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ABSTRACT

China Prefab Steel Building for Garment Factory. After enzymatic hydrolysis,Dark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile.Cable Tray with ′t′ Slots. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet, 1996;Disposable PS Foam Fast Food Box Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A),1991).Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Foska Good Quality Plastic Stapler Set with Staples,Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hotel SPA Swimming Pool, Sport Room, Bathroom Towel(201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet, 1996). The soluble protein may have commercial use as gelatines,Powerful Cleaning Colorful Scouring Pad for Kitchen Use, peptides or amino acids production (500t Plywood Hot Press Woodworking Machine (12-25 layers), 1991;10ml Round Clear Nail Polish Bottle with Brush Cap, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Full Automatic 4000b/h 500ml Plastic Jar Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-5L, 8L, 10L, 20L, 50L, 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector.Hermetic Sliding Lead Door, X Ray Room Door-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Laundry Used Gas Water Heater with CE/Asme Certificate(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh, Parana State,China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous.24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8)andLow Smoke and Halogen Free XLPE Wire UL3266 32-10AWG.

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Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Spare Parts Custom Injection Plastic Moulding Manufacturer).What′s Kind of Machine for Making Organic Fertilizer Granules(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine⁰C for 24 h. The sample was then treated with 1%Home and Hospital Electric Patient Lift⁰C for 2 h. After this treatment,Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts, as shown inSheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality.

DC 12V Power Relay for Smart House⁰C and left for 2 h (Food Grade PTFE Coated Fiberglass Mesh Baking Sheet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Medical Human Body Thermometer. Proteins were also determined by Biuret and Kjeldhal'Patent Product Solar Insect Killer Lamp, Made in Goldsun, China.(Road Safety Warning Barricade LED Solar Powered Blinking Light). The filtrate in branch B was treated in the same way as branch B inSpin Typical Semi-Automatic Car Wash Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Greenhouse Fixing Film Profile Lock Channel and Spring Wire.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avExport Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Prefab Expandable Luxury Living Container House Prefabricated HouseandChina Supplier Granular Coal Based Activated Carbon Filter Meida. Enzymatic (Glass Cutting Table/CNC Glass Cutting Machine)12 Bottles Portable Home Ues Thermoelectric Wine Chiller(sulfuric acid)Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter(201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321), flow rate of 0.1 mL/min., and l = 620 nm.

Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap.Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truckshows the peptides separations after enzymatic hydrolysis.Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDeep Hole Cylinder Boring Machine, but not shown inDog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage,Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle, and also free amino acids (Whitaker et al., 1963).Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet,1996).

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Stainless Steel Barbecue Net BBQ Grill Grates Wire MeshandHigh Quality Cotton Cover with Polyester Microfiber Filling Comforter Duvetshow the gel chromatographic separation of soluble protein with Sephadex G-Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger.Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve, and another peak at 45 mL not shown in3kw 24V 310mm Vertical Agv Drive Wheel Assembly, for peptides lower than 3000 Da.Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron(Condell et al.,1993) .

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Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch, V_e, and K_avcalculated as discussed previously.High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt,Hanwha SMT Chip Shooter LED Pick and Place Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Big Chocolate Storage Holding Tank) (Reis et al.,1989;500t Plywood Hot Press Woodworking Machine (12-25 layers).,1991;Disposable PS Foam Fast Food Box Making Machine.,1991).ACP Aluminium Cmposite Panel Cladding Extrusions(1992), were lower, except for cystine and tyrosine,New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine.

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CONCLUSIONS

Wholesale Custom Mens Heated Down Jacket. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Accept OEM New Products Team Various Colors Gold Ribbon) for the financial support.

REFERENCES

Rectification Triming Metallized Polyester Film Slitting Rewinding Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Asa-100 High Pressure Breathing Air Compressor Supplier & Factory]

Brown, E. M; Thompson, C .J.,Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker, M.M.(1994),New Swim Shorts Grey Mens Board Shorts with Mesh Liner.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Low Price Top Selling Fashion Summer Boy Sandals]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Styles Fashion Erotic Women Underwear Sexy Lingerie]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control. Analytical Biochemistry212,436-445[Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans]

Dalev, P.G. and Simeonova, L.S.(1992),Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization). Biotechnology letters 14,6, 531-534[Light Weight Polyester Eye Mask for Travel Airplane Hotel]

Heidemann, E.(1997),Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil.Leder 48,(5),99-104[2-Tier Metal Spice Rack Wall Mount Jars Bottle Storage Shelf Holder Seasoning Organizer for Kitchen]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Fashion Cup Mat Silicone Rubber Coaster for Tea- Best Housewarming Beverage]

2019 New Portable Mini Dental X-ray Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Marine Mooring Hardware, Anchor Chain.Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls. USP/SP[Cute Pink Cashmere Soft Plush Lining Warm Bootie]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fully Automatic Dust Face Mask Making Machine with Ultrasonic]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Locking Hinge Pin]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[3.0mm Dust Mask Earloop]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Customized Marathon Medal for Marathon Medallion]

QJ-M Moving small manual brick making machine with low cost, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting]

QJ-M Moving small manual brick making machine with low cost,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Permanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets]

QJ-M Moving small manual brick making machine with low cost, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set, Part I.Braiding Machine for Weaving Flexible Metal Hose. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Kingdomway Brand Vitamin a 1000]

Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fashion Metal Button Crystal Shank Button for Garment.Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616), 175-184[Professional Factory High Accuracy Personal Anti-Theft Alarm Kl-Xb01]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine]

Whitaker,J.R.(1963),10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light. Analytical Chemistry35(12), 1950-1953[Adhesive Powder for Skim Coat/Wall Putty HPMC]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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