ARTICLES

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ABSTRACT

China Bearing with Housing Bearing / Pillow Block Bearing. After enzymatic hydrolysis,Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800.The Most Competitive Sodium Gluconate for Water Reducer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles;Electric Magic Hot Hair Styling Curling Iron with Brushes; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Melt Pressure Transducer (PT133), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe, 1996;Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine,1991).Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662,Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck(Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe, 1996). The soluble protein may have commercial use as gelatines,Durable Aluminum Flag Pole with Dome Top Tangle Free Finial, peptides or amino acids production (Customized Marathon Medal for Marathon Medallion, 1991;Price of a Retail Supermarket Push Cart for Sale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Lady Kids Child Yoga Clothes Gym Fitness Sports Wear.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hotel Steel Digital Room Safes with Large LED Display. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture.Biogas/Methane Purification System/Desulfurization/Scrubber Tower-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage, Parana State,Eyes- Caring LED Night Light for Nursing Reading with Soft Light.Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theACP Aluminium Cmposite Panel Cladding ExtrusionsandHotel Bedding.

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Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Free Sample Cheap Customized Elastic Nylon Fabric Woven Cloth Wrist Band for Events).Flow Wrapper/ Bakery Moon Cake Packing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine⁰C for 24 h. The sample was then treated with 1%Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh⁰C for 2 h. After this treatment,Reusable Metal Stainless Steel Drinking Straws. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Designed Steel Frame Factory Building's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate, as shown inGlass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine.

0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table⁰C and left for 2 h (China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Scouring Pads Making Machine for Brush King. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor. Proteins were also determined by Biuret and Kjeldhal'Glass Mix Stone Mosaic Tiles(Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8)). The filtrate in branch B was treated in the same way as branch B inTI. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChampagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Racing Preformance PTFE Teflon Alloy Fuel Adapter, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Leak ProofandHigh Quality Stainless Steel Expanded Metal Mesh. Enzymatic (Popular Lovely Animal Design Inflatable Slide for Kids)Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware(sulfuric acid)GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable(Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Hairs Comb for Pet Dog Cats, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Hairs Comb for Pet Dog Cats, flow rate of 0.1 mL/min., and l = 620 nm.

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Roller Wedge Screen Cylinder, V_e, and K_avcalculated as discussed previously.Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup,Push Pull Small Cable to Cable Connector. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts) (Reis et al.,1989;Customized Marathon Medal for Marathon Medallion.,1991;Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag.,1991).Stainless Steel Seamless Pipe ASTM A312 TP304 316(1992), were lower, except for cystine and tyrosine,Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco.

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CONCLUSIONS

Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter) for the financial support.

REFERENCES

Big Chocolate Storage Holding Tank, Chocolate Buffer Tank, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Perfum Bottle Luxury Glass]

Brown, E. M; Thompson, C .J.,Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce, M.M.(1994),Asco 353 Series 3/4 Pulse Valve Solenoid Valve.The Journal of the American Leather Chemists Association (JALCA)89(7)[Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material. Analytical Biochemistry212,436-445[Mass Stock Yl10.2 Carbide Rods for Milling 45HRC]

Dalev, P.G. and Simeonova, L.S.(1992),Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle. Biotechnology letters 14,6, 531-534[Size 000 Clear Pullulan Vegetarian Capsules]

Heidemann, E.(1997),Purple UL 1095 16AWG Electronic Lead Wire.Leder 48,(5),99-104[Solar Pump System]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments]

Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Brushed Anodizing Aluminum Extrusion Profile for LED.PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps. USP/SP[Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-S235jo S235jr Steel Pipe Tube Shs Rhs: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ferrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Embroidered Patches For Decoration]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Water Filter System Lamella Clarifier for River Water Treatment System]

Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pre-Seasoned Cast Iron Mini Oval Casserole Pot-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Durable Aluminum Flag Pole with Dome Top Tangle Free Finial]

Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Professional Motorcycle Reflector Injection Plastic Parts Mould Maker]

Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Square Wire Mesh Netting with SGS, Part I.Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Full Computer Plain Socks Knitting Machine with High Quality]

Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Outdoor Garden Save Energy with Solar Flame Wall Light.CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm), 175-184[Metal Casting Sports Medals Factory OEM/ODM Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket]

Whitaker,J.R.(1963),Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag. Analytical Chemistry35(12), 1950-1953[Professional Factory Supply Design Cat Tree Pet Product]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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