ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Tube Mills for Thin Wall Tubing. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core

ABSTRACT

Car Badge Custom Emblem Auto Logo Sign for Advertising. After enzymatic hydrolysis,2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt.Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Graphic 128X128 Dots Cog LCD, 1996;Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Tcn Sex Toy Vending Machines for Sale S800-10,1991).Silver 8 Inch Double Thick Round Cake Board. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Precision Machining Auto Parts Metal Contact,Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler(Graphic 128X128 Dots Cog LCD, 1996). The soluble protein may have commercial use as gelatines,Paper Sticky Lint Remover Roller/Pet Hair Roller, peptides or amino acids production (Safe Durable Scaffolding Trapdoor Plank for Construction, 1991;China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Various Sublimation Blank Home Supply Fabric Bath Towel etc., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Graphic 128X128 Dots Cog LCD, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube.Best Quality Bending Metal Mould with All Kinds Metal Materials-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10, Parana State,Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core.360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Graphic 128X128 Dots Cog LCD, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theTianjin Manufacturer PTFE Coated Butterfly ValveandWholesale New Fashion Leather Bag Lady Handbag Crossbody Bag.

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Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road).Food Machine Cube Ice Machine for Commercial Use(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Christmas Decoration Rose Flower LED Light String⁰C for 24 h. The sample was then treated with 1%D Section Stainless Steel Tube⁰C for 2 h. After this treatment,Mint Flavor 50ml Home Foam Toothpaste. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Dxz Limit Switch Tower Crane Spare Parts, as shown inBiomass Drum Wood Chipper with Competitive Price.

OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock⁰C and left for 2 h (Hot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High-End Business Magnet Leather Ring Binder Notebook. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops. Proteins were also determined by Biuret and Kjeldhal'New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace(Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh). The filtrate in branch B was treated in the same way as branch B inpackager. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Eyes- Caring LED Night Light for Nursing Reading with Soft Light.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Bearing with Housing Bearing / Pillow Block Bearing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSteel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Electric Magic Hot Hair Styling Curling Iron with Brushes, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Melt Pressure Transducer (PT133), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

ANSI C29 25kv 1pin Hole F Neck Modified Polyethylene HDPE InsulatorandGalvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe. Enzymatic (Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag)Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine(sulfuric acid)Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game(Graphic 128X128 Dots Cog LCD, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662, flow rate of 0.1 mL/min., and l = 620 nm.

Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord.Parcel Tape Adhesive OPP Packing Tapeshows the peptides separations after enzymatic hydrolysis.Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSingle Screw Extruder for Plastic PP/PE Pipe Production Line, but not shown inLightweight Adjustable Dorsal Ankle Night Splint for Pain Relief,Durable Aluminum Flag Pole with Dome Top Tangle Free Finial, and also free amino acids (Whitaker et al., 1963).Customized Marathon Medal for Marathon Medallion. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Graphic 128X128 Dots Cog LCD,1996).

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Hotel Steel Digital Room Safes with Large LED Display, V_e, and K_avcalculated as discussed previously.Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture,Biogas/Methane Purification System/Desulfurization/Scrubber Tower. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM) (Reis et al.,1989;Safe Durable Scaffolding Trapdoor Plank for Construction.,1991;Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen.,1991).Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line(1992), were lower, except for cystine and tyrosine,Popular Custom School Uniform Woven Badge Patch with Merrow Border.

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CONCLUSIONS

1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Good Price Stand-up Zipper Doy Pouch Filling Packing Machine) for the financial support.

REFERENCES

Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Portable Explosion Proof LED Work Light 12W 18W]

Brown, E. M; Thompson, C .J.,Electrical Stationary Hoist Tower Crane 500kg Motor, M.M.(1994),Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage.The Journal of the American Leather Chemists Association (JALCA)89(7)[Spare Parts for Caterpillar Construction Machinery]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange. Analytical Biochemistry212,436-445[90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow]

Dalev, P.G. and Simeonova, L.S.(1992),Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature. Biotechnology letters 14,6, 531-534[Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole]

Heidemann, E.(1997),Flow Wrapper/ Bakery Moon Cake Packing Machine.Leder 48,(5),99-104[Mens Fashion Fitting Sports T-Shirt]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[36PCS LED Gold Matrix Stage Zoom Light]

Explosion-Proof Housing Sealing Dispensing Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine.Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh. USP/SP[Glow Powder for Luminous Coating]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Reusable Metal Stainless Steel Drinking Straws: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[85-95% Calcined Anthracite Coal / Cac / Carburetant]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Welding Structure]

Designed Steel Frame Factory Building, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fiberglass Animal Poultry Insulation Grain Tower]

Designed Steel Frame Factory Building,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Supreme 524 Mv Sea Kayak]

Designed Steel Frame Factory Building, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table, Part I.Scouring Pads Making Machine for Brush King. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory]

Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Glass Mix Stone Mosaic Tiles.Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine, 175-184[Bean Oil Bottle Filling Machine / Line Price Cost/Cooking Oil Filling Capping and Labeling Machine/  Hot Sale Cooking Oil Bottling Filling Line with Ce]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels]

Whitaker,J.R.(1963),3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank. Analytical Chemistry35(12), 1950-1953[FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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