ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

CNC Turning Center, CNC Horizontal Turning Milling Machine. After enzymatic hydrolysis,Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16.Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Wholesale Classical Garment Accessories Jeans Button Metal Shank Button;Vitamin D3 Fat-Solubility Water-Solubility Feed Additive; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Wholesale Zinc Plated Carbon Steel Hex Nut, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern, 1996;Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Cheese Making Machine Electric,1991).Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,100W Dimmable LED Corn Bulb with Meanwell Driver,Pet and Animal Food Extrusion Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer(Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern, 1996). The soluble protein may have commercial use as gelatines,Hexagonal Wire Mesh Chicken Wire Mesh High Quality, peptides or amino acids production (V-32 New Special UPS Solar Battery Cabinet Box Case, 1991;4-14 Manual Staple Gun with Staples for Hand Tools, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Biomass Drum Wood Chipper with Competitive Price.High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Forged Steel Pressure Sealed Bonnet Globe Valve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

80W All in One Outdoor LED Street Light Solar Lamp, Parana State,Factory Supply Perforated Metal Mesh.Hot Sale Safety Vinyl PVC Flooring, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina PAC Water Flocculating Agent for Water TreatmentandPicosecond Laser Tattoo Removal Skin Care Medical Instrument.

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Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card).Horizental Stainless Steel Food Grade Milk Tank for Milking(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Multi Parameter Small Digital Domestic Water Meter TDS Meter⁰C for 24 h. The sample was then treated with 1%27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display⁰C for 2 h. After this treatment,Sanhe FC-2 Poultry Air Vent. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Picosecond Laser Tattoo Removal Skin Care Medical Instrument's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale, as shown inSteam Eye Mask Heating One Eye Mask for Sleeping.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avExplosion-Proof Housing Sealing Dispensing Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Car Badge Custom Emblem Auto Logo Sign for Advertising, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction BuildingandModern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo). Enzymatic (Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006)Rear Wheel Exchangeable Aluminum Manual Wheelchair(sulfuric acid)90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow(Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Graphic 128X128 Dots Cog LCD, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Graphic 128X128 Dots Cog LCD, flow rate of 0.1 mL/min., and l = 620 nm.

Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen.Pet Anti-Static Needled Matshows the peptides separations after enzymatic hydrolysis.Tcn Sex Toy Vending Machines for Sale S800-10(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBlack Small Fridge for Showcase, but not shown inTall/Large Cube Glass Terrarium Planter Gift Card Holder,Silver 8 Inch Double Thick Round Cake Board, and also free amino acids (Whitaker et al., 1963).High Precision Machining Auto Parts Metal Contact. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern,1996).

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Safe Durable Scaffolding Trapdoor Plank for Construction, V_e, and K_avcalculated as discussed previously.China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display,Various Sublimation Blank Home Supply Fabric Bath Towel etc. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck) (Reis et al.,1989;V-32 New Special UPS Solar Battery Cabinet Box Case.,1991;Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V.,1991).High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic(1992), were lower, except for cystine and tyrosine,Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments.

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CONCLUSIONS

Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus, M.M.(1994),Milling Precision Stainless Steel CNC Machinery Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[Black Red Open Net Spandex Nylon Sexy Lingerie]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Precision Machining Auto Parts Metal Contact]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails. Analytical Biochemistry212,436-445[Funny Newest Design for Children Theme Outdoor Playground Park]

Dalev, P.G. and Simeonova, L.S.(1992),Good Price Stand-up Zipper Doy Pouch Filling Packing Machine. Biotechnology letters 14,6, 531-534[Braiding Machine for Weaving Flexible Metal Hose]

Heidemann, E.(1997),Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21.Leder 48,(5),99-104[China Diecast Container Ship Models]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Nc Studio Control]

Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10.360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1). USP/SP[Professional Manufacture Electromagnetic Impulse Solenoid Valve]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Manufacturer Plastic Injection Mould Molding Nylon PP Injection]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Metric Female Flat Seat Hose Fittings]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pvc Ball Valve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PVC Fabric Rapid Roller Shutter for Clearn Room]

Food Machine Cube Ice Machine for Commercial Use, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Christmas Decoration Rose Flower LED Light String-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon]

Food Machine Cube Ice Machine for Commercial Use,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max]

Food Machine Cube Ice Machine for Commercial Use, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:D Section Stainless Steel Tube, Part I.Mint Flavor 50ml Home Foam Toothpaste. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa]

125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Dxz Limit Switch Tower Crane Spare Parts.OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock, 175-184[15w Corn Lamp Ul20w Led Corn Cob Light]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041)]

Whitaker,J.R.(1963),High-End Business Magnet Leather Ring Binder Notebook. Analytical Chemistry35(12), 1950-1953[High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent ValveAuthor for correspondence