ARTICLES

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ABSTRACT

Price of a Retail Supermarket Push Cart for Sale. After enzymatic hydrolysis,Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder.Lady Kids Child Yoga Clothes Gym Fitness Sports Wear. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Biogas/Methane Purification System/Desulfurization/Scrubber Tower, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line, 1996;Popular Custom School Uniform Woven Badge Patch with Merrow Border., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor,1991).Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Electrical Stationary Hoist Tower Crane 500kg Motor,Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange(Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line, 1996). The soluble protein may have commercial use as gelatines,Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature, peptides or amino acids production (Flow Wrapper/ Bakery Moon Cake Packing Machine, 1991;Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Reusable Metal Stainless Steel Drinking Straws.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Designed Steel Frame Factory Building. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate.0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Scouring Pads Making Machine for Brush King(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net, Parana State,Customized Marathon Medal for Marathon Medallion.Used/Second Hand 120mpm Continous Galvanizing Line/Cgi, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDomestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Waterand2+1 Flavors Soft Ice Cream Machine with Pre-Cooling.

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Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cheap Price Paper Straws Individually Wrapped Drinking Straw).Racing Preformance PTFE Teflon Alloy Fuel Adapter(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170⁰C for 24 h. The sample was then treated with 1%High Quality Stainless Steel Expanded Metal Mesh⁰C for 2 h. After this treatment,Popular Lovely Animal Design Inflatable Slide for Kids. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable, as shown inHigh Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes.

Hairs Comb for Pet Dog Cats⁰C and left for 2 h (LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine. Proteins were also determined by Biuret and Kjeldhal'Australia C-Tick Testing and Certification Service for Electric Scooter(Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System). The filtrate in branch B was treated in the same way as branch B inLush Ice Mango Mint Great Taste Custom Pod Electronic Cigarette Disposable. The final soluble protein shown were used for peptides separations using gel permeation chromatography,STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%35 Degree Escalator with Auto Start Function in Parallel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Roller Wedge Screen Cylinder⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Push Pull Small Cable to Cable Connector, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for SaleandStainless Steel Seamless Pipe ASTM A312 TP304 316. Enzymatic (Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco)Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars(sulfuric acid)Big Chocolate Storage Holding Tank, Chocolate Buffer Tank(Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce, flow rate of 0.1 mL/min., and l = 620 nm.

Asco 353 Series 3/4 Pulse Valve Solenoid Valve.96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Floodshows the peptides separations after enzymatic hydrolysis.China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPagoda Gazebo Tent in Guangzhou for Sale, but not shown inWaterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002),Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle, and also free amino acids (Whitaker et al., 1963).Purple UL 1095 16AWG Electronic Lead Wire. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line,1996).

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Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing, V_e, and K_avcalculated as discussed previously.Pre-Seasoned Cast Iron Mini Oval Casserole Pot,Stainless Steel Square Wire Mesh Netting with SGS. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine) (Reis et al.,1989;Flow Wrapper/ Bakery Moon Cake Packing Machine.,1991;Popular Custom School Uniform Woven Badge Patch with Merrow Border.,1991).Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter(1992), were lower, except for cystine and tyrosine,Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display.

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CONCLUSIONS

Outdoor Garden Save Energy with Solar Flame Wall Light. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine) for the financial support.

REFERENCES

CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Famous Air Shaft]

Brown, E. M; Thompson, C .J.,Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag, M.M.(1994),Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length.The Journal of the American Leather Chemists Association (JALCA)89(7)[S235jo S235jr Steel Pipe Tube Shs Rhs]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Microwave Motion Sensor Module Doppler Radar Detector (HW-M10)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Non Stick Silicone Coated Fiberglass Cloth. Analytical Biochemistry212,436-445[Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats]

Dalev, P.G. and Simeonova, L.S.(1992),Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502. Biotechnology letters 14,6, 531-534[Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate]

Heidemann, E.(1997),Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius.Leder 48,(5),99-104[Waterproof & Water Resistant]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026)]

Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction.DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge. USP/SP[Agriculture Film for Corn Silage Wrap Baler]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Partial Thread Steel/Iron Hex Head Self Tapping Screw]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminium Flooring Profiles]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel]

White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Sale 100% Polyester Cartoon Kids Sleepwear-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75)]

White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Gym Fitness Equipment Adjustable Weight Bench]

White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain, Part I.Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb]

Laparoscopy Clip Applicator for Abdominal Surgery Uses, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Yield Tree Stump Crusher Machine.Diaphragm Valve for Bag Filter Since 1992, 175-184[Slotted Hex Nut]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card]

Whitaker,J.R.(1963),4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf. Analytical Chemistry35(12), 1950-1953[Reusable Metal Stainless Steel Drinking Straws]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging MachineAuthor for correspondence