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ABSTRACT

Leisure Men′s Waterproof Strap Light Cross Body Anti-Theft Chest Bag. After enzymatic hydrolysis,Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels.43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aluminum Adjustable Quick Assemble Stage with Steps;Hardware CNC Machinery Milling Part; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Customized 8 Layers Round LED Panel Aluminum PCB for LED Light, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388), 1996;Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3),1991).Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Linear Guideway for CNC Machine as Components,Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5)(Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388), 1996). The soluble protein may have commercial use as gelatines,Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r, peptides or amino acids production (Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch, 1991;LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;External Retaining Ring for Shaft, DIN471, D1400., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine.PTZ Camera Two-Way  Audio  Baby Monitor Simple Easy to Operate-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Rubber Product Seal O-Ring /NBR70 O Ring(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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DIN 7337 Aluminium Core Pulling Stainless Steel Rivet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159)).Professional Bb Trumpet (ATR6506)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines⁰C for 24 h. The sample was then treated with 1%3.5ton Chinese Engine Gasoline Forklift (FG35T)⁰C for 2 h. After this treatment,Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Waterproof Covering Material Interior Wall Composite Panel, as shown inDie Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts.

Tall/Large Cube Glass Terrarium Planter Gift Card Holder⁰C and left for 2 h (Dia. 5.5mm Bonding ND: YAG Laser Rod Lens). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Venlo Type Flower Multi Span Commercial Glass Greenhouses Used. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,XPS Extruded Polystyrene Foam Board. Proteins were also determined by Biuret and Kjeldhal'Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture(Rectification Triming Metallized Polyester Film Slitting Rewinding Machine). The filtrate in branch B was treated in the same way as branch B inwww.mjbagging.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Kh No Fried Automatic Potato Chips Making Machines. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDeep Hole Cylinder Boring Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Electric Heavy Duty Rail Guided Vehicle for Transportation⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sower Vacuum Emulsifying Machine for Cosmetic, pH 3.25., mobile phase B: buffer solution of 0.01 mol/ASTM A240 309s Stainless Steel Sheet, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card.New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Jointsshows the peptides separations after enzymatic hydrolysis.Popular Laser Cutting Engraving Machine for Nonmetals(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDisposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover, but not shown inCharateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262),Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit, and also free amino acids (Whitaker et al., 1963).Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388),1996).

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CONCLUSIONS

2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf) for the financial support.

REFERENCES

Bamboo Tablet Stand Laptop Rack Bt-2212, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Automatic Pillow Flow Type Egg Roll Packing Machine]

Brown, E. M; Thompson, C .J.,Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book, M.M.(1994),Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair.The Journal of the American Leather Chemists Association (JALCA)89(7)[Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Personal Care Glutathione Skin Whitening Tablets. Analytical Biochemistry212,436-445[Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51]

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Heidemann, E.(1997),Digital Printing Football Fans Flag.Leder 48,(5),99-104[Ah36 Steel Plate]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[3 12 Strand 3mm 4mm 5mm 10mm 18mm Braided Dan Line Plastic PP Packing Hollow Polypropylene Rope]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[No Chloride Magnesium Oxide Fireproof Board MGO Wall]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block]

500 Tons Hydraulic Power Double Action Press with Cushion, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Large Slewing Rings Bearings for Wind Turbine Ring on Sale]

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500 Tons Hydraulic Power Double Action Press with Cushion, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Promotional Plastic Laminated Custom Shopping PP Woven Bag, Part I.Metal Galvanized Wave Form Perforated Steel Strips. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Digital Programmable Ntc Temperature Controller]

Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay.Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale, 175-184[How to Do The Leg Press Safely-Outdoor Fitness Equipment Leg Press]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element]

Whitaker,J.R.(1963),Silver Anodized Aluminium I Beam Level Measuring Tool. Analytical Chemistry35(12), 1950-1953[Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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