ARTICLES

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ABSTRACT

OEM Refrigeration Digital Thermometer. After enzymatic hydrolysis,BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L).Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sale 25kw Grid Tied Home Solar Panel System Kit, 1996;Mini Car Portable Fridge 10L Mini Fridge., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke,1991).Mini Small Garden Excavator Digging Work. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Professional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver,Offshore Drilling Platform Air Winch for Marine Oilfields,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Mass Stock Yl10.2 Carbide Rods for Milling 45HRC(Hot Sale 25kw Grid Tied Home Solar Panel System Kit, 1996). The soluble protein may have commercial use as gelatines,Heavy Industry Electric Transport Vehicle on Rails, peptides or amino acids production (Manual Interlocking Brick Making Machine, 1991;A100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Direct Factory Suppy Electric Razor Barbed Wire Low Price for Fencing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Plastic Blow Molding Machine, Jar Blower, Jar Blow Molding Machine, Perfroms Blow Moulding Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sale 25kw Grid Tied Home Solar Panel System Kit, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Metal Gold Marathon Running Sport Medal for Promotion Gift.12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Security Shopping Trolley Baby Seat for Supermarket Cart(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Original Testo 174t Mini Temperature Data Logger No. 0572 1560).Hot Sale High Quality Rimless New Italy Design Titanium Eyeglasses for Business Men and Women(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%CH Series Pid Intelligent Temperature Controller CH402⁰C for 24 h. The sample was then treated with 1%China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory⁰C for 2 h. After this treatment,Disposable Precision Infusion Set with Filter and Needle for Single Use. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Low Price Sweep Frequency Response Analyzer for Power Transformer Test's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500, as shown inVitamin D3 Fat-Solubility Water-Solubility Feed Additive.

Stainless Steel Mechanic Parts for Lock Accessories with MIM Process⁰C and left for 2 h (Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Mining Machinery Sand Vibrating Sieve for River Sand. Proteins were also determined by Biuret and Kjeldhal'Straight-Through Medium Flange Hard-Sealed Z41h Gate Valve(460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric). The filtrate in branch B was treated in the same way as branch B inSanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,PRO Audio Loudspeaker PA Speaker System Line Array Speaker.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Palet Coil Nail Welding Machine/Nail Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCopy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hotel Bedding⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Supplier V Wedge Wire Johnson Water Well Screen, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Clear Black Empty 15ml 30ml 50ml 100ml Frosted White Square Glass Perfume Bottles, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Dz/Bt-80h Multifunctional Full Automatic Cartoning Machineand10A MPPT Solar Controllers PV 60V for off-Grid. Enzymatic (100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears)Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole(sulfuric acid)60ml 100ml Customized Glass Jar for Spice, Candle, Candy(Hot Sale 25kw Grid Tied Home Solar Panel System Kit, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price, flow rate of 0.1 mL/min., and l = 620 nm.

Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line.Hot Sale Factory stainless Steel Dining Chair OEM Dining Furnitureshows the peptides separations after enzymatic hydrolysis.Factory Outlet Automatic Grain Tower Dryer Drying Equipment(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inIgnition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849, but not shown in100W 120W 150W Outdoor Solar LED Street Lighting,Mesh Bag Packing 20kg Pure White Garlic, and also free amino acids (Whitaker et al., 1963).Supreme 524 Mv Sea Kayak. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Sale 25kw Grid Tied Home Solar Panel System Kit,1996).

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CONCLUSIONS

Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel) for the financial support.

REFERENCES

Laptop Bag College Men Backpack Leather Back Pack Bags, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[304/304L Stainless Steel Seamless Tubing/Metal Pipe Manufacturer]

Brown, E. M; Thompson, C .J.,Pet Dog Grooming Rake Dematting Tool Shedding Comb, M.M.(1994),Custom Apparel Applique Round Patch School Club Embroidery Collar Patch.The Journal of the American Leather Chemists Association (JALCA)89(7)[CNC System Air Duct Line5 Make Machine for HVAC]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Famous Brand Luxury Packaging Box Gift Box]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery. Analytical Biochemistry212,436-445[Full-Auto CNC Cutting Machine for Architecture Glass Sheet]

Dalev, P.G. and Simeonova, L.S.(1992),CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058. Biotechnology letters 14,6, 531-534[Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall]

Heidemann, E.(1997),Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror.Leder 48,(5),99-104[Medical Face Mask]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.xcfrfpc.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Customized Souvenirs Tin Metal Number & Name Plate for Car]

Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[XPS Extruded Polystyrene Foam Board]

Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom Rubber Product Seal O-Ring /NBR70 O Ring]

Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:White Cotton Satin Hotel Duvet Cover (DPFB8055), Part I.Small Cleaning Brush ESD Anti Static Brush ESD Brush Black. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Modern 4 Person Workstation with Computer Desk Modular Office Furniture]

PAC /Poly Aluminium Chloride for Water Treatment, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Aluminum Sheet Shearing Machine.Electrical PV DC Solar Cable, 175-184[Commercial 15W 20W 30W COB LED Track Light for Shop Supermarket Gallary Museum]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board]

Whitaker,J.R.(1963),Tomato Bubble and High Pressure Washing Machine. Analytical Chemistry35(12), 1950-1953[Locksmith Tool Car Lock Transpopnder Chip Key Blank Cover]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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