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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Single Stage High Speed Fruit and Vegetable Pulping Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board

ABSTRACT

Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services. After enzymatic hydrolysis,Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads.Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Prefab Expandable Luxury Living Container House Prefabricated House;Movable Partition Walls for Room Division, Hotel and Shopping Mall; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Carboxy Terminal Collagen Crosslinks Elisa Kits, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Various Colors Nylon and Stainless Steel Kitchen Tools, 1996;355nm UV Laser Marking Equipment for Crystal Materials., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Metal Separating Gold Brass Zippers for Sale,1991).Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,OEM Refrigeration Digital Thermometer,BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making(Various Colors Nylon and Stainless Steel Kitchen Tools, 1996). The soluble protein may have commercial use as gelatines,79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar, peptides or amino acids production (Two Roll Rubber & Plastic Mixing Mills Tire Machine, 1991;5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale 25kw Grid Tied Home Solar Panel System Kit., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Mini Car Portable Fridge 10L Mini Fridge.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Various Colors Nylon and Stainless Steel Kitchen Tools, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Mini Small Garden Excavator Digging Work.Professional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Offshore Drilling Platform Air Winch for Marine Oilfields(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label).Metal Gold Marathon Running Sport Medal for Promotion Gift(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery⁰C for 24 h. The sample was then treated with 1%Security Shopping Trolley Baby Seat for Supermarket Cart⁰C for 2 h. After this treatment,Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel, as shown inDigital Printing Football Fans Flag.

Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine⁰C and left for 2 h (High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Manual Foot Pedal Hydraulic Pump. Proteins were also determined by Biuret and Kjeldhal'860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag(60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light). The filtrate in branch B was treated in the same way as branch B in120 Mm Single Screw Extruder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Sale High Quality Rimless New Italy Design Titanium Eyeglasses for Business Men and Women.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CH Series Pid Intelligent Temperature Controller CH402. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Low Price Sweep Frequency Response Analyzer for Power Transformer Test⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Mechanic Parts for Lock Accessories with MIM Process, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Mesh Bag Packing 20kg Pure White Garlic. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Manual Interlocking Brick Making Machine) for the financial support.

REFERENCES

Supreme 524 Mv Sea Kayak, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.namarubber.com]

Brown, E. M; Thompson, C .J.,Trunk Organizer Foldable Water Proof, M.M.(1994),100%Polyester Burnout Velvet Sofa Fabric (pineapple grid).The Journal of the American Leather Chemists Association (JALCA)89(7)[Pre-Seasoned Cast Iron Mini Oval Casserole Pot]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Micro Small Plastic Product Injection Molding Making Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Diesel Generator Parts AVR Ki-Davr-95s for Kipor. Analytical Biochemistry212,436-445[Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Gld Brand Padlock Brass Popular in 5 Continents  . Biotechnology letters 14,6, 531-534[Custom Striped Compression Soccer Socks for Adults]

Heidemann, E.(1997),No Lead Bronze Flange Manufacturer.Leder 48,(5),99-104[Hot Sale Customized Universal Strachable Beer Bottle Silicone Bottle Cap]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Low Price Sweep Frequency Response Analyzer for Power Transformer Test]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[10 Sale! Rectangle Glass Dropper Bottle 30ml Amber Flat Glass Bottles for Vapor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap]

Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Laptop Bag College Men Backpack Leather Back Pack Bags-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[AISI 201/202/301/304 Stainless Steel Welded Square Pipe]

Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System]

Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pet Dog Grooming Rake Dematting Tool Shedding Comb, Part I.Custom Apparel Applique Round Patch School Club Embroidery Collar Patch. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Yyh Series One Dimension Tumbling Mixer Mixing Machine]

Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058.Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror, 175-184[Infrared Button Time Delay Manufacturers Cheap Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger]

Whitaker,J.R.(1963),Automatic Food Metal Detector Machine for Production Line. Analytical Chemistry35(12), 1950-1953[Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Non Standard U Channel SteelAuthor for correspondence