ARTICLES

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ABSTRACT

Really Microfiber PVC Leather for Sofa Furniture. After enzymatic hydrolysis,94V0 Circuit Board Mobile Phone PCB Motherboard.Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve;Adhesive Powder for Skim Coat/Wall Putty HPMC; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.4X4 Galvanized Welded Wire Mesh Panel (FACTORY), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cotton Yarn Computerized Lace Knitting Machine, 1996;Plastic Caps Making Machine / Injection Molding Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate,1991).Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness,LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Safest Baby Healthcare and Grooming Kit for Baby Gift(Cotton Yarn Computerized Lace Knitting Machine, 1996). The soluble protein may have commercial use as gelatines,Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby, peptides or amino acids production (Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro, 1991;Micro DC 24V Worm Gear Brushless Motor for Medical Equipment, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1.6 Ton Hand Lifting Tool Lever Chain Block., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CNC Machining Service High Precision CNC Mechanical Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cotton Yarn Computerized Lace Knitting Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker.Printed 100% Polyester Tcheap Wholesale Blankets-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Food Grade Material 304/316 Double Diaphragm Pumps(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Widely Application Latest Designed Coffee Capsule Filling Packing Machine, Parana State,Electrical Barber Shop Equipment Electric Hair Curling Wand Iron.Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Cotton Yarn Computerized Lace Knitting Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFull Computer Plain Socks Knitting Machine with High QualityandWholesale Pastoral Style Rattan Woven Decorative Storage Box Basket.

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18V Lithium Battery Powered Cordless Drill(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (PRO Audio Loudspeaker PA Speaker System Line Array Speaker).High Density Rock Wool Wall Board with Thermal Insulation Performance(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh⁰C for 24 h. The sample was then treated with 1%Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask⁰C for 2 h. After this treatment,China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China PAC Water Flocculating Agent for Water Treatment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory Price High Carbon Isostatic Graphite Electrodes, as shown inAutomatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Black Phosphated Bugle Head Csk Head Drywall Screw. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLiquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/6 Inch Plastic Breathing Cap for The IBC Valve, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608andWashami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging. Enzymatic (Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree)OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part(sulfuric acid)Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable(Cotton Yarn Computerized Lace Knitting Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars, flow rate of 0.1 mL/min., and l = 620 nm.

Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer.Groove Type Wood Log Tree Peeling and Debarking Machineshows the peptides separations after enzymatic hydrolysis.Freshwater Pearl Butterfly Flower Bride Hairpin(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHex Thin Head Nut, but not shown inModern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture,Air Bubble Insulation Thermal Building Insulation Material, and also free amino acids (Whitaker et al., 1963).LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Cotton Yarn Computerized Lace Knitting Machine,1996).

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Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring PadandTwilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheetshow the gel chromatographic separation of soluble protein with Sephadex G-Rechargeable Battery Operated Cooling Folding Electric Fan.High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve, and another peak at 45 mL not shown inNn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone, for peptides lower than 3000 Da.21.5′′ Wall Mount Display Capacitive Touch Monitor(Condell et al.,1993) .

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CONCLUSIONS

Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Power From Han′s GS 2000W Laser Cutter) for the financial support.

REFERENCES

Zircon Butterfly Open Ring Diamond Fashion Woemn Rings, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Cr12MOV Stainless Steel Plate&Sheet for Thread Rolling Die]

Brown, E. M; Thompson, C .J.,High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation, M.M.(1994),Spare Parts Custom Injection Plastic Moulding Manufacturer.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Industrial Grade Anhydrous Sodium Sulfate]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Safe Lock (AL-206)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill. Analytical Biochemistry212,436-445[4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf]

Dalev, P.G. and Simeonova, L.S.(1992),96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood. Biotechnology letters 14,6, 531-534[Low Carbon Heavy Galvanized Steel Wire]

Heidemann, E.(1997),Kingdomway Brand Vitamin a 1000.Leder 48,(5),99-104[Sintra Forex Board Forex Sheet 3mm Foam PVC Board]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[50Hz to 60Hz Frequency Inverter for Water Pump]

Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine.High Speed Roll Plastic T-Shirt Bag Making Machine. USP/SP[Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hair Styles]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine]

Wholesale Black Galvanized Head Stainless Steel Screw, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Structural Synthetic Fiber for Concrete]

Wholesale Black Galvanized Head Stainless Steel Screw,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Brand New Dnv 2.7-1 Standard 20FT Half Height Offshore Container]

Wholesale Black Galvanized Head Stainless Steel Screw, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:45*60cm Anti-Fatigue ESD Rubber Floor Mat, Part I.Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill]

Vinyl Glove in Work & Labor Glove, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Low Energy Needed Compact Flour Mill Plant for Milling Wheat.Nylon Weatherproof PA6 Strain Relief Cable Gland, 175-184[Drop Fiber Optic Cable]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[W11 Series Symmetric Rolling Machine with Three Rollers /Powered Slip Rolls/Plate Rolling Machine/ Plate Bending Machine/Folder Machine/]

Whitaker,J.R.(1963),Steel Roller Embossed Paper 2019 New Design. Analytical Chemistry35(12), 1950-1953[Soft Baby Suites Dinosaure Pijamas Kigurumi]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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