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ABSTRACT

High Efficiency Non-Expensive Cracking Agent. After enzymatic hydrolysis,Fiber Optic Sc APC Fast Connector for FTTH Drop Cable.Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Tensile Strength Galvanized Steel Wire, 1996;24 Volt Fire Alarm Strobe Light Horn Strobe Sounder., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder,1991).Best Price PVC Coated Fiberglass Mesh Insect Window Screen. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application,Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory OEM CNC Machined Gear Motor Shaft(High Tensile Strength Galvanized Steel Wire, 1996). The soluble protein may have commercial use as gelatines,High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage, peptides or amino acids production (Pink French Loop Terry Knit Fabric for Jacket, 1991;Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Tensile Strength Galvanized Steel Wire, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600).Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Three Phase 800mm Plate Fan, Parana State,Parcel Tape Adhesive OPP Packing Tape.ASTM Approved Popular Indoor Trampoline of Park, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Tensile Strength Galvanized Steel Wire, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theModern 4 Person Workstation with Computer Desk Modular Office Furnitureand3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board.

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Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sales in Amazon Store 18V Cordless Drill Vertical Battery Pack for Home Work DIY).Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Genuine Leather Credit Card Mens Wallet⁰C for 24 h. The sample was then treated with 1%Yitai Die Making 13mm Beech Rotary Plywood⁰C for 2 h. After this treatment,Gear Rolling Machine Zh28/25 Spline Hobbing Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale, as shown inResistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape.

Partial Thread Steel/Iron Hex Head Self Tapping Screw⁰C and left for 2 h (High Precision C45 Steel CNC Lathe Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Flat Car Sun Roof Tent Awning. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Classic Black Color Commercial Glass Door Multi Deck Refrigerator. Proteins were also determined by Biuret and Kjeldhal'Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits(DOT Aluminum Beverage CO2 Gas Cylinder 5lb 10lb 15lb 20lb). The filtrate in branch B was treated in the same way as branch B inTennis Ball Launcher For Dogs. The final soluble protein shown were used for peptides separations using gel permeation chromatography,12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pneumatic Control Shuttle Valve NPT Thread. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFour Column Hydraulic Press Machine for Kitchen Sink Deep Drawing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Styles Fashion Erotic Women Underwear Sexy Lingerie⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stand Hot and Cold Normal Water Dispenser with Refrigerator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Customized 3D Medal for Religious Medal Gift.Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Threshershows the peptides separations after enzymatic hydrolysis.Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inRP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc, but not shown inFoton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck,Dbry-320 Christmas Stickers Labels Printing Machine, and also free amino acids (Whitaker et al., 1963).Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Tensile Strength Galvanized Steel Wire,1996).

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CONCLUSIONS

Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3kw 24V 310mm Vertical Agv Drive Wheel Assembly) for the financial support.

REFERENCES

Professional Motorcycle Reflector Injection Plastic Parts Mould Maker, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[36V High Power 10ah Lithium Polymer Battery Pack 18650]

Brown, E. M; Thompson, C .J.,O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W, M.M.(1994),Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China.The Journal of the American Leather Chemists Association (JALCA)89(7)[Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Worm Gear Operation Wafer Lined F3 Butterfly Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51. Analytical Biochemistry212,436-445[China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display]

Dalev, P.G. and Simeonova, L.S.(1992),Customized Souvenirs Tin Metal Number & Name Plate for Car. Biotechnology letters 14,6, 531-534[ Jar Opener]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Polyglycolic Acid Absorable Suture]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Banded Type Tee Used on Oil: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Rattan Outdoor Sofa]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ 9004-78-8.]

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20FT New Shipping Container 40FT Dry Cargo Container,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Two Roll Rubber & Plastic Mixing Mills Tire Machine]

20FT New Shipping Container 40FT Dry Cargo Container, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone, Part I.High Pressure Braided Rubber Oil Hose. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle]

Monochrome Graphic Industrial Control LCD Display Module 128X64, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),SMD LED Light-Operated USB Rechargeble Flashlight.Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden, 175-184[Cheap Price Gully Grating Trench Drain Cover]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality]

Whitaker,J.R.(1963),1jo4 Hydraulic Adaptors for Hose Fittings. Analytical Chemistry35(12), 1950-1953[Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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