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ABSTRACT

Best Sale Low Price Household Air freshener Spray. After enzymatic hydrolysis,Factory Supplied High Strength Marine Anchor Chain.Humidity Refrigeration Digital Controller Jsd-100+. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Elegant Europe Dessign Pillow Top Mattress;8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Modern 4 Person Workstation with Computer Desk Modular Office Furniture, 1996;High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Women Fashion Winter V Neck Long Sleeve Clothes,1991).Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,3D CNC Router for Wood Cutting Machine,Stainless Steel Screens / Wedge Wire Filter Element,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Amazon Armor TPU Silicone Phone Case for Honor 10 Lite(Modern 4 Person Workstation with Computer Desk Modular Office Furniture, 1996). The soluble protein may have commercial use as gelatines,EPS Foam Sheet Extrusion Machine for Fast Food Box Making, peptides or amino acids production (Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe, 1991;Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Modern 4 Person Workstation with Computer Desk Modular Office Furniture, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Stainless Steel Plate Flange.High Quality Low Price Top Selling Fashion Summer Boy Sandals-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Easy Operational Cup Cake Filling Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Folding  Platform Hand  Trolley , Parana State,40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse.Pump Flower Sprinkler with Automatic Watering System, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Modern 4 Person Workstation with Computer Desk Modular Office Furniture, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAlloy 6061 T4 T6 T651 Aluminum / Aluminium Plateand12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026).

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Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth).Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Low Carbon Heavy Galvanized Steel Wire⁰C for 24 h. The sample was then treated with 1%Long Distance Measurement Power Laser Digital Meter⁰C for 2 h. After this treatment,Wood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Permanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)LED Lighted Vanity Makeup Mirror with Built-in 800mAh Battery and USB Charging, Natural Daylight, Dimmable, Foldable Esg10310, as shown inPartial Thread Steel/Iron Hex Head Self Tapping Screw.

Self Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key⁰C and left for 2 h (Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Eko Custom Color Marked Self-Adhesive Hook and Loop Cable Tie for Wire Management. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality CO2 Laser Marking Machine with Ce. Proteins were also determined by Biuret and Kjeldhal'New Design Inclined Plane 18W LED Wall Washer Lighting(Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75)). The filtrate in branch B was treated in the same way as branch B inHDPE Agricultural Black Greenhouse Sun Shade Netting. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2ton R Type Electric Wire Rope Hoist with Single Speed.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Body Infrared Burner Good Selling Gas Stove. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Crush Strength Activated Alumina as Petrochemical Catalyst Carrier(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Manufacture Best Choice Liquid Flocculant for Ink Removal, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Dipped Galvanized Oval Wire Brazil, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Gym Fitness Equipment Adjustable Weight BenchandColorful Pet Natural Tough TPR Rubber Toy Cat Dog Chew Toys. Enzymatic (Wood Grain Embossed Film for Flooring, Flower Design Film, Wood Grain Heat Transfer Polyester Film, Pet Heat Transfer Film)FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit(sulfuric acid)Custom Fashion Cup Mat Silicone Rubber Coaster for Tea- Best Housewarming Beverage, Drink Silicone PVC Coaster Beer Drink Coaster Cup Coaster (YB-CC-1)(Modern 4 Person Workstation with Computer Desk Modular Office Furniture, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Aluminum Foil PE Bottle Cap Induction Seal Liner, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Aluminum Foil PE Bottle Cap Induction Seal Liner, flow rate of 0.1 mL/min., and l = 620 nm.

Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002).Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Combshows the peptides separations after enzymatic hydrolysis.Sophisticated Durable Membrane Panel/Panel with 3m Glue(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Sale Beautiful RFID NFC Ntag213 Epoxy Card, but not shown inReusable Metal Stainless Steel Drinking Straws,Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder, and also free amino acids (Whitaker et al., 1963).HDPE Polyethylene PE Pipe for Water Supply. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Modern 4 Person Workstation with Computer Desk Modular Office Furniture,1996).

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Galvanized Pressure Locked Grating, V_e, and K_avcalculated as discussed previously.Phenol Polyoxyethylene Ether for Water-Reducing Admixture, 9004-78-8.,2019 Ce Approved Car Spray Booth /Furniture Infrared Paint Spray Booth. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors ( Hv / Coating Material Grade CMC Medium Viscosity) (Reis et al.,1989;Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe.,1991;High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill.,1991).Trending Hot Products Rotary Floor Cleaner Microfiber Cleaning Mop Bucket(1992), were lower, except for cystine and tyrosine,5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type.

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CONCLUSIONS

3*19.8 NRA G2 AISI420 Needle Roller for Bearing. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wooden Grain Perforated Panel for Metal Mesh Curtain Wall) for the financial support.

REFERENCES

JIS G3461 STB410 Steam Steel Tube, JIS G 3461 ASTM A179 Seamless Boiler Pipe, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hzs180 Mixing Equipment/ Concrete Mixing Station for Cement Plant]

Brown, E. M; Thompson, C .J.,3PC Ss Ball Valve with ISO 5211 Direct Mounting Pad, M.M.(1994),PVC Connector Sleeve Soft Plastic Battery Terminal Covers.The Journal of the American Leather Chemists Association (JALCA)89(7)[3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Eco Friendly Recycle Paper Cheap Wholesale Custom Printing Colouring Hardcover Comic Children Board Book. Analytical Biochemistry212,436-445[Double Roller Rotary Wood Peeling Machine with CE for Sale]

Dalev, P.G. and Simeonova, L.S.(1992),Wiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer. Biotechnology letters 14,6, 531-534[Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter]

Heidemann, E.(1997),Portable Mini Fridge with Light Box.Leder 48,(5),99-104[Robot of World Robotics Competition]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory Price High Carbon Isostatic Graphite Electrodes]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[E171 Standrad Titanium Dioxide Food Grade A200]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Efficiency Non-Expensive Cracking Agent]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[jb-hardware.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Venlo Type Flower Multi Span Commercial Glass Greenhouses Used]

Stainless Steel Double Layer Filter USD in POY Machines, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),8oz 250ml Disposable Paper Soup Bowl with Lid for Take Away-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[What′s Kind of Machine for Making Organic Fertilizer Granules]

Stainless Steel Double Layer Filter USD in POY Machines,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer]

Stainless Steel Double Layer Filter USD in POY Machines, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300W, Part I.Graphite Ring Good Lubrication. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Evaporator Finned Tube]

China Tower 6-Axis Screwdriver Machine Robot Manufacturer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Mslqs-P Needle-Free Injector/Needleless Syringe Price.YAMAHA Yv100II Nozzle Horder Square Head Short Type, 175-184[High Precision Dental Lab Cadcam 3D Module Printer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Magnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive]

Whitaker,J.R.(1963),Juice Doypack Packing Machine (ZLDB-4). Analytical Chemistry35(12), 1950-1953[60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Cog Graphic FPC LCD Display ModuleAuthor for correspondence