ARTICLES

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ABSTRACT

High Quality Stainless Steel Expanded Metal Mesh. After enzymatic hydrolysis,Popular Lovely Animal Design Inflatable Slide for Kids.Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable;Hairs Comb for Pet Dog Cats; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine, 1996;Australia C-Tick Testing and Certification Service for Electric Scooter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,,1991).35 Degree Escalator with Auto Start Function in Parallel. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings,Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Roller Wedge Screen Cylinder(Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine, 1996). The soluble protein may have commercial use as gelatines,Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup, peptides or amino acids production (Push Pull Small Cable to Cable Connector, 1991;Stainless Steel Seamless Pipe ASTM A312 TP304 316, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Big Chocolate Storage Holding Tank, Chocolate Buffer Tank. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce.Asco 353 Series 3/4 Pulse Valve Solenoid Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Fine Mist Plastic 0.34 Oz Spray 100ml Airless BottleAlcalase2.5 L DX,Purple UL 1095 16AWG Electronic Lead Wire, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Brushed Anodizing Aluminum Extrusion Profile for LED); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

S235jo S235jr Steel Pipe Tube Shs Rhs, Parana State,Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170.Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFlexible Indoor P4.81 500*500mm LED Display Screen for Video WallandHeat Pump Food Cabinet Dog Food Pet Food Dryer Machine.

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Pre-Seasoned Cast Iron Mini Oval Casserole Pot(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket).Stainless Steel Square Wire Mesh Netting with SGS(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter⁰C for 24 h. The sample was then treated with 1%Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display⁰C for 2 h. After this treatment,Outdoor Garden Save Energy with Solar Flame Wall Light. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag, as shown inG08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen.

Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length⁰C and left for 2 h (Customized Souvenirs Tin Metal Number & Name Plate for Car). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Non Stick Silicone Coated Fiberglass Cloth. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502. Proteins were also determined by Biuret and Kjeldhal'Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius(XPS Extruded Polystyrene Foam Board). The filtrate in branch B was treated in the same way as branch B in98% Price Copper Sulphate. The final soluble protein shown were used for peptides separations using gel permeation chromatography,ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Sale 100% Polyester Cartoon Kids Sleepwear⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Custom Rubber Product Seal O-Ring /NBR70 O RingandLaparoscopy Clip Applicator for Abdominal Surgery Uses. Enzymatic (High Yield Tree Stump Crusher Machine)Diaphragm Valve for Bag Filter Since 1992(sulfuric acid)4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf(Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Manufacturer Plastic Injection Mould Molding Nylon PP Injection.Modern 4 Person Workstation with Computer Desk Modular Office Furnitureshows the peptides separations after enzymatic hydrolysis.Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board, but not shown inLocksmith Tool Car Lock Transpopnder Chip Key Blank Cover,High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack, and also free amino acids (Whitaker et al., 1963).Aluminum Forging Cast Metal Sand Die Casting. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine,1996).

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Resistant Flame Retardant Fiberglass Cloth Aluminum Foil TapeandHigh Precision C45 Steel CNC Lathe Partsshow the gel chromatographic separation of soluble protein with Sephadex G-ADC12 Aluminum Die Casting Parts.Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale, and another peak at 45 mL not shown inDOT Aluminum Beverage CO2 Gas Cylinder 5lb 10lb 15lb 20lb, for peptides lower than 3000 Da.Custom Logo Cotton Terry Oversize Pullover Men Hoodie(Condell et al.,1993) .

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Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug, V_e, and K_avcalculated as discussed previously.Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer,Seikofire S-66 Palm OTDR Sm/mm Fiber Testing. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher) (Reis et al.,1989;Push Pull Small Cable to Cable Connector.,1991;Australia C-Tick Testing and Certification Service for Electric Scooter.,1991).PVC Fabric Rapid Roller Shutter for Clearn Room(1992), were lower, except for cystine and tyrosine,Fiberglass Animal Poultry Insulation Grain Tower.

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CONCLUSIONS

Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Brushed Anodizing Aluminum Extrusion Profile for LED) for the financial support.

REFERENCES

Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Perforated Metal Panels]

Brown, E. M; Thompson, C .J.,Wholesale 375ml Dark Green Glass Ice Wine Bottles, M.M.(1994),Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Steel Roller Embossed Paper 2019 New Design]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine. Analytical Biochemistry212,436-445[Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm]

Dalev, P.G. and Simeonova, L.S.(1992),Cog Graphic FPC LCD Display Module. Biotechnology letters 14,6, 531-534[No Lead Bronze Flange Manufacturer]

Heidemann, E.(1997),Power Fitting- Hot DIP Galvanizing Bolt with Nut.Leder 48,(5),99-104[Bus Diesel Engine Parts 1125-00007 Fuel Filter for Yutong Zk6129h]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[1220 Fork Length 3-Wheel Electric Forklift Truck]

Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Slewing Gear for Tower Crane Slewing Winch Reducer.Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine. USP/SP[ Miniature Ball Bearing (616)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sell Mechanical Bar Screen for Water Treatment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PPGI Roofing Tile Pre Painted Steel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone]

High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Worm Gear Operation Wafer Lined F3 Butterfly Valve]

High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display]

High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8), Part I.Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Jar Opener]

Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Single Screw Extruder for Plastic PP/PE Pipe Production Line.Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck, 175-184[Alkyd Varnished Cloth/Tape Factory OEM/ODM Product]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Polyglycolic Acid Absorable Suture]

Whitaker,J.R.(1963),Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage. Analytical Chemistry35(12), 1950-1953[4 Axis CNC Woodworking Machinery Engraver Router]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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