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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Titanium Dioxide Anatase for Paper Making. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,40A C Electronic Air Circuit Breaker with Dz47-4p

ABSTRACT

Pipe Thermostat with Sensor Ce Certificate. After enzymatic hydrolysis,Walking Long Distance Surveying Instrument Meter Measuring Wheel.Chinese Supplier of PVC Profile. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Tianjin Manufacturer PTFE Coated Butterfly Valve;Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Prices of 20W 40W 60W Solar Street Lights, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sinicline Custom Brand with Plastic Pouch Hangtag for Hats, 1996;Legit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag,1991).VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,ALS Stage 5W RGB Animation Laser Light,Multifunctional Solar Powered Safety Hammer LED Flashlight, Alarm Fescape Rescue LED Torch,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light(Sinicline Custom Brand with Plastic Pouch Hangtag for Hats, 1996). The soluble protein may have commercial use as gelatines,Pet Anti-Static Needled Mat, peptides or amino acids production (Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine, 1991;OEM Sheet Metal Fabrication for Aluminum Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hwf Tsp Core Bit for Geotechnical Drilling., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Price of Automatic Sludge Dewatering Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sinicline Custom Brand with Plastic Pouch Hangtag for Hats, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Worm Gear Operation Wafer Lined F3 Butterfly Valve.Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Original Testo 174t Mini Temperature Data Logger No. 0572 1560, Parana State,40A C Electronic Air Circuit Breaker with Dz47-4p.Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Sinicline Custom Brand with Plastic Pouch Hangtag for Hats, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCorrugated Board Production LineandT90 High Power Latching Relay Mini Power Relay.

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Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Safe Durable Construction Scaffolding Plank for Building).New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Whole Set Gas Spring Strut for Furniture⁰C for 24 h. The sample was then treated with 1%Dia. 5.5mm Bonding ND: YAG Laser Rod Lens⁰C for 2 h. After this treatment,CNC Machining Turning Anodized Aluminum Worm Gear. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Solar Panel Solar Cell Photovoltaic 300W Hot Sell's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Vacuum Coating Machine Water Chiller (CW-6300AN), as shown inFactory Manufacturers Prices Melamine Faced Chipboard/Particle Board.

Planter Moving Round Planter Saucer Casters Plant Trolley (M)⁰C and left for 2 h (Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X). Proteins were also determined by Biuret and Kjeldhal'Custom ABS&POM&PP&PC&Acrylic Plastic CNC Machining Turning Parts(Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup). The filtrate in branch B was treated in the same way as branch B inTork Twist Lock Photocell Supplier & Manufacturer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,CNC Machining Non-Standard Hardware Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%DOT Aluminum Beverage CO2 Gas Cylinder 5lb 10lb 15lb 20lb. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGalvanized Solar System Mounting Bracket Connector(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Water Meter Spare Parts Rubber Rings for Sealing⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainless Steel HK Series Needle Roller Bearing HK0408, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Road Construction Fabric Non-Woven Geotextile 200GSM, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Farm Tools and Equipment 7ton Backhoe Loader Construction MachineryandHigh Polymer Modified Cement Based Elastic Waterproof Coating. Enzymatic (Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe)Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset(sulfuric acid)Fashion Shoulder Backpack Handbag School Bag(Sinicline Custom Brand with Plastic Pouch Hangtag for Hats, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Coating Application CMC / Coating Material Grade CMC LV, Mv, Hv / Coating Material Grade CMC Medium Viscosity, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Coating Application CMC / Coating Material Grade CMC LV, Mv, Hv / Coating Material Grade CMC Medium Viscosity, flow rate of 0.1 mL/min., and l = 620 nm.

Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl.Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plantshows the peptides separations after enzymatic hydrolysis.FTTH Fiber Optic Fusion Splicing Toolkit(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull, but not shown inADC12 Aluminum Die Casting Parts,High Speed Cable Wire Winding Braiding Machine, and also free amino acids (Whitaker et al., 1963).22 High Rubber Mixing Mill, High Accuracy Lab Rubber Two Roll Mill. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Sinicline Custom Brand with Plastic Pouch Hangtag for Hats,1996).

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CONCLUSIONS

Maxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (S Series China Generator Helical-Worm Crushers Reduction Box) for the financial support.

REFERENCES

3HP Industrial Cooling System Water Cooled Chiller, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[20*10 Inch Black Milling Truck Car Rims PCD 127-180 Concave off-Road Alloy Wheels]

Brown, E. M; Thompson, C .J.,OEM/ODM Custom Made Muslim Bridal Wedding Gown Dress, M.M.(1994),Wood Veneer Peeling and Clipping Combined Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[100W 120W 150W Outdoor Solar LED Street Lighting]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale Original Smartphone for Huawei P20 Lite Global Firmware 4G Lte Mobile Phone Face ID 5.84 Screen Android 8.0 Front Camera 4GB 128GB ROM Cellphone. Analytical Biochemistry212,436-445[PAC /Poly Aluminium Chloride for Water Treatment]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale High Quality Decoration Oil Painting, Home Decoration Painting, Art Painting (abstract sailing oil painting). Biotechnology letters 14,6, 531-534[Galvanized Roller Shutter Window Torsion Springs]

Heidemann, E.(1997),Si3n4 Full Ceramic  Bearing  608 Size 8X22X7.Leder 48,(5),99-104[High Capacity 200-1000t/H Primary Mobile Jaw Crusher Machinery Price]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Italy Design Good Quality Hydraulic Motorcycle Lift Table]

CNC System Air Duct Line5 Make Machine for HVAC, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall.Kara Two-Way Dual 8′′ Line Array PRO Audio System Powerful PA Speaker. USP/SP[6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Corrugated Board Production Line: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Blow Mold Deer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[White Ceramic Shot Glass for Sublimation Printing - 1.5oz]

Magnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Car Air Conditional Compressor Magetic Clutch 5pk-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Graphite Ring Good Lubrication]

Magnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CNC System Air Duct Line5 Make Machine for HVAC]

Magnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Engieered Veneer Faced Commericial Plywood, Part I.Hex Thin Head Nut. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Famous Brand Luxury Packaging Box Gift Box]

Pharmaceutical Peptide Ghrp 2 Acetate CAS158861-67-7 Ghrp-2, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Big Capacity Crystal Clear Glass Wine Bottle with Screw Cap.Spare Parts for Caterpillar Construction Machinery, 175-184[Summer Children Printed Pink Round Neck T Shirt Girls Plain Cotton Short Sleeve Clothes]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Full-Auto CNC Cutting Machine for Architecture Glass Sheet]

Whitaker,J.R.(1963),Hpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank. Analytical Chemistry35(12), 1950-1953[Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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