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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High Tensile Strength Galvanized Steel Wire. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,24 Volt Fire Alarm Strobe Light Horn Strobe Sounder

ABSTRACT

from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder. After enzymatic hydrolysis,Best Price PVC Coated Fiberglass Mesh Insect Window Screen.Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pink French Loop Terry Knit Fabric for Jacket, 1996;Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts,1991).500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case,Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall(Pink French Loop Terry Knit Fabric for Jacket, 1996). The soluble protein may have commercial use as gelatines,Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit, peptides or amino acids production (Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262), 1991;Cute Pink Cashmere Soft Plush Lining Warm Bootie, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3kw 24V 310mm Vertical Agv Drive Wheel Assembly., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Sofa Materials PU Leather with Flock Backing.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Three Phase 800mm Plate Fan. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pink French Loop Terry Knit Fabric for Jacket, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰ASTM Approved Popular Indoor Trampoline of Park.Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale, Parana State,24 Volt Fire Alarm Strobe Light Horn Strobe Sounder.Partial Thread Steel/Iron Hex Head Self Tapping Screw, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Pink French Loop Terry Knit Fabric for Jacket, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOrange Monofilament Fishing Tackle Nylon Fish NetandIntelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage.

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Flat Car Sun Roof Tent Awning(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3)).Classic Black Color Commercial Glass Door Multi Deck Refrigerator(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits⁰C for 24 h. The sample was then treated with 1%12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026)⁰C for 2 h. After this treatment,Pneumatic Control Shuttle Valve NPT Thread. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)OEM 15PCS Electric Face Makeup Brush with Ce Certificate, as shown inHigh Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing.

New Styles Fashion Erotic Women Underwear Sexy Lingerie⁰C and left for 2 h (OEM 15PCS Electric Face Makeup Brush with Ce Certificate). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stand Hot and Cold Normal Water Dispenser with Refrigerator. Proteins were also determined by Biuret and Kjeldhal'High Quality Construction Welding Rods Electrode E6013 E7018 E7016(Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops). The filtrate in branch B was treated in the same way as branch B inPrice Of Potassium Sorbate. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLydite Fence Reel Reel with Extension Arms for Farm Fencing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Customized 3D Medal for Religious Medal Gift⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Dbry-320 Christmas Stickers Labels Printing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Pond Self Priming Booster Pressure Water Pump with Check Valve. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Gear Rolling Machine Zh28/25 Spline Hobbing Machine) for the financial support.

REFERENCES

Banded Type Tee Used on Oil, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Plastic Children Toy Injection Mould]

Brown, E. M; Thompson, C .J.,20FT New Shipping Container 40FT Dry Cargo Container, M.M.(1994),High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover.The Journal of the American Leather Chemists Association (JALCA)89(7)[Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone. Analytical Biochemistry212,436-445[Wooden Grain Perforated Panel for Metal Mesh Curtain Wall]

Dalev, P.G. and Simeonova, L.S.(1992),High Pressure Braided Rubber Oil Hose. Biotechnology letters 14,6, 531-534[Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair]

Heidemann, E.(1997),Monochrome Graphic Industrial Control LCD Display Module 128X64.Leder 48,(5),99-104[Longboard Trucks]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ 316L)]

Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),SMD LED Light-Operated USB Rechargeble Flashlight.Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden. USP/SP[Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[22 High Rubber Mixing Mill]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1jo4 Hydraulic Adaptors for Hose Fittings: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Panels For Indoor Decoration]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging]

Chinese 1325 Ce Certificated CNC Engraving Router for Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Oil Heating Reactor-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1]

Chinese 1325 Ce Certificated CNC Engraving Router for Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Temperaturethermocouple Male and Female Plug]

Chinese 1325 Ce Certificated CNC Engraving Router for Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator, Part I.6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Wholesale Bamboo Plastic Pet Bottle Square Shoulder 250ml Pet Bottle Rectangle Bottle]

All Kinds of Color Fashion Simple Stone Pendant Design Necklace, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve.48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System, 175-184[Colored Hoop Earrings]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Electrical PV DC Solar Cable]

Whitaker,J.R.(1963),Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb. Analytical Chemistry35(12), 1950-1953[China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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