ARTICLES

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ycycauto.comEdivaldo E.Garcia^I; Cláudia C.M.Carboxymethyl Cellulose CAS No. 9004-32-4^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip

ABSTRACT

150kg Per Hour Frozen French Fries Production Line. After enzymatic hydrolysis,Automatic Professional LED Display Ceramic Hair Curling Iron.CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer;Vulcanizing Machine /Rubber Tiles Vulcanzing Press; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce, 1996;Omni Newest Manager Desk Office Smart Office Table Office Staff Table., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap,1991).30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage,Full-Auto CNC Cutting Machine for Architecture Glass Sheet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product(Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce, 1996). The soluble protein may have commercial use as gelatines,Hot Forming Pushing Elbow Making Machine, peptides or amino acids production (13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike, 1991;Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;As568 Standard Brown FKM Rubber O Ring for Sealing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰200W UFO LED High Bay Light Fixture for Interior Exterior Applications.China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Embossing Roller for Tissue Paper(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A)).Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates⁰C for 24 h. The sample was then treated with 1%Colorful  Reflective Sticker Reflective Tape⁰C for 2 h. After this treatment,Concrete Spare Part Electrical System Central Distribution Box. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)50Hz to 60Hz Frequency Inverter for Water Pump, as shown inFolding Round Picnic Caravan Table RV Picnic Table Camping Table.

Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330)⁰C and left for 2 h (Walking Long Distance Surveying Instrument Meter Measuring Wheel). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels. Proteins were also determined by Biuret and Kjeldhal'Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator(Micro DC 24V Worm Gear Brushless Motor for Medical Equipment). The filtrate in branch B was treated in the same way as branch B inU Track/Channels Partition Wall /U Channels. The final soluble protein shown were used for peptides separations using gel permeation chromatography,PVC Cabinet Construction Board, White 18mm PVC Foam Board.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2019 New Portable Mini Dental X-ray Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSs Reduced Port 2000wog 1PC Screwed Ball Valve(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Prefab Steel Building for Garment Factory⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Dark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cable Tray with ′t′ Slots, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Carboxymethyl Cellulose CAS No. 9004-32-4and10 Gauge Galvanized Welded Wire Mesh for Construction Using. Enzymatic (High Quality Factory Inline Hockey Skate Puck)0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector(sulfuric acid)201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet(Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Disposable PS Foam Fast Food Box Making Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Disposable PS Foam Fast Food Box Making Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A).530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottleshows the peptides separations after enzymatic hydrolysis.Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBuy Mk 677 Powder Bulk Powders Sarms Mk 677, but not shown in40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5),Foska Good Quality Plastic Stapler Set with Staples, and also free amino acids (Whitaker et al., 1963).Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce,1996).

Eyeglass

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10ml Round Clear Nail Polish Bottle with Brush Cap, V_e, and K_avcalculated as discussed previously.Full Automatic 4000b/h 500ml Plastic Jar Making Machine,Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Made in China Lqa Strap Banding Tool) (Reis et al.,1989;13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike.,1991;Omni Newest Manager Desk Office Smart Office Table Office Staff Table.,1991).5L, 8L, 10L, 20L, 50L, 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine(1992), were lower, except for cystine and tyrosine,PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector.

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CONCLUSIONS

Hermetic Sliding Lead Door, X Ray Room Door. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women) for the financial support.

REFERENCES

Laundry Used Gas Water Heater with CE/Asme Certificate, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[AC drive]

Brown, E. M; Thompson, C .J.,15W Aquarium Submersible UV Lamp Durable Germicidal Lamp, M.M.(1994),2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b.The Journal of the American Leather Chemists Association (JALCA)89(7)[Folding Play Mat Crawl Play Mat]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Dbry-320 Christmas Stickers Labels Printing Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Accept OEM New Products Team Various Colors Gold Ribbon. Analytical Biochemistry212,436-445[High Density Celuca Kitchen Cabinets PVC Foam Board]

Dalev, P.G. and Simeonova, L.S.(1992),Hnx Wholesale High Quality Metal Brass Standard Nutsert Lock Nut. Biotechnology letters 14,6, 531-534[Fiber Optic Sc APC Fast Connector for FTTH Drop Cable]

Heidemann, E.(1997),Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh.Leder 48,(5),99-104[Compatible with Novametrix 505 510 511 515B/C 520 7000 7100 Oxypleth Adult finger clip SpO2 sensor Hyperstronic 6pin L=1m]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Self Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key]

Carboxymethyl Cellulose CAS No. 9004-32-4, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light.Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair. USP/SP[Aluminum Foil PE Bottle Cap Induction Seal Liner]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Racing Preformance PTFE Teflon Alloy Fuel Adapter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-What′s Kind of Machine for Making Organic Fertilizer Granules: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[110V DC Motor for Massager]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Robot of World Robotics Competition]

Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Home and Hospital Electric Patient Lift-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2mm Mirror Glass/Coated Glass for LED]

Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Food Machine Cube Ice Machine for Commercial Use]

Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles, Part I.M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Powder and Wax Brush with Wooden Handle]

Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),DC 12V Power Relay for Smart House.2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment, 175-184[Professional Cheap Bedding Quilt Polyester&Cotton Quilt for Airline]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable]

Whitaker,J.R.(1963),Medical Human Body Thermometer. Analytical Chemistry35(12), 1950-1953[FRP Water Tank GRP Fishing Tanks From Factory Supply]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Solar Panel Solar Cell Photovoltaic 300W Hot SellAuthor for correspondence