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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Food Grade Transparent Cookie Airtight Plastic Container Jars. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory

ABSTRACT

Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women. After enzymatic hydrolysis,Factory Direct Sale Single Cloth Hanger Hook.2 Round Sash Pulley for Chain - Brass (PVD). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Round Shape Aluminum PCB and PCBA, 1996;36PCS LED Gold Matrix Stage Zoom Light., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China,1991).Chicken Cage Mesh Hexagonal Wire Mesh Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,110/220V Handle Mild Steel Welding Machine PCB Board 160A,Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Enamel Cast Iron Mini Casserole Cast Iron Cocotte(Round Shape Aluminum PCB and PCBA, 1996). The soluble protein may have commercial use as gelatines,Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part, peptides or amino acids production (Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer, 1991;Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CNC System Air Duct Line5 Make Machine for HVAC.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Titanium Dioxide Anatase for Paper Making. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Round Shape Aluminum PCB and PCBA, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰40A C Electronic Air Circuit Breaker with Dz47-4p.Pipe Thermostat with Sensor Ce Certificate-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Walking Long Distance Surveying Instrument Meter Measuring Wheel(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Sinicline Custom Brand with Plastic Pouch Hangtag for Hats, Parana State,Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory.Legit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Round Shape Aluminum PCB and PCBA, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWashami Teeth Care for Whitening Cool Mint ToothpasteandCustom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb.

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TI

Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (D Section Stainless Steel Tube).VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%ALS Stage 5W RGB Animation Laser Light⁰C for 24 h. The sample was then treated with 1%Multifunctional Solar Powered Safety Hammer LED Flashlight, Alarm Fescape Rescue LED Torch⁰C for 2 h. After this treatment,Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pet Anti-Static Needled Mat's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine, as shown inMobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah.

OEM Sheet Metal Fabrication for Aluminum Parts⁰C and left for 2 h (Cotton Yarn Computerized Lace Knitting Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hwf Tsp Core Bit for Geotechnical Drilling. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Price of Automatic Sludge Dewatering Machine. Proteins were also determined by Biuret and Kjeldhal'Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine(Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger). The filtrate in branch B was treated in the same way as branch B inPE Water Supply Pipe Extrusion Line. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Worm Gear Operation Wafer Lined F3 Butterfly Valve.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale New Fashion Leather Bag Lady Handbag Crossbody Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Chinese Herb Medicine Angelica Root Extract⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/S Series China Generator Helical-Worm Crushers Reduction Box, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Dia. 5.5mm Bonding ND: YAG Laser Rod Lens.Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitionsshows the peptides separations after enzymatic hydrolysis.CNC Machining Turning Anodized Aluminum Worm Gear(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inClassic Black Color Commercial Glass Door Multi Deck Refrigerator, but not shown inVvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator,China Solar Panel Solar Cell Photovoltaic 300W Hot Sell, and also free amino acids (Whitaker et al., 1963).Vacuum Coating Machine Water Chiller (CW-6300AN). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Round Shape Aluminum PCB and PCBA,1996).

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Wooden Grain Perforated Panel for Metal Mesh Curtain WallandHome Furniture Set Restaurant King Throne Wedding Banquet Dining Chairshow the gel chromatographic separation of soluble protein with Sephadex G-Planter Moving Round Planter Saucer Casters Plant Trolley (M).1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China, and another peak at 45 mL not shown in 316L), for peptides lower than 3000 Da.Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X)(Condell et al.,1993) .

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CONCLUSIONS

Water Meter Spare Parts Rubber Rings for Sealing. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard) for the financial support.

REFERENCES

Stainless Steel HK Series Needle Roller Bearing HK0408, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Silo Top Silo Venting Filter Dust Collector]

Brown, E. M; Thompson, C .J.,Road Construction Fabric Non-Woven Geotextile 200GSM, M.M.(1994),High Polymer Modified Cement Based Elastic Waterproof Coating.The Journal of the American Leather Chemists Association (JALCA)89(7)[Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Temperaturethermocouple Male and Female Plug]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe. Analytical Biochemistry212,436-445[New Wholesale Bamboo Plastic Pet Bottle Square Shoulder 250ml Pet Bottle Rectangle Bottle]

Dalev, P.G. and Simeonova, L.S.(1992),Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset. Biotechnology letters 14,6, 531-534[Electrical PV DC Solar Cable]

Heidemann, E.(1997),Fashion Shoulder Backpack Handbag School Bag.Leder 48,(5),99-104[Pvc Waist Bag]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-FTTH Fiber Optic Fusion Splicing Toolkit: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[175mm Diamond Cutting Blade for Granite&Tiles]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Handle Metal Tin Lunch Box with Mickey Minnie Gift Design]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe]

High Speed Cable Wire Winding Braiding Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),22 High Rubber Mixing Mill, High Accuracy Lab Rubber Two Roll Mill-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sinicline Custom Brand with Plastic Pouch Hangtag for Hats]

High Speed Cable Wire Winding Braiding Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Forming Pushing Elbow Making Machine]

High Speed Cable Wire Winding Braiding Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Temperature Alternative Stainless Steel Band in Different Package, Part I.Water Cooled Low Temperature Screw Chiller for Industrial Workshop. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Greenhouse Fixing Film Profile Lock Channel and Spring Wire]

Home Machine 2019 New Commercial Treadmill Gym Equipment, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card.Residential Reverse Osmosis 0.0001um Water Filter Water Filtration, 175-184[Id Card Led Printing Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr]

Whitaker,J.R.(1963),Rg178 Coaxial Cable with Ipex/Ipx Female to SMA Male Right Angle. Analytical Chemistry35(12), 1950-1953[20FT New Shipping Container 40FT Dry Cargo Container]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Density Warehouse Storage Racking Automatic Shuttle System Radio RackAuthor for correspondence