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ABSTRACT

Digital Printing Football Fans Flag. After enzymatic hydrolysis,Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors.Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Promotional Plastic Laminated Custom Shopping PP Woven Bag, 1996;Metal Galvanized Wave Form Perforated Steel Strips., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12),1991).Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale,Silver Anodized Aluminium I Beam Level Measuring Tool,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring(Promotional Plastic Laminated Custom Shopping PP Woven Bag, 1996). The soluble protein may have commercial use as gelatines,48V25A Lead Acid Battery Maintenance Pulse Charger, peptides or amino acids production (15mm Best Price Commercial Grade Birch Plywood for Furniture, 1991;Top Great Wallet Genuine Leather Purse with Coin Pocket, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Good Price 20kVA Single Phase Silent Diesel Generator., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Promotional Plastic Laminated Custom Shopping PP Woven Bag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls.Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil).Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002)⁰C for 24 h. The sample was then treated with 1%2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle⁰C for 2 h. After this treatment,High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Dry Air Generator GF Series, as shown inPipe Thermostat with Sensor Ce Certificate.

Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture⁰C and left for 2 h (Gold Star Square Glass Candle Holder for Wedding Decoration). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Advanced Unlocking Tool for Locksmith. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Screen Printing Materials and Equipments Screen Printing Frames. Proteins were also determined by Biuret and Kjeldhal'China Supplier Hard Ware Fittings Gas Spring for Tool Box(Solid Flex-Drain Pipe Length 40′). The filtrate in branch B was treated in the same way as branch B inLaser Hair Removal 808nm. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road.Laparoscopy Clip Applicator for Abdominal Surgery Usesshows the peptides separations after enzymatic hydrolysis.High Speed Copper Wire Braiding Making Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWaterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank, but not shown inLifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook,Safe Durable Construction Scaffolding Plank for Building, and also free amino acids (Whitaker et al., 1963).Different Size of Ring NdFeB Magnet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Promotional Plastic Laminated Custom Shopping PP Woven Bag,1996).

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Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story BookandPVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Capsshow the gel chromatographic separation of soluble protein with Sephadex G-Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine.High Quality Welding Structure, and another peak at 45 mL not shown inDxz Limit Switch Tower Crane Spare Parts, for peptides lower than 3000 Da.Carboxymethyl Cellulose CAS No. 9004-32-4(Condell et al.,1993) .

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CONCLUSIONS

China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stainless Steel Clarifier Tank for Water Treatment) for the financial support.

REFERENCES

Vulcanizing Machine /Rubber Tiles Vulcanzing Press, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Minimalism Small Portable Rotary Axis for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup]

Brown, E. M; Thompson, C .J.,Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low, M.M.(1994),Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Omni Newest Manager Desk Office Smart Office Table Office Staff Table. Analytical Biochemistry212,436-445[OEM 15PCS Electric Face Makeup Brush with Ce Certificate]

Dalev, P.G. and Simeonova, L.S.(1992),6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap. Biotechnology letters 14,6, 531-534[Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops]

Heidemann, E.(1997),30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps.Leder 48,(5),99-104[1800lbs Portable Hand Small Manual Brake Boat Winch with Ratchet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe]

Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage.Full-Auto CNC Cutting Machine for Architecture Glass Sheet. USP/SP[New Condition Hot Sale Type Air Cooled Ice Cube Maker]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Washami Teeth Care for Whitening Cool Mint Toothpaste]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Competitve Price Aluminium LED Profile Extrusion with High Quality]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Price of Automatic Sludge Dewatering Machine]

Hot Forming Pushing Elbow Making Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[D Section Stainless Steel Tube]

Hot Forming Pushing Elbow Making Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah]

Hot Forming Pushing Elbow Making Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner, Part I.As568 Standard Brown FKM Rubber O Ring for Sealing. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cotton Yarn Computerized Lace Knitting Machine]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger]

Whitaker,J.R.(1963),China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2). Analytical Chemistry35(12), 1950-1953[Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Workstation Glass Frame Extrusion Aluminum Profiles for Office PartitionsAuthor for correspondence