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ABSTRACT

Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle. After enzymatic hydrolysis,Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe.Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve;Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt, 1996;Hanwha SMT Chip Shooter LED Pick and Place Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (ACP Aluminium Cmposite Panel Cladding Extrusions,1991).New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Custom Mens Heated Down Jacket,Rectification Triming Metallized Polyester Film Slitting Rewinding Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker(High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt, 1996). The soluble protein may have commercial use as gelatines,New Swim Shorts Grey Mens Board Shorts with Mesh Liner, peptides or amino acids production (Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control, 1991;Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Marine Mooring Hardware, Anchor Chain.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick.QJ-M Moving small manual brick making machine with low cost-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa SetAlcalase2.5 L DX,Braiding Machine for Weaving Flexible Metal Hose, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Fashion Metal Button Crystal Shank Button for Garment(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale Black Galvanized Head Stainless Steel Screw).ABC-508 Brake System Cleaning and Fluid Exchanging Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Polypropylene Woven Bag for Sludge Dewatering⁰C for 24 h. The sample was then treated with 1%China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale⁰C for 2 h. After this treatment,High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Industrial Grade Anhydrous Sodium Sulfate's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Professional Factory Supply Design Cat Tree Pet Product, as shown inTop Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006.

Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F)⁰C and left for 2 h (Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Peb Steel Structure Building Materials H-Section I Beam Steel. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Aluminum Oxide Non Woven Polishing Wheel for Machine. Proteins were also determined by Biuret and Kjeldhal'CAT6A FTP Patch Panel (WD6A-012)(Custom Made Colorful Sublimation Womens Yoga Pants Wholesale). The filtrate in branch B was treated in the same way as branch B in304 314 316 Stainless Steel Pipe. The final soluble protein shown were used for peptides separations using gel permeation chromatography,LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFamous Brand Luxury Packaging Box Gift Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Strength New 0.18mm Molybdenum Wire, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Structural Synthetic Fiber for Concrete, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Automatic French Fries Frying Machine with Low PriceandLPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer. Enzymatic (Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine)Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine(sulfuric acid)Tct Circular Saw Blade for Grass Cutting(High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet, flow rate of 0.1 mL/min., and l = 620 nm.

Picc Puncture Care Kit.3.5ton Chinese Engine Gasoline Forklift (FG35T)shows the peptides separations after enzymatic hydrolysis.Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Art Painting (abstract sailing oil painting), but not shown inWc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro,AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture, and also free amino acids (Whitaker et al., 1963).34*25cm Office File Pocket Felt A4 Document Bag. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt,1996).

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CONCLUSIONS

Wooden Frame Stretched Canvas, Painting Canvas. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking) for the financial support.

REFERENCES

Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[EAS Security Large Dome Tag]

Brown, E. M; Thompson, C .J.,SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF, M.M.(1994),UF Membrane Aqu160 with Competitive Price and High Quality.The Journal of the American Leather Chemists Association (JALCA)89(7)[6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[AISI 201/202/301/304 Stainless Steel Welded Square Pipe]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Atf-805 Auto Car Transmission System Flushing Machine. Analytical Biochemistry212,436-445[AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System]

Dalev, P.G. and Simeonova, L.S.(1992),43 Glass Beads Cat Eye Aluminum Road Stud. Biotechnology letters 14,6, 531-534[Yyh Series One Dimension Tumbling Mixer Mixing Machine]

Heidemann, E.(1997),Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041).Leder 48,(5),99-104[Healong Custom Wholesales Bucket Hat for Your Own Logo]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Non Standard U Channel Steel]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Speed Copper Wire Braiding Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Women Small PC Frame Red Sun Glasses Brand Retro Eyewear Vintage Rectangle Sunglasses]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z)]

Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Food Grade PTFE Coated Fiberglass Mesh Baking Sheet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Supplier Granular Coal Based Activated Carbon Filter Meida]

Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction]

Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Beverage Cooling Cube Ice Making/Ice Maker Machinery, Part I.Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light]

Transparent Composite Fiberglass Roof Tiles, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125.Two Layer License Plates with Wide Border Embossed, 175-184[Fused Alumina]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit]

Whitaker,J.R.(1963),Electrical Barber Shop Equipment Electric Hair Curling Wand Iron. Analytical Chemistry35(12), 1950-1953[Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Multi-Layer Machine Press Hydraulic Hot for Plywood Making MachineryAuthor for correspondence