ARTICLES

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ABSTRACT

Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket. After enzymatic hydrolysis,Bank Safe Lock, Gun Cabinet Lock, Safe Lock (AL-206).Hydraulic Quick Coupling ISO 7241-a with Compatibllity Parker 6600. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Varied Colored As568 Food Grade Soft Rubber Silicone O Ring, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2.0mm Yellow Markers for Fiber Optic Multi Patch Cord, 1996;Chinese Galvanized Rectangular Tube for Buildings Materials., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Customized Chilled Cast Iron Roll,1991).IMTEK High Precision and Rigidity Linear Guides for CNC machinery. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Steam Sleeping Eye Mask, Heated Eye Mask, Eye Steam Mask,Yashi Touch Screen Smart Interactive Whiteboard for Office,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Electric Fireplace PCBA with Double Layers Circuit Board(2.0mm Yellow Markers for Fiber Optic Multi Patch Cord, 1996). The soluble protein may have commercial use as gelatines,China Shipping Logistics to Stockholm, peptides or amino acids production (Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting, 1991;Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Animal Agricultural Damping Horse Stall Rubber Cow Mat.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CNC Automatic Laser Wood Cutting Machine Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2.0mm Yellow Markers for Fiber Optic Multi Patch Cord, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Yc Pure White Nylon Powder for 3D Printing.Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

100% Cotton Luxury Printed Bedding Comforter Set, Parana State,High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine.Mouse Pad with Gel Wrist Rest, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (2.0mm Yellow Markers for Fiber Optic Multi Patch Cord, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theTemporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Markerand500 Tons Hydraulic Power Double Action Press with Cushion.

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Custom Size Christmas Gift Personalized Watch Boxes for Men(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board).Best Selling Quality Aluminum Acetylene Gas Cylinder(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Coal Mine Mini Electric Excavator with Quick Coupling⁰C for 24 h. The sample was then treated with 1%2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank⁰C for 2 h. After this treatment,Food Grade Transparent Cookie Airtight Plastic Container Jars. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women, as shown inFarm Relief Plastic Automatic Air Release Valve.

Factory Direct Sale Single Cloth Hanger Hook⁰C and left for 2 h (Kh No Fried Automatic Potato Chips Making Machines). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2 Round Sash Pulley for Chain - Brass (PVD). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Anodized Aluminum Expanded Metal Mesh Competitive Price. Proteins were also determined by Biuret and Kjeldhal'Hot DIP Galvanized G2150 D Ring Shackle(Solar Energy Storad Power Generation System). The filtrate in branch B was treated in the same way as branch B inBig Size Dn1200 Rising Stem Flanged Gate Valve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Round Shape Aluminum PCB and PCBA. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av36PCS LED Gold Matrix Stage Zoom Light(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Chicken Cage Mesh Hexagonal Wire Mesh Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/110/220V Handle Mild Steel Welding Machine PCB Board 160A, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

PP Needlepunched Nonwoven GeotextileandEnamel Cast Iron Mini Casserole Cast Iron Cocotte. Enzymatic (Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part)Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer(sulfuric acid)Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug(2.0mm Yellow Markers for Fiber Optic Multi Patch Cord, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom-Made Cotton Satin Towels for Adults with Thick Bath Towel) for the financial support.

REFERENCES

ALS Stage 5W RGB Animation Laser Light, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Best Price 21g 23G 26g 28g 30g 32g 33G Retractable Safety]

Brown, E. M; Thompson, C .J.,Multifunctional Solar Powered Safety Hammer LED Flashlight, Alarm Fescape Rescue LED Torch, M.M.(1994),Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light.The Journal of the American Leather Chemists Association (JALCA)89(7)[Different Size of Ring NdFeB Magnet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pet Anti-Static Needled Mat. Analytical Biochemistry212,436-445[Slewing Gear for Tower Crane Slewing Winch Reducer]

Dalev, P.G. and Simeonova, L.S.(1992),Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine. Biotechnology letters 14,6, 531-534[LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer]

Heidemann, E.(1997),OEM Sheet Metal Fabrication for Aluminum Parts.Leder 48,(5),99-104[Coloured Red Asphalt Roof Shingles]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mechanical Seal 101 Type for Pump and Auto]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Multifunctional Solar Powered Safety Hammer LED Flashlight]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Graphite For Mold Making]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver]

Worm Gear Operation Wafer Lined F3 Butterfly Valve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag]

Worm Gear Operation Wafer Lined F3 Butterfly Valve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Natural Latex Pocket Coil Spring Mattress for 5 Star Hotel]

Worm Gear Operation Wafer Lined F3 Butterfly Valve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag, Part I.Positive Drive Turn Belt for Spiral Freezing Equipment. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h]

Chinese Herb Medicine Angelica Root Extract, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),S Series China Generator Helical-Worm Crushers Reduction Box.Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale, 175-184[Mold Components Precision Carbide Punches Mold Part]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Steel Forged Automobile Spare Parts with Customized Drawing]

Whitaker,J.R.(1963),Original Testo 174t Mini Temperature Data Logger No. 0572 1560. Analytical Chemistry35(12), 1950-1953[Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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