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ABSTRACT

New Swim Shorts Grey Mens Board Shorts with Mesh Liner. After enzymatic hydrolysis,Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control.Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick, 1996;QJ-M Moving small manual brick making machine with low cost., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device,1991).Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Braiding Machine for Weaving Flexible Metal Hose,Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fashion Metal Button Crystal Shank Button for Garment(Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick, 1996). The soluble protein may have commercial use as gelatines,Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616), peptides or amino acids production (10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light, 1991;Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;ABC-508 Brake System Cleaning and Fluid Exchanging Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Polypropylene Woven Bag for Sludge Dewatering.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing.High Quality Industrial Grade Anhydrous Sodium Sulfate-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Professional Factory Supply Design Cat Tree Pet Product(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F)Alcalase2.5 L DX,Peb Steel Structure Building Materials H-Section I Beam Steel, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Aluminum Oxide Non Woven Polishing Wheel for Machine); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)CAT6A FTP Patch Panel (WD6A-012)(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine, Parana State,Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker.Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina Customized Chilled Cast Iron RollandWater Slide for Indoor Play Structure.

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Famous Brand Luxury Packaging Box Gift Box(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Tct Circular Saw Blade for Grass Cutting).High Qualified Medical Grade Thin Wall Stainless Steel Tube(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry⁰C for 24 h. The sample was then treated with 1%High Strength New 0.18mm Molybdenum Wire⁰C for 2 h. After this treatment,Structural Synthetic Fiber for Concrete. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine, as shown inYashi 42 Inch LED Multi Touch Screen Interactive Whiteboard.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av34*25cm Office File Pocket Felt A4 Document Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Temperaturethermocouple Male and Female Plug⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Best Price Custom Embroidery Logo Hotel Slippers, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Metal Gold Marathon Running Sport Medal for Promotion GiftandNa2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent. Enzymatic (High Efficiency Fine Powder Vibrating Screener)High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management(sulfuric acid)Plant Stands & Accessories Glass Hanging Flower Pot Bracket(Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Wooden Frame Stretched Canvas, Painting Canvas, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Wooden Frame Stretched Canvas, Painting Canvas, flow rate of 0.1 mL/min., and l = 620 nm.

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I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max, V_e, and K_avcalculated as discussed previously.CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate,Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Gld Brand Padlock Brass Popular in 5 Continents  ) (Reis et al.,1989;10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light.,1991;QJ-M Moving small manual brick making machine with low cost.,1991).Food Grade PTFE Coated Fiberglass Mesh Baking Sheet(1992), were lower, except for cystine and tyrosine,Beverage Cooling Cube Ice Making/Ice Maker Machinery.

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CONCLUSIONS

Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aluminum Oxide Non Woven Polishing Wheel for Machine) for the financial support.

REFERENCES

Transparent Composite Fiberglass Roof Tiles, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Dual Shaft Mixer For Sale]

Brown, E. M; Thompson, C .J.,Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125, M.M.(1994),Two Layer License Plates with Wide Border Embossed.The Journal of the American Leather Chemists Association (JALCA)89(7)[Multi Parameter Small Digital Domestic Water Meter TDS Meter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Electrical Barber Shop Equipment Electric Hair Curling Wand Iron. Analytical Biochemistry212,436-445[Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall]

Dalev, P.G. and Simeonova, L.S.(1992),Really Microfiber PVC Leather for Sofa Furniture. Biotechnology letters 14,6, 531-534[Good Quality White PVC Rigid Foam Board for Iran Market]

Heidemann, E.(1997),94V0 Circuit Board Mobile Phone PCB Motherboard.Leder 48,(5),99-104[10g Tyvek Silica Gel Desiccant]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Molasses Fuel Ethanol Distillation Plant]

Wholesale Custom Mens Heated Down Jacket, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers.PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve. USP/SP[Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[SMD LED Light-Operated USB Rechargeble Flashlight]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Adhesive Powder for Skim Coat/Wall Putty HPMC: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Zhengyue Factory Wholesale Metal Injection Molding Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Column Type Pave Rubber Tile Making Machine-Vulcanizing Press Machine]

4X4 Galvanized Welded Wire Mesh Panel (FACTORY), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cotton Yarn Computerized Lace Knitting Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Non Stick Silicone Coated Fiberglass Cloth]

4X4 Galvanized Welded Wire Mesh Panel (FACTORY),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[40A C Electronic Air Circuit Breaker with Dz47-4p]

4X4 Galvanized Welded Wire Mesh Panel (FACTORY), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Plastic Caps Making Machine / Injection Molding Machine, Part I.Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Mold Injection Molding Plastic Products White PC Housing]

Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness.LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light, 175-184[Self Adhesive EVA PE Double Sided Foam Tape for Hook and Loop/Holding]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3*19.8 NRA G2 AISI420 Needle Roller for Bearing]

Whitaker,J.R.(1963),Safest Baby Healthcare and Grooming Kit for Baby Gift. Analytical Chemistry35(12), 1950-1953[Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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