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ABSTRACT

Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430. After enzymatic hydrolysis,Polyglycolic Acid Absorable Suture.No. 1062 20PCS Tools. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant;Aluminum Mould Shoe Mould Making CNC Milling Router Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Pressure Fitting Quick Coupling Hydraulic Plug, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover, 1996;Refrigeration Digital Temperature Controller Stc-100A., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle,1991).Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Black Color Hexagon Bolt with High Quality,China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price(High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover, 1996). The soluble protein may have commercial use as gelatines,Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag, peptides or amino acids production (High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton, 1991;Back Adhesive 72W Solar Flexible PV Laminate Panels (PVL-72), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Non Rechargeable, NiMH Replacement 1.5V Lr44/V13ga Battery., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Single Stage High Speed Fruit and Vegetable Pulping Machine.China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Carboxy Terminal Collagen Crosslinks Elisa Kits, Parana State,Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch.Various Colors Nylon and Stainless Steel Kitchen Tools, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale Menthol E Liquid E Juice for EGO E CigarettesandFactory OEM CNC Machined Gear Motor Shaft.

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355nm UV Laser Marking Equipment for Crystal Materials(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( COB Stn LCD Display with 8080 Interface).Metal Separating Gold Brass Zippers for Sale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate⁰C for 24 h. The sample was then treated with 1%OEM Refrigeration Digital Thermometer⁰C for 2 h. After this treatment,BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar, as shown inFactory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag.

Two Roll Rubber & Plastic Mixing Mills Tire Machine⁰C and left for 2 h (Carboxy Terminal Collagen Crosslinks Elisa Kits). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Sale 25kw Grid Tied Home Solar Panel System Kit. Proteins were also determined by Biuret and Kjeldhal'Mini Car Portable Fridge 10L Mini Fridge(Popular Custom School Uniform Woven Badge Patch with Merrow Border). The filtrate in branch B was treated in the same way as branch B in32ton Hydraulic Bottle Jack. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mini Small Garden Excavator Digging Work. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avProfessional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Mass Stock Yl10.2 Carbide Rods for Milling 45HRC⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Heavy Industry Electric Transport Vehicle on Rails, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Manual Interlocking Brick Making Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery.Rear Wheel Exchangeable Aluminum Manual Wheelchairshows the peptides separations after enzymatic hydrolysis.Security Shopping Trolley Baby Seat for Supermarket Cart(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStainless Steel Body Infrared Burner Good Selling Gas Stove, but not shown inHigh - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags,Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24, and also free amino acids (Whitaker et al., 1963).Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover,1996).

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CONCLUSIONS

Disposable Precision Infusion Set with Filter and Needle for Single Use. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Prefab Expandable Luxury Living Container House Prefabricated House) for the financial support.

REFERENCES

Low Price Sweep Frequency Response Analyzer for Power Transformer Test, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Click Lock Waterproof Plastic Vinyl Plank Spc Flooring Tile]

Brown, E. M; Thompson, C .J.,Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500, M.M.(1994),Stainless Steel Mechanic Parts for Lock Accessories with MIM Process.The Journal of the American Leather Chemists Association (JALCA)89(7)[Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Foldable Esg10310]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT. Analytical Biochemistry212,436-445[Decorative House Stone Coated Metal Roof Tile]

Dalev, P.G. and Simeonova, L.S.(1992),Mining Machinery Sand Vibrating Sieve for River Sand. Biotechnology letters 14,6, 531-534[Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2+1 Flavors Soft Ice Cream Machine with Pre-Cooling]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cheap Price Paper Straws Individually Wrapped Drinking Straw]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Adapter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh]

High Quality MPO Singlemode Duplex Fiber Optic Adapter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hotel Bedding-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System]

High Quality MPO Singlemode Duplex Fiber Optic Adapter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale]

High Quality MPO Singlemode Duplex Fiber Optic Adapter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Supplier V Wedge Wire Johnson Water Well Screen, Part I.Clear Black Empty 15ml 30ml 50ml 100ml Frosted White Square Glass Perfume Bottles. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood]

10A MPPT Solar Controllers PV 60V for off-Grid, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears.Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole, 175-184[80cm Cleaning Mop for Wet and Dry Using Cotton Mop]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Pagoda Gazebo Tent in Guangzhou for Sale]

Whitaker,J.R.(1963),60ml 100ml Customized Glass Jar for Spice, Candle, Candy. Analytical Chemistry35(12), 1950-1953[Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Stainless Steel Mechanic Parts for Lock Accessories with MIM ProcessAuthor for correspondence