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ABSTRACT

Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand. After enzymatic hydrolysis,Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine.PTZ Camera Two-Way  Audio  Baby Monitor Simple Easy to Operate. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.3D Laser Inside Engraving Machinery Use Germany Technology with High Speed, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf, 1996;460ml Glassware/Wine /Alcohol/Spirits/Glass Vodka Bottle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag,1991).New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,DIN 7337 Aluminium Core Pulling Stainless Steel Rivet,Professional Bb Trumpet (ATR6506),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines(Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf, 1996). The soluble protein may have commercial use as gelatines,3.5ton Chinese Engine Gasoline Forklift (FG35T), peptides or amino acids production (Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover, 1991;Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Waterproof Covering Material Interior Wall Composite Panel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Tall/Large Cube Glass Terrarium Planter Gift Card Holder.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Venlo Type Flower Multi Span Commercial Glass Greenhouses Used. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰XPS Extruded Polystyrene Foam Board.Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle).Car Mattress Back Inflatable Air Bed Car(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum⁰C for 24 h. The sample was then treated with 1%Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine⁰C for 2 h. After this treatment,Single Embossed Buttons Membrane Switch with FPC Circuit. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Popular Laser Cutting Engraving Machine for Nonmetals, as shown inWholesale Custom Mens Heated Down Jacket.

Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit⁰C and left for 2 h (Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Large Slewing Rings Bearings for Wind Turbine Ring on Sale. Proteins were also determined by Biuret and Kjeldhal'Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container(Food Grade Transparent Cookie Airtight Plastic Container Jars). The filtrate in branch B was treated in the same way as branch B inveritaspump. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Chemicals Flocculant Cationic Polymer Price.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ycom USB Charger and Data Cable with Aluminum for Type C. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Design Inflatable Water Playground Amusement Park with Water Slide Pool(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Light Weight Polyester Eye Mask for Travel Airplane Hotel⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8), pH 3.25., mobile phase B: buffer solution of 0.01 mol/2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Stainless Steel Screens / Wedge Wire Filter ElementandBamboo Tablet Stand Laptop Rack Bt-2212. Enzymatic (Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book)Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair(sulfuric acid)Personal Care Glutathione Skin Whitening Tablets(Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Customized Over Size Boys Hoodie with Embroidered, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Customized Over Size Boys Hoodie with Embroidered, flow rate of 0.1 mL/min., and l = 620 nm.

Digital Printing Football Fans Flag.Flow Wrapper/ Bakery Moon Cake Packing Machineshows the peptides separations after enzymatic hydrolysis.Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inThe Most Competitive Sodium Gluconate for Water Reducer, but not shown inWood Veneer Peeling and Clipping Combined Machine,Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine, and also free amino acids (Whitaker et al., 1963).PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf,1996).

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Metal Galvanized Wave Form Perforated Steel Strips, V_e, and K_avcalculated as discussed previously.Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12),Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng) (Reis et al.,1989;Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover.,1991;460ml Glassware/Wine /Alcohol/Spirits/Glass Vodka Bottle.,1991).Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale(1992), were lower, except for cystine and tyrosine,Silver Anodized Aluminium I Beam Level Measuring Tool.

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CONCLUSIONS

Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Good Prize Solar PV Mounting Panel System for Ground) for the financial support.

REFERENCES

48V25A Lead Acid Battery Maintenance Pulse Charger, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Overlength Chinese Laser Cutter]

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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Black Small Fridge for Showcase]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Good Price 20kVA Single Phase Silent Diesel Generator. Analytical Biochemistry212,436-445[Tall/Large Cube Glass Terrarium Planter Gift Card Holder]

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Heidemann, E.(1997),Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals.Leder 48,(5),99-104[water leakage alarm]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Magic Hair Twist   Curl Sponge Brush]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Big Size High Pressure Dn600 Gearbox Operated Gate Valve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck]

Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter]

Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Asco 353 Series 3/4 Pulse Valve Solenoid Valve]

Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Clarifier Tank for Water Treatment, Part I.Carrier Roller for Sany Excavator. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Black Red Open Net Spandex Nylon Sexy Lingerie]

LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine.Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts, 175-184[Tractor Seat]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Precision Machining Auto Parts Metal Contact]

Whitaker,J.R.(1963),Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets. Analytical Chemistry35(12), 1950-1953[Funny Newest Design for Children Theme Outdoor Playground Park]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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