Good Thickness and Hardness (HF-J17)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Brushed Anodizing Aluminum Extrusion Profile for LED]

Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM]

Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Customized Precision Hydraulic Cylinder Metal Machinery Parts for Forklift]

Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels, Part I.Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror]

PVC Cabinet Construction Board, White 18mm PVC Foam Board, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2019 New Portable Mini Dental X-ray Machine.Ss Reduced Port 2000wog 1PC Screwed Ball Valve, 175-184[Android Bluetooth Beacon High Quality Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Spare Parts for Caterpillar Construction Machinery]

Whitaker,J.R.(1963),China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous. Analytical Chemistry35(12), 1950-1953[New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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