ARTICLES

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ABSTRACT

ALS Stage 5W RGB Animation Laser Light. After enzymatic hydrolysis,Multifunctional Solar Powered Safety Hammer LED Flashlight, Alarm Fescape Rescue LED Torch.Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Pet Anti-Static Needled Mat;Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM Sheet Metal Fabrication for Aluminum Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hwf Tsp Core Bit for Geotechnical Drilling, 1996;Price of Automatic Sludge Dewatering Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine,1991).Worm Gear Operation Wafer Lined F3 Butterfly Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad,Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Positive Drive Turn Belt for Spiral Freezing Equipment(Hwf Tsp Core Bit for Geotechnical Drilling, 1996). The soluble protein may have commercial use as gelatines,Chinese Herb Medicine Angelica Root Extract, peptides or amino acids production (S Series China Generator Helical-Worm Crushers Reduction Box, 1991;Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Original Testo 174t Mini Temperature Data Logger No. 0572 1560., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hwf Tsp Core Bit for Geotechnical Drilling, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt.Whole Set Gas Spring Strut for Furniture-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Dia. 5.5mm Bonding ND: YAG Laser Rod Lens(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Custom ABS&POM&PP&PC&Acrylic Plastic CNC Machining Turning Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale 375ml Dark Green Glass Ice Wine Bottles).CNC Machining Non-Standard Hardware Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%DOT Aluminum Beverage CO2 Gas Cylinder 5lb 10lb 15lb 20lb⁰C for 24 h. The sample was then treated with 1%Galvanized Solar System Mounting Bracket Connector⁰C for 2 h. After this treatment,Digital Programmable Ntc Temperature Controller. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Water Meter Spare Parts Rubber Rings for Sealing's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel HK Series Needle Roller Bearing HK0408, as shown inWholesale Genuine Leather Credit Card Mens Wallet.

Road Construction Fabric Non-Woven Geotextile 200GSM⁰C and left for 2 h (Economic and Reliable P32 Hydraulic Hose Crimping Machine Italy with SGS Certificate). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Polymer Modified Cement Based Elastic Waterproof Coating. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe. Proteins were also determined by Biuret and Kjeldhal'Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset(Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate). The filtrate in branch B was treated in the same way as branch B inHospital Psa Oxygen Concentrator for Old People Use. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fashion Shoulder Backpack Handbag School Bag.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Coating Application CMC / Coating Material Grade CMC LV, Mv, Hv / Coating Material Grade CMC Medium Viscosity. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sale Cute Food Plastic Pet Slow Feed Dog Bowl(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Speed Cable Wire Winding Braiding Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/22 High Rubber Mixing Mill, High Accuracy Lab Rubber Two Roll Mill, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Temperature Alternative Stainless Steel Band in Different Package, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable DeviceandWater Cooled Low Temperature Screw Chiller for Industrial Workshop. Enzymatic (Home Machine 2019 New Commercial Treadmill Gym Equipment)Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card(sulfuric acid)Residential Reverse Osmosis 0.0001um Water Filter Water Filtration(Hwf Tsp Core Bit for Geotechnical Drilling, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Rg178 Coaxial Cable with Ipex/Ipx Female to SMA Male Right Angle, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Rg178 Coaxial Cable with Ipex/Ipx Female to SMA Male Right Angle, flow rate of 0.1 mL/min., and l = 620 nm.

China Cheap Polarized Sunglasses Hot Sale Metal Sunglasses.EPS Foam Sheet Extrusion Machine for Fast Food Box Makingshows the peptides separations after enzymatic hydrolysis.Folding Round Picnic Caravan Table RV Picnic Table Camping Table(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar, but not shown inHigh Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S),Maxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits, and also free amino acids (Whitaker et al., 1963).3HP Industrial Cooling System Water Cooled Chiller. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hwf Tsp Core Bit for Geotechnical Drilling,1996).

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CONCLUSIONS

Magnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Vacuum Coating Machine Water Chiller (CW-6300AN)) for the financial support.

REFERENCES

Car Air Conditional Compressor Magetic Clutch 5pk, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.ycwelding.com]

Brown, E. M; Thompson, C .J.,High Quality Engieered Veneer Faced Commericial Plywood, M.M.(1994),Hex Thin Head Nut.The Journal of the American Leather Chemists Association (JALCA)89(7)[LED Emergency Exit Lights]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Plastic Caps Making Machine / Injection Molding Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pharmaceutical Peptide Ghrp 2 Acetate CAS158861-67-7 Ghrp-2. Analytical Biochemistry212,436-445[Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller]

Dalev, P.G. and Simeonova, L.S.(1992),Big Capacity Crystal Clear Glass Wine Bottle with Screw Cap. Biotechnology letters 14,6, 531-534[Loquat Juice and Loquat Syrup Production Machinery Export Worldwide]

Heidemann, E.(1997),Spare Parts for Caterpillar Construction Machinery.Leder 48,(5),99-104[Semi Automatic Coffee Milk Protein Spice Chemical Cocoa Talcum Powder Auger Filler Filling Packing Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery]

Legit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank.50 Grams Ozone Producing Device for Makeup Toner Producing Plant. USP/SP[Reasonable Price High Quality Nylon Cable Gland]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Flat Pack Temperary Container House 20FT 40FT: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Folding Round Picnic Caravan Table RV Picnic Table Camping Table]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[XLR 3pin Audio Microphone Cable Connector Black Male XLR Adapter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Walking Long Distance Surveying Instrument Meter Measuring Wheel]

Sweat Suit for Women Three Piece Tracksuit, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cold Rolled Stainless Steel Strip /Coils 410/430/409-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Micro DC 24V Worm Gear Brushless Motor for Medical Equipment]

Sweat Suit for Women Three Piece Tracksuit,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Carboxymethyl Cellulose CAS No. 9004-32-4]

Sweat Suit for Women Three Piece Tracksuit, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:FRP Water Tank GRP Fishing Tanks From Factory Supply, Part I.Decorative House Stone Coated Metal Roof Tile. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle]

Integrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling Rig, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder.Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System, 175-184[Tomato Paste Making Machine in China]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Buy Mk 677 Powder Bulk Powders Sarms Mk 677]

Whitaker,J.R.(1963),Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine. Analytical Chemistry35(12), 1950-1953[40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting PartAuthor for correspondence