ARTICLES

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ABSTRACT

Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb. After enzymatic hydrolysis,Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting.Toilet Roll Tissue Wrapping Machine with Auto - Counting. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine;Orange Monofilament Fishing Tackle Nylon Fish Net; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (By6-8-200-L Automatic Doypack Premade Pouch Packing Machine, 1996;Diameter 1300mm Lifting Electro Magnet for Cast Iron., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%,1991).Evaporator Finned Tube. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear,Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,100%Polyester Spandex Hotel Chair Cover (DPH7022)(By6-8-200-L Automatic Doypack Premade Pouch Packing Machine, 1996). The soluble protein may have commercial use as gelatines,Factory Supply Perforated Metal Mesh, peptides or amino acids production (CNC Turning Center, CNC Horizontal Turning Milling Machine, 1991;Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Classical Garment Accessories Jeans Button Metal Shank Button.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Vitamin D3 Fat-Solubility Water-Solubility Feed Additive. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (By6-8-200-L Automatic Doypack Premade Pouch Packing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Wholesale Zinc Plated Carbon Steel Hex Nut.Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer, Parana State,48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System.Hexagonal Wire Mesh Chicken Wire Mesh High Quality, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (By6-8-200-L Automatic Doypack Premade Pouch Packing Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGood Price Stand-up Zipper Doy Pouch Filling Packing MachineandSolar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road.

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V-32 New Special UPS Solar Battery Cabinet Box Case(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts).4-14 Manual Staple Gun with Staples for Hand Tools(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan⁰C for 24 h. The sample was then treated with 1%Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine⁰C for 2 h. After this treatment,Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Biomass Drum Wood Chipper with Competitive Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags, as shown inRoll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh.

Forged Steel Pressure Sealed Bonnet Globe Valve⁰C and left for 2 h (ANSI C29 25kv 1pin Hole F Neck Modified Polyethylene HDPE Insulator). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Plastic Injection Moulding Machine Supplier / Factory /Manufacturer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels. Proteins were also determined by Biuret and Kjeldhal'15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable(Parcel Tape Adhesive OPP Packing Tape). The filtrate in branch B was treated in the same way as branch B in920 Stationary Bike With 24kgs Flywheel amp Spd Pedal. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%80W All in One Outdoor LED Street Light Solar Lamp. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sale Safety Vinyl PVC Flooring(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Horizental Stainless Steel Food Grade Milk Tank for Milking⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Multi Parameter Small Digital Domestic Water Meter TDS Meter, pH 3.25., mobile phase B: buffer solution of 0.01 mol/27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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SUS316 Hexagon Socket Set Screws with Cone Point, DIN914.Lightweight Adjustable Dorsal Ankle Night Splint for Pain Reliefshows the peptides separations after enzymatic hydrolysis.Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products, but not shown inChina Supplier Hard Ware Fittings Gas Spring for Tool Box,Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor, and also free amino acids (Whitaker et al., 1963).Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (By6-8-200-L Automatic Doypack Premade Pouch Packing Machine,1996).

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Car Badge Custom Emblem Auto Logo Sign for Advertising, V_e, and K_avcalculated as discussed previously.2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt,Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror) (Reis et al.,1989;CNC Turning Center, CNC Horizontal Turning Milling Machine.,1991;Diameter 1300mm Lifting Electro Magnet for Cast Iron.,1991).Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006(1992), were lower, except for cystine and tyrosine,Rear Wheel Exchangeable Aluminum Manual Wheelchair.

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CONCLUSIONS

90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (100W Dimmable LED Corn Bulb with Meanwell Driver) for the financial support.

REFERENCES

Graphic 128X128 Dots Cog LCD, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Kindergarten Rubber Tile]

Brown, E. M; Thompson, C .J.,Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen, M.M.(1994),Tcn Sex Toy Vending Machines for Sale S800-10.The Journal of the American Leather Chemists Association (JALCA)89(7)[90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Silver 8 Inch Double Thick Round Cake Board. Analytical Biochemistry212,436-445[36PCS LED Gold Matrix Stage Zoom Light]

Dalev, P.G. and Simeonova, L.S.(1992),High Precision Machining Auto Parts Metal Contact. Biotechnology letters 14,6, 531-534[Glow Powder for Luminous Coating]

Heidemann, E.(1997),Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label.Leder 48,(5),99-104[Industrial Waste Water Recycling Machine Manufacturer Price]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Welding Structure]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Safe Durable Scaffolding Trapdoor Plank for Construction: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fiberglass Animal Poultry Insulation Grain Tower]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Hebei Custom Made Alloy Steel Forging Gear Coupling]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Supreme 524 Mv Sea Kayak]

China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Various Sublimation Blank Home Supply Fabric Bath Towel etc-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory]

China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels]

China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic, Part I.Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit]

Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Best Quality Bending Metal Mould with All Kinds Metal Materials.New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus, 175-184[1-10 Colored Index Divider]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike]

Whitaker,J.R.(1963),Milling Precision Stainless Steel CNC Machinery Parts. Analytical Chemistry35(12), 1950-1953[8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round PipeAuthor for correspondence