ARTICLES

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ABSTRACT

4 Axis CNC Woodworking Machinery Engraver Router. After enzymatic hydrolysis,Sports Mini Earbuds Tws Wireless Headphones in-Ear Bluetooth Headset with Charging Case V5.0.Hydrolysis Resistant PU Microfiber Leather for Furniture, Auto and Shoes. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Corrosion Resistant Waterborne Epoxy Resin on Metal Surface with We-8216;Pre-Seasoned Cast Iron Baking Stone Cookware; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Compression Polyester Light Ankle Support Brace for Youth, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Good Quality White PVC Rigid Foam Board for Iran Market, 1996;Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Carbon Raiser of Graphitized Petroleum Coke/GPC for Iron Casting,1991).High Precision Piston Ring Good Shape Ability High Quality Surface. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002,Farm Relief Plastic Automatic Air Release Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price(Good Quality White PVC Rigid Foam Board for Iran Market, 1996). The soluble protein may have commercial use as gelatines,Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry, peptides or amino acids production (Square Decorative Stainless Steel Crimped Wire Mesh, 1991;Mold Injection Molding Plastic Products White PC Housing, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sw Type BOPP Packaging Adhesive Tape Coating Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Metric Female Flat Seat Hose Fittings.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Good Quality White PVC Rigid Foam Board for Iran Market, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Locksmith Tool Car Lock Transpopnder Chip Key Blank Cover.Multi Color Spade Made of Steel and Panting-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Must 4kw 2HP Pump Power Inverter Ags Bts Available(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Dipped Galvanized Oval Wire Brazil).K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hydraulic Hose Flange Adapter Carbon Steel Forging Flange Coupling⁰C for 24 h. The sample was then treated with 1%Hot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts⁰C for 2 h. After this treatment,100W 120W 150W Outdoor Solar LED Street Lighting. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody, as shown inHans Factory Direct Sale Colorful Wholesale Beaded Lace Fabric.

Economic Full Automated SMT Production Line PCB Production Machinery⁰C and left for 2 h (Clear Black Empty 15ml 30ml 50ml 100ml Frosted White Square Glass Perfume Bottles). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket. Proteins were also determined by Biuret and Kjeldhal'Bank Safe Lock, Gun Cabinet Lock, Safe Lock (AL-206)(Yc Pure White Nylon Powder for 3D Printing). The filtrate in branch B was treated in the same way as branch B inHospital Five Differential 5 Part Hematology Analyzer (HA-7000). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%HDPE Geomembrane Lining Price for Pond Liner Film. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2.0mm Yellow Markers for Fiber Optic Multi Patch Cord⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Chinese Galvanized Rectangular Tube for Buildings Materials, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Customized Chilled Cast Iron Roll, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting.CNC Turning Centershows the peptides separations after enzymatic hydrolysis.Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPopular Industrial Sewing Quilting Machine With CO, but not shown inStainless Steel Seamless Pipe ASTM A312 TP304 316,Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#, and also free amino acids (Whitaker et al., 1963).Animal Agricultural Damping Horse Stall Rubber Cow Mat. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Good Quality White PVC Rigid Foam Board for Iran Market,1996).

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CONCLUSIONS

100% Cotton Luxury Printed Bedding Comforter Set. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stainless Steel Hinge Pin, Locking Hinge Pin) for the financial support.

REFERENCES

Mouse Pad with Gel Wrist Rest, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[New Intelligent Integrated Lockstitch Sewing Machine Direct Drive Only]

Brown, E. M; Thompson, C .J.,Custom Size Christmas Gift Personalized Watch Boxes for Men, M.M.(1994),Best Selling Quality Aluminum Acetylene Gas Cylinder.The Journal of the American Leather Chemists Association (JALCA)89(7)[Galvanized Pressure Locked Grating]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Coal Mine Mini Electric Excavator with Quick Coupling. Analytical Biochemistry212,436-445[Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck]

Dalev, P.G. and Simeonova, L.S.(1992),2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank. Biotechnology letters 14,6, 531-534[Various Types of Plastic Hooks Mould From Manufacturers]

Heidemann, E.(1997),Food Grade Transparent Cookie Airtight Plastic Container Jars.Leder 48,(5),99-104[Fire Fighting Rubber Boots]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie]

CNC 3 Axis Milling Machining Center, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory.Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women. USP/SP[Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Good 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated Glass]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Direct Sale Single Cloth Hanger Hook: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Home Machine 2019 New Commercial Treadmill Gym Equipment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[100% Acrylic Knitted Jacquard Fans Soccer Football Scarf]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Portable Mini Fridge with Light Box]

2 Round Sash Pulley for Chain - Brass (PVD), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Anodized Aluminum Expanded Metal Mesh Competitive Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern]

2 Round Sash Pulley for Chain - Brass (PVD),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Pressure Braided Rubber Oil Hose]

2 Round Sash Pulley for Chain - Brass (PVD), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot DIP Galvanized G2150 D Ring Shackle, Part I.Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe]

Round Shape Aluminum PCB and PCBA, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),36PCS LED Gold Matrix Stage Zoom Light.Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China, 175-184[China Acupressure Mat With Magnets Pyramids]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl]

Whitaker,J.R.(1963),Chicken Cage Mesh Hexagonal Wire Mesh Machine. Analytical Chemistry35(12), 1950-1953[10 Gauge Galvanized Welded Wire Mesh for Construction Using]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Tensile Strength Galvanized Steel WireAuthor for correspondence