ARTICLES

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ABSTRACT

Paper Sticky Lint Remover Roller/Pet Hair Roller. After enzymatic hydrolysis,Safe Durable Scaffolding Trapdoor Plank for Construction.China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube, 1996;Best Quality Bending Metal Mould with All Kinds Metal Materials., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus,1991).Milling Precision Stainless Steel CNC Machinery Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails,Good Price Stand-up Zipper Doy Pouch Filling Packing Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21(Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube, 1996). The soluble protein may have commercial use as gelatines,Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10, peptides or amino acids production (360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1), 1991;Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Food Machine Cube Ice Machine for Commercial Use., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Christmas Decoration Rose Flower LED Light String.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-D Section Stainless Steel Tube. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Mint Flavor 50ml Home Foam Toothpaste.125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Dxz Limit Switch Tower Crane Spare Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent).The Most Competitive Sodium Gluconate for Water Reducer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles⁰C for 24 h. The sample was then treated with 1%Electric Magic Hot Hair Styling Curling Iron with Brushes⁰C for 2 h. After this treatment,Melt Pressure Transducer (PT133). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag, as shown inNew Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Durable Aluminum Flag Pole with Dome Top Tangle Free Finial. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustomized Marathon Medal for Marathon Medallion(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Lady Kids Child Yoga Clothes Gym Fitness Sports Wear, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hotel Steel Digital Room Safes with Large LED Display, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Scouring Pads Making Machine for Brush King. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops) for the financial support.

REFERENCES

Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ce Air-source Heat Pump Water Tank]

Brown, E. M; Thompson, C .J.,Glass Mix Stone Mosaic Tiles, M.M.(1994),Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank. Analytical Biochemistry212,436-445[Price of a Retail Supermarket Push Cart for Sale]

Dalev, P.G. and Simeonova, L.S.(1992),Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net. Biotechnology letters 14,6, 531-534[Enamel Cast Iron Mini Casserole Cast Iron Cocotte]

Heidemann, E.(1997),Used/Second Hand 120mpm Continous Galvanizing Line/Cgi.Leder 48,(5),99-104[Disposable Drip Tip]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts]

High Precision Machining Auto Parts Metal Contact, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie.Racing Preformance PTFE Teflon Alloy Fuel Adapter. USP/SP[Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Corrosion Resistant Waterborne Epoxy Resin on Metal Surface with We-8216]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hand Corn Seeder Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process]

High Quality Stainless Steel Expanded Metal Mesh, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Popular Lovely Animal Design Inflatable Slide for Kids-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662]

High Quality Stainless Steel Expanded Metal Mesh,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ API 5L Spec 5L Steel Pipe]

High Quality Stainless Steel Expanded Metal Mesh, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware, Part I.GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Screen Printing Materials and Equipments Screen Printing Frames]

Hairs Comb for Pet Dog Cats, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall.Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine, 175-184[Ore Mining Plant Vibrating/ Coal Hopper Feeder]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Women Fashion Winter V Neck Long Sleeve Clothes]

Whitaker,J.R.(1963),Australia C-Tick Testing and Certification Service for Electric Scooter. Analytical Chemistry35(12), 1950-1953[Black Phosphated Bugle Head Csk Head Drywall Screw]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Vinyl Glove in Work & Labor GloveAuthor for correspondence