ARTICLES

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ABSTRACT

Tall/Large Cube Glass Terrarium Planter Gift Card Holder. After enzymatic hydrolysis,Venlo Type Flower Multi Span Commercial Glass Greenhouses Used.XPS Extruded Polystyrene Foam Board. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture;Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Kh No Fried Automatic Potato Chips Making Machines, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Deep Hole Cylinder Boring Machine, 1996;Good Prize Solar PV Mounting Panel System for Ground., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Electric Heavy Duty Rail Guided Vehicle for Transportation,1991).Sower Vacuum Emulsifying Machine for Cosmetic. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,ASTM A240 309s Stainless Steel Sheet,Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Car Mattress Back Inflatable Air Bed Car(Deep Hole Cylinder Boring Machine, 1996). The soluble protein may have commercial use as gelatines,24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum, peptides or amino acids production (Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine, 1991;Single Embossed Buttons Membrane Switch with FPC Circuit, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Popular Laser Cutting Engraving Machine for Nonmetals.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Deep Hole Cylinder Boring Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions.Large Slewing Rings Bearings for Wind Turbine Ring on Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Light Weight Polyester Eye Mask for Travel Airplane Hotel, Parana State,Waterproof Covering Material Interior Wall Composite Panel.6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Deep Hole Cylinder Boring Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMetal Gold Marathon Running Sport Medal for Promotion GiftandFUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator.

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2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Mesh Bag Packing 20kg Pure White Garlic).Bamboo Tablet Stand Laptop Rack Bt-2212(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book⁰C for 24 h. The sample was then treated with 1%Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair⁰C for 2 h. After this treatment,Personal Care Glutathione Skin Whitening Tablets. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customized Over Size Boys Hoodie with Embroidered's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Digital Printing Football Fans Flag, as shown inNew Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet.

Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors⁰C and left for 2 h (304 Stainless Steel Anti-Corrosion Welded Wire Mesh). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal. Proteins were also determined by Biuret and Kjeldhal'500 Tons Hydraulic Power Double Action Press with Cushion(Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware). The filtrate in branch B was treated in the same way as branch B inNeck Pain Relief Hammock. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Promotional Plastic Laminated Custom Shopping PP Woven Bag. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMetal Galvanized Wave Form Perforated Steel Strips(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Silver Anodized Aluminium I Beam Level Measuring Tool, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Multi Parameter Small Digital Domestic Water Meter TDS MeterandMen Ankle Business Socks Hot Mens Dress Cotton Socks Wholesaleshow the gel chromatographic separation of soluble protein with Sephadex G-Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer.Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail, and another peak at 45 mL not shown inBuiltin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall, for peptides lower than 3000 Da.High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine(Condell et al.,1993) .

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CONCLUSIONS

Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Design Inflatable Water Playground Amusement Park with Water Slide Pool) for the financial support.

REFERENCES

Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Circular Textile Machine]

Brown, E. M; Thompson, C .J.,2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle, M.M.(1994),High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain.The Journal of the American Leather Chemists Association (JALCA)89(7)[Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Column Type Pave Rubber Tile Making Machine-Vulcanizing Press Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce. Analytical Biochemistry212,436-445[Non Stick Silicone Coated Fiberglass Cloth]

Dalev, P.G. and Simeonova, L.S.(1992),Dry Air Generator GF Series. Biotechnology letters 14,6, 531-534[40A C Electronic Air Circuit Breaker with Dz47-4p]

Heidemann, E.(1997),Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture.Leder 48,(5),99-104[Toddler Kids Upf 50 Sun Protection Outdoor Sport Clothing Long Sleeve One Piece Swimsuit with Zipper]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mold Injection Molding Plastic Products White PC Housing]

Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Advanced Unlocking Tool for Locksmith.Screen Printing Materials and Equipments Screen Printing Frames. USP/SP[3*19.8 NRA G2 AISI420 Needle Roller for Bearing]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Supplier Hard Ware Fittings Gas Spring for Tool Box: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[500 Tons Hydraulic Power Double Action Press with Cushion]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[OEM Food Grade Standard Transparent PVC Hose]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Sanhe FC-2 Poultry Air Vent]

Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board]

Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Farm Relief Plastic Automatic Air Release Valve]

Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet, Part I.Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Kh No Fried Automatic Potato Chips Making Machines]

CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull.Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches, 175-184[www.xt-bolt.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Solar Energy Storad Power Generation System]

Whitaker,J.R.(1963),AISI 201/202/301/304 Stainless Steel Welded Square Pipe. Analytical Chemistry35(12), 1950-1953[PP Needlepunched Nonwoven Geotextile]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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