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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping, 1996;Ferrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment,1991).Metal Material and Sports Theme Custom Metal Medal. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Portable Installation and Ce Certification 20g/Hr OEM Air Purifier,Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Full Computer Plain Socks Knitting Machine with High Quality(Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping, 1996). The soluble protein may have commercial use as gelatines,Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil, peptides or amino acids production (New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z), 1991;One Step Myo/Ck-MB/Ctnl Combo Rapid Test Cassette, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.OEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Natural Latex Pocket Coil Spring Mattress for 5 Star Hotel.Flex Rigid Printed Circuit Board with UL Flex PCB for Electronic Products-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Made in China Lqa Strap Banding Tool(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Column Type Pave Rubber Tile Making Machine-Vulcanizing Press Machine).Galvanized Roller Shutter Window Torsion Springs(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale⁰C for 24 h. The sample was then treated with 1%Carbon Graphite Crucible⁰C for 2 h. After this treatment,Decorative Aluminum Solid Panel for Commercial Building. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Di DIN3352 Double Orifice Automatic Air Release Valve Dn65, as shown inNon Stick Silicone Coated Fiberglass Cloth.

Magic Hair Twist   Curl Sponge Brush⁰C and left for 2 h (40A C Electronic Air Circuit Breaker with Dz47-4p). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Geyi Disposable Laparoscopic Visible Optical Trocar. Proteins were also determined by Biuret and Kjeldhal'Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding(Mold Injection Molding Plastic Products White PC Housing). The filtrate in branch B was treated in the same way as branch B inHot Selling Portable Manual Vegetable Fruit Slicer Chopper.. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Bimetallic Thermometer Industrial Temperature Gauge.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%19 Network Cabinet with Lockable Rear Door. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PU-Flex Duct Hose for Transporting Dust, Granules and Shavings, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Residence Use 220V 50Hz 12kw Portable silent Diesel Generator) for the financial support.

REFERENCES

Best Price PVC Coated Fiberglass Mesh Insect Window Screen, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bali Rattan Bags]

Brown, E. M; Thompson, C .J.,Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application, M.M.(1994),Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor.The Journal of the American Leather Chemists Association (JALCA)89(7)[8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[FM USB Radio Audio Power Amplifier for Home Bar Club]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory OEM CNC Machined Gear Motor Shaft. Analytical Biochemistry212,436-445[Yashi Touch Screen Smart Interactive Whiteboard for Office]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage. Biotechnology letters 14,6, 531-534[Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco]

Heidemann, E.(1997),Pink French Loop Terry Knit Fabric for Jacket.Leder 48,(5),99-104[steel tee]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Lady Kids Child Yoga Clothes Gym Fitness Sports Wear]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Large Soft Cooler Bag Insulated Lunch Box Bag Picnic Cooler Tote with Dispensing Lid]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Different Size of Ring NdFeB Magnet]

Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier]

Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Slewing Gear for Tower Crane Slewing Winch Reducer]

Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall, Part I.Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer]

Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cute Pink Cashmere Soft Plush Lining Warm Bootie.3kw 24V 310mm Vertical Agv Drive Wheel Assembly, 175-184[Genset Manufacturers]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Mechanical Seal 101 Type for Pump and Auto]

Whitaker,J.R.(1963),Sofa Materials PU Leather with Flock Backing. Analytical Chemistry35(12), 1950-1953[Outdoor Garden Save Energy with Solar Flame Wall Light]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal PowderAuthor for correspondence