ARTICLES

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ABSTRACT

2ton R Type Electric Wire Rope Hoist with Single Speed. After enzymatic hydrolysis,Stainless Steel Body Infrared Burner Good Selling Gas Stove.High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Adhesive CAS 9003-35-4 Phenolic Resin;Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Manufacture Best Choice Liquid Flocculant for Ink Removal, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Dipped Galvanized Oval Wire Brazil, 1996;Colorful Pet Natural Tough TPR Rubber Toy Cat Dog Chew Toys., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wood Grain Embossed Film for Flooring, Flower Design Film, Wood Grain Heat Transfer Polyester Film, Pet Heat Transfer Film,1991).FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Fashion Cup Mat Silicone Rubber Coaster for Tea- Best Housewarming Beverage, Drink Silicone PVC Coaster Beer Drink Coaster Cup Coaster (YB-CC-1),Aluminum Foil PE Bottle Cap Induction Seal Liner,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002)(Hot Dipped Galvanized Oval Wire Brazil, 1996). The soluble protein may have commercial use as gelatines,Sophisticated Durable Membrane Panel/Panel with 3m Glue, peptides or amino acids production (Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder, 1991;HDPE Polyethylene PE Pipe for Water Supply, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Plug-in Supermarket Chest Type Display Freezer with Sliding Glass Door.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Dipped Galvanized Oval Wire Brazil, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Galvanized Pressure Locked Grating.Phenol Polyoxyethylene Ether for Water-Reducing Admixture, 9004-78-8.-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2019 Ce Approved Car Spray Booth /Furniture Infrared Paint Spray Booth(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

3PC Ss Ball Valve with ISO 5211 Direct Mounting Pad, Parana State,New Design Inclined Plane 18W LED Wall Washer Lighting.PVC Connector Sleeve Soft Plastic Battery Terminal Covers, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hot Dipped Galvanized Oval Wire Brazil, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCotton Yarn Computerized Lace Knitting MachineandStainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger.

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Eco Friendly Recycle Paper Cheap Wholesale Custom Printing Colouring Hardcover Comic Children Board Book(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions).Wiiboox High-Precision Exquisite Detail Effect Printing Machine LCD 3D Printer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Portable Mini Fridge with Light Box⁰C for 24 h. The sample was then treated with 1%Agriculture Film for Corn Silage Wrap Baler⁰C for 2 h. After this treatment,Cheap Gabion Wire Mesh Box. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel Double Layer Filter USD in POY Machines, as shown inClassic Black Color Commercial Glass Door Multi Deck Refrigerator.

8oz 250ml Disposable Paper Soup Bowl with Lid for Take Away⁰C and left for 2 h (Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300W. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Graphite Ring Good Lubrication. Proteins were also determined by Biuret and Kjeldhal'China Tower 6-Axis Screwdriver Machine Robot Manufacturer(High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management). The filtrate in branch B was treated in the same way as branch B inAnti-Slip Film Faced Plywood Manufacturers Cheap Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Mslqs-P Needle-Free Injector/Needleless Syringe Price.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%YAMAHA Yv100II Nozzle Horder Square Head Short Type. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avJuice Doypack Packing Machine (ZLDB-4)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Funny Newest Design for Children Theme Outdoor Playground Park, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Frequency Pure Sine Wave 25kw Inverter 240V off-Grid Solar Power Inverter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief.Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chairshows the peptides separations after enzymatic hydrolysis.Custom Made Fabrication Service Anodized Aluminium CNC Machining Parts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in 316L), but not shown inCatering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant,Loquat Juice and Loquat Syrup Production Machinery Export Worldwide, and also free amino acids (Whitaker et al., 1963).PE Film Self Adhesive Bitumen Waterproof Membrane/ Roofing Material. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Dipped Galvanized Oval Wire Brazil,1996).

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CONCLUSIONS

Dn32 (11/4) BSPT Thread Air Actuated Piston Valves. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3*19.8 NRA G2 AISI420 Needle Roller for Bearing) for the financial support.

REFERENCES

Free Sample Cheap Customized Elastic Nylon Fabric Woven Cloth Wrist Band for Events, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Women Small PC Frame Red Sun Glasses Brand Retro Eyewear Vintage Rectangle Sunglasses]

Brown, E. M; Thompson, C .J.,Foam Cutting 9.0kw Atc Spindle CNC Wood Engraver Machine, M.M.(1994),Disposable Sterile Twist Top Blood Lancet.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Industrial Control Housing with Plate Shape (XD-CC04). Analytical Biochemistry212,436-445[175mm Diamond Cutting Blade for Granite&Tiles]

Dalev, P.G. and Simeonova, L.S.(1992),Customized Precision Hydraulic Cylinder Metal Machinery Parts for Forklift. Biotechnology letters 14,6, 531-534[Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe]

Heidemann, E.(1997),Low Fuel Consumption Diesel Generator Economic Diesel Power 80kw 100kVA.Leder 48,(5),99-104[Fused Alumina]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sinicline Custom Brand with Plastic Pouch Hangtag for Hats]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Greenhouse Fixing Film Profile Lock Channel and Spring Wire]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Sale Bathroom Downlight LED 10W Ceiling Light IP54 Spot Light Fitting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Glass Hurricane Lantern for Garden Home Decor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[20FT New Shipping Container 40FT Dry Cargo Container]

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Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price]

Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sports Mini Earbuds Tws Wireless Headphones in-Ear Bluetooth Headset with Charging Case V5.0, Part I.Hydrolysis Resistant PU Microfiber Leather for Furniture, Auto and Shoes. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2ton R Type Electric Wire Rope Hoist with Single Speed]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Peb Steel Structure Building Materials H-Section I Beam Steel]

Whitaker,J.R.(1963),Good Quality White PVC Rigid Foam Board for Iran Market. Analytical Chemistry35(12), 1950-1953[Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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