ARTICLES

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PVC Chain Link FenceEdivaldo E.Garcia^I; Cláudia C.M.Sofa Materials PU Leather with Flock Backing^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{Magnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Three Phase 800mm Plate Fan. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,ASTM Approved Popular Indoor Trampoline of Park

ABSTRACT

Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building. After enzymatic hydrolysis,Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light.Wholesale Genuine Leather Credit Card Mens Wallet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Yitai Die Making 13mm Beech Rotary Plywood;Gear Rolling Machine Zh28/25 Spline Hobbing Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale, 1996;Partial Thread Steel/Iron Hex Head Self Tapping Screw., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Flat Car Sun Roof Tent Awning,1991).Classic Black Color Commercial Glass Door Multi Deck Refrigerator. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits,12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pneumatic Control Shuttle Valve NPT Thread(Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale, 1996). The soluble protein may have commercial use as gelatines,Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing, peptides or amino acids production (OEM 15PCS Electric Face Makeup Brush with Ce Certificate, 1991;New Styles Fashion Erotic Women Underwear Sexy Lingerie, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stand Hot and Cold Normal Water Dispenser with Refrigerator.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Construction Welding Rods Electrode E6013 E7018 E7016. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process.Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Lydite Fence Reel Reel with Extension Arms for Farm Fencing(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor, Parana State,ASTM Approved Popular Indoor Trampoline of Park.Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the60W All-in-One PIR Sensor Die-Aluminum Solar Street Light PriceandCog Graphic FPC LCD Display Module.

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14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask).Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800⁰C for 24 h. The sample was then treated with 1%Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope⁰C for 2 h. After this treatment,High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Aluminum Bright Polished Mirror Sheet, as shown inCAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc.

Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open)⁰C and left for 2 h (Eyes- Caring LED Night Light for Nursing Reading with Soft Light). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Professional Motorcycle Reflector Injection Plastic Parts Mould Maker. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W. Proteins were also determined by Biuret and Kjeldhal'Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China(24 Volt Fire Alarm Strobe Light Horn Strobe Sounder). The filtrate in branch B was treated in the same way as branch B inGood Quality Irregular Bottle Shape Plastic Bag with Printed Logo for Snack Candy Food Packaging. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Customized Souvenirs Tin Metal Number & Name Plate for Car. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAC/DC Pure Sine Wave Inverters for PV Solar System off Grid System(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Pond Self Priming Booster Pressure Water Pump with Check Valve⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Banded Type Tee Used on Oil, pH 3.25., mobile phase B: buffer solution of 0.01 mol/20FT New Shipping Container 40FT Dry Cargo Container, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

YAMAHA Yv100II Nozzle Horder Square Head Short TypeandHigh Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover. Enzymatic (Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone)High Pressure Braided Rubber Oil Hose(sulfuric acid)Monochrome Graphic Industrial Control LCD Display Module 128X64(Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%SMD LED Light-Operated USB Rechargeble Flashlight, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%SMD LED Light-Operated USB Rechargeble Flashlight, flow rate of 0.1 mL/min., and l = 620 nm.

Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden.Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panelshows the peptides separations after enzymatic hydrolysis.1jo4 Hydraulic Adaptors for Hose Fittings(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCorrugated Board Production Line, but not shown inT90 High Power Latching Relay Mini Power Relay,Chinese 1325 Ce Certificated CNC Engraving Router for Sale, and also free amino acids (Whitaker et al., 1963).Oil Heating Reactor. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale,1996).

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Safe Durable Construction Scaffolding Plank for BuildingandFactory Manufacturers Prices Melamine Faced Chipboard/Particle Boardshow the gel chromatographic separation of soluble protein with Sephadex G-Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator.6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring, and another peak at 45 mL not shown inSynthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117), for peptides lower than 3000 Da.All Kinds of Color Fashion Simple Stone Pendant Design Necklace(Condell et al.,1993) .

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Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve, V_e, and K_avcalculated as discussed previously.48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System,Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery) (Reis et al.,1989;OEM 15PCS Electric Face Makeup Brush with Ce Certificate.,1991;Partial Thread Steel/Iron Hex Head Self Tapping Screw.,1991).Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting(1992), were lower, except for cystine and tyrosine,Toilet Roll Tissue Wrapping Machine with Auto - Counting.

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CONCLUSIONS

Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules) for the financial support.

REFERENCES

Orange Monofilament Fishing Tackle Nylon Fish Net, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Stainless Steel Quick Open Hose Clamps SUS304 Hardware Parts]

Brown, E. M; Thompson, C .J.,Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002), M.M.(1994),By6-8-200-L Automatic Doypack Premade Pouch Packing Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[ADC12 Aluminum Die Casting Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Safest Baby Healthcare and Grooming Kit for Baby Gift]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Diameter 1300mm Lifting Electro Magnet for Cast Iron. Analytical Biochemistry212,436-445[Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip]

Dalev, P.G. and Simeonova, L.S.(1992),High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%. Biotechnology letters 14,6, 531-534[Original Testo 174t Mini Temperature Data Logger No. 0572 1560]

Heidemann, E.(1997),Evaporator Finned Tube.Leder 48,(5),99-104[High Quality Medical/ICU Ventilator Lh8600 for Operation and Rehabilitation]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Vitamin D3 Fat-Solubility Water-Solubility Feed Additive]

Sofa Materials PU Leather with Flock Backing, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear.Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine. USP/SP[Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-100%Polyester Spandex Hotel Chair Cover (DPH7022): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[800 * 480 Lcd]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture]

Factory Supply Perforated Metal Mesh, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CNC Turning Center, CNC Horizontal Turning Milling Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849]

Factory Supply Perforated Metal Mesh,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[100W 120W 150W Outdoor Solar LED Street Lighting]

Factory Supply Perforated Metal Mesh, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16, Part I.Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PAC /Poly Aluminium Chloride for Water Treatment]

Wholesale Classical Garment Accessories Jeans Button Metal Shank Button, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Vitamin D3 Fat-Solubility Water-Solubility Feed Additive.Factory Wholesale Zinc Plated Carbon Steel Hex Nut, 175-184[Screen Stainless Steel]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Galvanized Roller Shutter Window Torsion Springs]

Whitaker,J.R.(1963),Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern. Analytical Chemistry35(12), 1950-1953[Italy Design Good Quality Hydraulic Motorcycle Lift Table]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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