ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Dxz Limit Switch Tower Crane Spare Parts. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock

ABSTRACT

High-End Business Magnet Leather Ring Binder Notebook. After enzymatic hydrolysis,Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops.New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Eyes- Caring LED Night Light for Nursing Reading with Soft Light;China Bearing with Housing Bearing / Pillow Block Bearing; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (The Most Competitive Sodium Gluconate for Water Reducer, 1996;Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Electric Magic Hot Hair Styling Curling Iron with Brushes,1991).Melt Pressure Transducer (PT133). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe,Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine(The Most Competitive Sodium Gluconate for Water Reducer, 1996). The soluble protein may have commercial use as gelatines,Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game, peptides or amino acids production (Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662, 1991;Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Durable Aluminum Flag Pole with Dome Top Tangle Free Finial.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Customized Marathon Medal for Marathon Medallion. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (The Most Competitive Sodium Gluconate for Water Reducer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Price of a Retail Supermarket Push Cart for Sale.Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Lady Kids Child Yoga Clothes Gym Fitness Sports Wear(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Popular Custom School Uniform Woven Badge Patch with Merrow Border, Parana State,OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock.1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (The Most Competitive Sodium Gluconate for Water Reducer, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-HookandGood Strength GRP Cooling Towers for Mechanical Cooling.

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Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps).Electrical Stationary Hoist Tower Crane 500kg Motor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage⁰C for 24 h. The sample was then treated with 1%Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange⁰C for 2 h. After this treatment,Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Flow Wrapper/ Bakery Moon Cake Packing Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine, as shown inDxz Limit Switch Tower Crane Spare Parts.

Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh⁰C and left for 2 h (Automatic Chewing Dog Food Snack Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Reusable Metal Stainless Steel Drinking Straws. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Designed Steel Frame Factory Building. Proteins were also determined by Biuret and Kjeldhal'Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate(Orange Monofilament Fishing Tackle Nylon Fish Net). The filtrate in branch B was treated in the same way as branch B inCf8 Cf8m 904l Butterfly Valve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Scouring Pads Making Machine for Brush King. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIdeabond Stone Texture ACP Aluminum Composite Panel for Outdoor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Popular Lovely Animal Design Inflatable Slide for Kids.Good Quality Pillow for Pregnant Wome Body Pregnant Pillow Caseshows the peptides separations after enzymatic hydrolysis.Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3), but not shown inHigh Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing,GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable, and also free amino acids (Whitaker et al., 1963).Hairs Comb for Pet Dog Cats. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (The Most Competitive Sodium Gluconate for Water Reducer,1996).

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Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with EarloopsandBlack Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipeshow the gel chromatographic separation of soluble protein with Sephadex G-Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall.Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine, and another peak at 45 mL not shown inNew Condition Hot Sale Type Air Cooled Ice Cube Maker, for peptides lower than 3000 Da.Australia C-Tick Testing and Certification Service for Electric Scooter(Condell et al.,1993) .

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STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,, V_e, and K_avcalculated as discussed previously.35 Degree Escalator with Auto Start Function in Parallel,Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb) (Reis et al.,1989;Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662.,1991;Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles.,1991).Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h(1992), were lower, except for cystine and tyrosine,Roller Wedge Screen Cylinder.

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CONCLUSIONS

Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Biogas/Methane Purification System/Desulfurization/Scrubber Tower) for the financial support.

REFERENCES

Push Pull Small Cable to Cable Connector, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Shower Floor Drainage]

Brown, E. M; Thompson, C .J.,Stainless Steel Seamless Pipe ASTM A312 TP304 316, M.M.(1994),Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cotton Yarn Computerized Lace Knitting Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars. Analytical Biochemistry212,436-445[Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger]

Dalev, P.G. and Simeonova, L.S.(1992),Big Chocolate Storage Holding Tank, Chocolate Buffer Tank. Biotechnology letters 14,6, 531-534[Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking]

Heidemann, E.(1997),Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce.Leder 48,(5),99-104[Industry Grade Ferrous Sulfate Dried]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions]

125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Asco 353 Series 3/4 Pulse Valve Solenoid Valve.China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material. USP/SP[Classic Black Color Commercial Glass Door Multi Deck Refrigerator]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Male and Female Automotive Connector]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wooden Grain Perforated Panel for Metal Mesh Curtain Wall]

Purple UL 1095 16AWG Electronic Lead Wire, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Brushed Anodizing Aluminum Extrusion Profile for LED-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair]

Purple UL 1095 16AWG Electronic Lead Wire,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ 316L)]

Purple UL 1095 16AWG Electronic Lead Wire, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps, Part I.S235jo S235jr Steel Pipe Tube Shs Rhs. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant]

Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pre-Seasoned Cast Iron Mini Oval Casserole Pot.Stainless Steel Square Wire Mesh Netting with SGS, 175-184[Gym Equipment Portable Electric Hydraulic Basketball Hoop]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[22 High Rubber Mixing Mill]

Whitaker,J.R.(1963),Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter. Analytical Chemistry35(12), 1950-1953[Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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