ARTICLES

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ABSTRACT

Rectification Triming Metallized Polyester Film Slitting Rewinding Machine. After enzymatic hydrolysis,Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker.New Swim Shorts Grey Mens Board Shorts with Mesh Liner. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Marine Mooring Hardware, Anchor Chain, 1996;Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick,1991).QJ-M Moving small manual brick making machine with low cost. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device,Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Braiding Machine for Weaving Flexible Metal Hose(Marine Mooring Hardware, Anchor Chain, 1996). The soluble protein may have commercial use as gelatines,Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking, peptides or amino acids production (Fashion Metal Button Crystal Shank Button for Garment, 1991;Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-ABC-508 Brake System Cleaning and Fluid Exchanging Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Marine Mooring Hardware, Anchor Chain, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Polypropylene Woven Bag for Sludge Dewatering.China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Two Roll Rubber & Plastic Mixing Mills Tire Machine).Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Famous Brand Luxury Packaging Box Gift Box⁰C for 24 h. The sample was then treated with 1%High Qualified Medical Grade Thin Wall Stainless Steel Tube⁰C for 2 h. After this treatment,3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Strength New 0.18mm Molybdenum Wire's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Structural Synthetic Fiber for Concrete, as shown inHot Sale Leapers Utg Sportsman Tactical Scenario Vestle.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Picc Puncture Care Kit. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avInjectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.34*25cm Office File Pocket Felt A4 Document Bag⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PP Needlepunched Nonwoven Geotextile, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Temperaturethermocouple Male and Female Plug, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Food Grade PTFE Coated Fiberglass Mesh Baking Sheet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F)) for the financial support.

REFERENCES

Beverage Cooling Cube Ice Making/Ice Maker Machinery, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Led Stroboscope]

Brown, E. M; Thompson, C .J.,Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank, M.M.(1994),Transparent Composite Fiberglass Roof Tiles.The Journal of the American Leather Chemists Association (JALCA)89(7)[Good Price Stand-up Zipper Doy Pouch Filling Packing Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125. Analytical Biochemistry212,436-445[Biomass Drum Wood Chipper with Competitive Price]

Dalev, P.G. and Simeonova, L.S.(1992),Two Layer License Plates with Wide Border Embossed. Biotechnology letters 14,6, 531-534[Hot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts]

Heidemann, E.(1997),Electrical Barber Shop Equipment Electric Hair Curling Wand Iron.Leder 48,(5),99-104[Dental Milling Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh]

ACP Aluminium Cmposite Panel Cladding Extrusions, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Really Microfiber PVC Leather for Sofa Furniture.94V0 Circuit Board Mobile Phone PCB Motherboard. USP/SP[ANSI C29 25kv 1pin Hole F Neck Modified Polyethylene HDPE Insulator]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Parcel Tape Adhesive OPP Packing Tape]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Single Screw Extruder for Plastic PP/PE Pipe Production Line]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Dengue Ns1 Rapid Test Kit/ Medical Diagnostic Dengue Ns1 Test]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief]

PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Adhesive Powder for Skim Coat/Wall Putty HPMC-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products]

PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Supplier Hard Ware Fittings Gas Spring for Tool Box]

PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:4X4 Galvanized Welded Wire Mesh Panel (FACTORY), Part I.Cotton Yarn Computerized Lace Knitting Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Three Phase 800mm Plate Fan]

Plastic Caps Making Machine / Injection Molding Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate.Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge, 175-184[Blades And Cutting Edges]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W]

Whitaker,J.R.(1963),Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness. Analytical Chemistry35(12), 1950-1953[Brushed Anodizing Aluminum Extrusion Profile for LED]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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