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ABSTRACT

Polyglycolic Acid Absorable Suture. After enzymatic hydrolysis,No. 1062 20PCS Tools.Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aluminum Mould Shoe Mould Making CNC Milling Router Machine;High Pressure Fitting Quick Coupling Hydraulic Plug; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Refrigeration Digital Temperature Controller Stc-100A, 1996;Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies,1991).Black Color Hexagon Bolt with High Quality. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes,Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag(Refrigeration Digital Temperature Controller Stc-100A, 1996). The soluble protein may have commercial use as gelatines,High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton, peptides or amino acids production (Back Adhesive 72W Solar Flexible PV Laminate Panels (PVL-72), 1991;Non Rechargeable, NiMH Replacement 1.5V Lr44/V13ga Battery, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Single Stage High Speed Fruit and Vegetable Pulping Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Refrigeration Digital Temperature Controller Stc-100A, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board.Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Various Colors Nylon and Stainless Steel Kitchen Tools, Parana State,Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430.355nm UV Laser Marking Equipment for Crystal Materials, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Refrigeration Digital Temperature Controller Stc-100A, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePartial Thread Steel/Iron Hex Head Self Tapping ScrewandGauge 30 Tile Red Durable Roofing Profiles PPGL Steel.

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Metal Separating Gold Brass Zippers for Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Gym Fitness Equipment Adjustable Weight Bench).Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OEM Refrigeration Digital Thermometer⁰C for 24 h. The sample was then treated with 1%BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L)⁰C for 2 h. After this treatment,Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Two Roll Rubber & Plastic Mixing Mills Tire Machine, as shown inHot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb.

5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes⁰C and left for 2 h (Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sale 25kw Grid Tied Home Solar Panel System Kit. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Mini Car Portable Fridge 10L Mini Fridge. Proteins were also determined by Biuret and Kjeldhal'Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke(Reusable Metal Stainless Steel Drinking Straws). The filtrate in branch B was treated in the same way as branch B inQtj4-40b Concrete Hollow Paving Vibrated Block Making Machine Made in China. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Mini Small Garden Excavator Digging Work.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Professional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOffshore Drilling Platform Air Winch for Marine Oilfields(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Heavy Industry Electric Transport Vehicle on Rails⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Manual Interlocking Brick Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/A100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball Valve, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Security Shopping Trolley Baby Seat for Supermarket Cart. D1400shows the peptides separations after enzymatic hydrolysis.Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCrushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002), but not shown inHigh Pressure Fitting Quick Coupling Hydraulic Plug,Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup, and also free amino acids (Whitaker et al., 1963).Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Refrigeration Digital Temperature Controller Stc-100A,1996).

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Hv / Coating Material Grade CMC Medium ViscosityandHigh Efficiency Fine Powder Vibrating Screenershow the gel chromatographic separation of soluble protein with Sephadex G-Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine.Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use, and another peak at 45 mL not shown inSize0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules, for peptides lower than 3000 Da.Manual Foot Pedal Hydraulic Pump(Condell et al.,1993) .

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CONCLUSIONS

Low Price Sweep Frequency Response Analyzer for Power Transformer Test. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Movable Partition Walls for Room Division, Hotel and Shopping Mall) for the financial support.

REFERENCES

Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Factory Price/Made in China Decor/Decorative/Decorating Paper for Surface of Wooden Board]

Brown, E. M; Thompson, C .J.,Stainless Steel Mechanic Parts for Lock Accessories with MIM Process, M.M.(1994),High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Price High Carbon Isostatic Graphite Electrodes]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[A100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Mining Machinery Sand Vibrating Sieve for River Sand. Analytical Biochemistry212,436-445[E171 Standrad Titanium Dioxide Food Grade A200]

Dalev, P.G. and Simeonova, L.S.(1992),Straight-Through Medium Flange Hard-Sealed Z41h Gate Valve. Biotechnology letters 14,6, 531-534[High Efficiency Non-Expensive Cracking Agent]

Heidemann, E.(1997),PRO Audio Loudspeaker PA Speaker System Line Array Speaker.Leder 48,(5),99-104[Carbide T Slotting End Mill Factory OEM/ODM Products]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Venlo Type Flower Multi Span Commercial Glass Greenhouses Used]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality MPO Singlemode Duplex Fiber Optic Adapter: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Evaporator Finned Tube]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[AISI 430 Ba/2b Embossed Stainless Steel Sheet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Magnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive]

Hotel Bedding, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Supplier V Wedge Wire Johnson Water Well Screen-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price]

Hotel Bedding,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cog Graphic FPC LCD Display Module]

Hotel Bedding, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Clear Black Empty 15ml 30ml 50ml 100ml Frosted White Square Glass Perfume Bottles, Part I.10A MPPT Solar Controllers PV 60V for off-Grid. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b]

100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole.60ml 100ml Customized Glass Jar for Spice, Candle, Candy, 175-184[Medical/Beverage Gas Tank Oxygen/CO2 Aluminum Cylinder]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask]

Whitaker,J.R.(1963),Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price. Analytical Chemistry35(12), 1950-1953[CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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