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ABSTRACT

Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger. After enzymatic hydrolysis,PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle.Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Speed Copper Wire Braiding Making Machine;Safe Durable Construction Scaffolding Plank for Building; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Different Size of Ring NdFeB Magnet, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine, 1996;High Quality Welding Structure., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Carboxymethyl Cellulose CAS No. 9004-32-4,1991).Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip,150kg Per Hour Frozen French Fries Production Line,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Professional LED Display Ceramic Hair Curling Iron(Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine, 1996). The soluble protein may have commercial use as gelatines,CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts, peptides or amino acids production (China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer, 1991;Vulcanizing Machine /Rubber Tiles Vulcanzing Press, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Omni Newest Manager Desk Office Smart Office Table Office Staff Table. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap.30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules).Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%200W UFO LED High Bay Light Fixture for Interior Exterior Applications⁰C for 24 h. The sample was then treated with 1%China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2)⁰C for 2 h. After this treatment,13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Bathroom False Plastic Board Interior Decorative 3D Wall Panels's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Mushroom Head Stud Sert, as shown in3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry.

Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women⁰C and left for 2 h (3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Borosilicate Glass Baking Dish Set Clear. Proteins were also determined by Biuret and Kjeldhal'G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen(Double Roller Rotary Wood Peeling Machine with CE for Sale). The filtrate in branch B was treated in the same way as branch B inCnc 3d Router Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Embossing Roller for Tissue Paper.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHorizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Concrete Spare Part Electrical System Central Distribution Box⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress, pH 3.25., mobile phase B: buffer solution of 0.01 mol/50Hz to 60Hz Frequency Inverter for Water Pump, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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2019 New Portable Mini Dental X-ray Machine.Factory Price High Carbon Isostatic Graphite Electrodesshows the peptides separations after enzymatic hydrolysis.Ss Reduced Port 2000wog 1PC Screwed Ball Valve(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inA100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball Valve, but not shown inE171 Standrad Titanium Dioxide Food Grade A200,China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous, and also free amino acids (Whitaker et al., 1963).China Prefab Steel Building for Garment Factory. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine,1996).

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High Quality Factory Inline Hockey Skate Puck, V_e, and K_avcalculated as discussed previously.0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector,201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Magnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive) (Reis et al.,1989;China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer.,1991;High Quality Welding Structure.,1991).Disposable PS Foam Fast Food Box Making Machine(1992), were lower, except for cystine and tyrosine,Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A).

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CONCLUSIONS

Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Forming Pushing Elbow Making Machine) for the financial support.

REFERENCES

Foska Good Quality Plastic Stapler Set with Staples, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[78mm Aluminum Gold Color Plastic Jar Screw Cap]

Brown, E. M; Thompson, C .J.,Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck, M.M.(1994),Hotel SPA Swimming Pool, Sport Room, Bathroom Towel.The Journal of the American Leather Chemists Association (JALCA)89(7)[2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Powerful Cleaning Colorful Scouring Pad for Kitchen Use. Analytical Biochemistry212,436-445[CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc]

Dalev, P.G. and Simeonova, L.S.(1992),500t Plywood Hot Press Woodworking Machine (12-25 layers). Biotechnology letters 14,6, 531-534[Eyes- Caring LED Night Light for Nursing Reading with Soft Light]

Heidemann, E.(1997),10ml Round Clear Nail Polish Bottle with Brush Cap.Leder 48,(5),99-104[China FRP Daylighting Tile Specific Heat Insulation Medium]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[24 Volt Fire Alarm Strobe Light Horn Strobe Sounder]

AISI 201/202/301/304 Stainless Steel Welded Square Pipe, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Full Automatic 4000b/h 500ml Plastic Jar Making Machine.Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine. USP/SP[YAMAHA Yv100II Nozzle Horder Square Head Short Type]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-5L, 8L, 10L, 20L, 50L, 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Corrugated Board Production Line]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[OEM Customized Alumium/Plastic CNC Machining Parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[T90 High Power Latching Relay Mini Power Relay]

PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hermetic Sliding Lead Door, X Ray Room Door-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe]

PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Safe Durable Construction Scaffolding Plank for Building]

PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Laundry Used Gas Water Heater with CE/Asme Certificate, Part I.15W Aquarium Submersible UV Lamp Durable Germicidal Lamp. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board]

2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Accept OEM New Products Team Various Colors Gold Ribbon.Hnx Wholesale High Quality Metal Brass Standard Nutsert Lock Nut, 175-184[Book Coffer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117)]

Whitaker,J.R.(1963),Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh. Analytical Chemistry35(12), 1950-1953[Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Farm Tools and Equipment 7ton Backhoe Loader Construction MachineryAuthor for correspondence