ARTICLES

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ABSTRACT

7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side. After enzymatic hydrolysis,High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes.Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8);Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Single Screw Extruder for Plastic PP/PE Pipe Production Line, 1996;Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage,1991).Gold Star Square Glass Candle Holder for Wedding Decoration. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,11kg 24L Low Pressure LPG Gas Cylinder,Commercial Use Click Lock Standard Spc Vinyl Plank Flooring,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop(Single Screw Extruder for Plastic PP/PE Pipe Production Line, 1996). The soluble protein may have commercial use as gelatines,High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng, peptides or amino acids production (Precision Customized OEM Auto Hardware Lathe CNC Machining Parts, 1991;White Color Enamel Bowls with High Quality, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Single Screw Extruder for Plastic PP/PE Pipe Production Line, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration.Southeast Asia Healthy Soft Integrated Latex Foam Mattress-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan, Parana State,Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine.Disposable Anesthesia Breathing Circuit Tube, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Single Screw Extruder for Plastic PP/PE Pipe Production Line, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh-Tenacity Conveyor Belt/ Steel Cord Conveyor BeltandChristmas Decoration Rose Flower LED Light String.

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High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen).Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126⁰C for 24 h. The sample was then treated with 1%Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks⁰C for 2 h. After this treatment,Counter-Clip Fluorine-Lined Butterfly Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)DC Brushless Motor for CNC Machine with ISO9001's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck, as shown inPurple UL 1095 16AWG Electronic Lead Wire.

Road Safety Warning Barricade LED Solar Powered Blinking Light⁰C and left for 2 h (Powerful Cleaning Colorful Scouring Pad for Kitchen Use). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Manufacturer Customized Plastic Injection Molding. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08). Proteins were also determined by Biuret and Kjeldhal'Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve(Popular Laser Cutting Engraving Machine for Nonmetals). The filtrate in branch B was treated in the same way as branch B inAlcohol Swap Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSolar Energy Storad Power Generation System(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Aluminum Cover Frame Half Aluminum Cover Profile⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility, pH 3.25., mobile phase B: buffer solution of 0.01 mol/DC24V 36W Solar Fan Motor PCBA Card, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Dry Air Generator GF SeriesandNew Monocular Hunting Wargame Mini Scope Digital Night Vision. Enzymatic (Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine)Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor(sulfuric acid)Italy Design Good Quality Hydraulic Motorcycle Lift Table(Single Screw Extruder for Plastic PP/PE Pipe Production Line, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Wholesale Menthol E Liquid E Juice for EGO E Cigarettes, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Wholesale Menthol E Liquid E Juice for EGO E Cigarettes, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

5in1 USB Type C Hub HDMI 4K USB C Hub to Ethernet RJ45 LAN Adapter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cabinet Shelf Support Pin Furniture Hardware Fittings) for the financial support.

REFERENCES

Hot Sale Coral Fleece Hotel Bathrobe (DPFT8070), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Lumen Searchlight]

Brown, E. M; Thompson, C .J.,Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone, M.M.(1994),Ladle Refining Melting Steel Furnace Casting Ladle.The Journal of the American Leather Chemists Association (JALCA)89(7)[460ml Glassware/Wine /Alcohol/Spirits/Glass Vodka Bottle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Lishui Factory High Quality Metal Rolling Ball Screw. Analytical Biochemistry212,436-445[Custom-Made Cotton Satin Towels for Adults with Thick Bath Towel]

Dalev, P.G. and Simeonova, L.S.(1992),Y-Spring Threaded Check Valve, 800 Wog. Biotechnology letters 14,6, 531-534[Coal Mine Mini Electric Excavator with Quick Coupling]

Heidemann, E.(1997),OEM CNC Processing Precision Plating/Anodizing/Painting Black Machining Part.Leder 48,(5),99-104[Hot Sale Injection Plastic 24 Bottle Beer Crate Mould]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress]

Power Fitting- Hot DIP Galvanizing Bolt with Nut, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Factory Custom Highly White Clear 750ml Tequila Liquor Glass Bottle.Galvanized Eye Grab Bend Hook. USP/SP[Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Transparent LED Display P3.91 Glass Wall LED Screen: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Price of a Retail Supermarket Push Cart for Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Full Automatic Carbonated Water Plastic Bottle Hot Shrinking Wrapping Packaging Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Enamel Cast Iron Mini Casserole Cast Iron Cocotte]

Surgical Power Tool Medical Care Orthopedic Mini Bone Saw, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Micro Small Plastic Product Injection Molding Making Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts]

Surgical Power Tool Medical Care Orthopedic Mini Bone Saw,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel]

Surgical Power Tool Medical Care Orthopedic Mini Bone Saw, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Glass Lifting Machine with Sucker for Glass Movements, Part I.Anodized Gold Brush Finish Aluminum Sheet Coil. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Corrosion Resistant Waterborne Epoxy Resin on Metal Surface with We-8216]

Popular Industrial Sewing Quilting Machine With CO, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace.Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate, 175-184[New Patented Wireless Recharge Teeth Whitening LED Light&Gel Pens Home Kit]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine]

Whitaker,J.R.(1963),Pure Sine Wave Power Frequency Inverter. Analytical Chemistry35(12), 1950-1953[High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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