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ABSTRACT

34*25cm Office File Pocket Felt A4 Document Bag. After enzymatic hydrolysis,PP Needlepunched Nonwoven Geotextile.High Temperaturethermocouple Male and Female Plug. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts;Best Price Custom Embroidery Logo Hotel Slippers; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Efficiency Fine Powder Vibrating Screener, 1996;High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plant Stands & Accessories Glass Hanging Flower Pot Bracket,1991).Wooden Frame Stretched Canvas, Painting Canvas. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine,SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,UF Membrane Aqu160 with Competitive Price and High Quality(High Efficiency Fine Powder Vibrating Screener, 1996). The soluble protein may have commercial use as gelatines,Atf-805 Auto Car Transmission System Flushing Machine, peptides or amino acids production (43 Glass Beads Cat Eye Aluminum Road Stud, 1991;Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Precision C45 Steel CNC Lathe Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Efficiency Fine Powder Vibrating Screener, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1.Food Grade PTFE Coated Fiberglass Mesh Baking Sheet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Beverage Cooling Cube Ice Making/Ice Maker Machinery(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Electrical Barber Shop Equipment Electric Hair Curling Wand Iron, Parana State,AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture.Really Microfiber PVC Leather for Sofa Furniture, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Efficiency Fine Powder Vibrating Screener, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theScreen Printing Materials and Equipments Screen Printing FramesandWomen Fashion Winter V Neck Long Sleeve Clothes.

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94V0 Circuit Board Mobile Phone PCB Motherboard(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Vinyl Glove in Work & Labor Glove).Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve⁰C for 24 h. The sample was then treated with 1%Adhesive Powder for Skim Coat/Wall Putty HPMC⁰C for 2 h. After this treatment,4X4 Galvanized Welded Wire Mesh Panel (FACTORY). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cotton Yarn Computerized Lace Knitting Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Plastic Caps Making Machine / Injection Molding Machine, as shown in700 mm RP HP UHP Graphite Electrode Sold at Factory Price.

Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate⁰C and left for 2 h (Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness. Proteins were also determined by Biuret and Kjeldhal'LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light(Movable Partition Walls for Room Division). The filtrate in branch B was treated in the same way as branch B in2 Wheels Electric Scooter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Safest Baby Healthcare and Grooming Kit for Baby Gift.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.1.6 Ton Hand Lifting Tool Lever Chain Block⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CNC Machining Service High Precision CNC Mechanical Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Home and Hospital Electric Patient LiftandVs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker. Enzymatic (Printed 100% Polyester Tcheap Wholesale Blankets)Food Grade Material 304/316 Double Diaphragm Pumps(sulfuric acid)Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels(High Efficiency Fine Powder Vibrating Screener, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125) for the financial support.

REFERENCES

8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Customized Garment Aluminum or Plastic Seal Tag]

Brown, E. M; Thompson, C .J.,Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9, M.M.(1994),Powder/Spices Powder Packing Machine with Four/Three Side Seal.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wood Veneer Peeling and Clipping Combined Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China PAC Water Flocculating Agent for Water Treatment]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Black Phosphated Bugle Head Csk Head Drywall Screw. Analytical Biochemistry212,436-445[Picosecond Laser Tattoo Removal Skin Care Medical Instrument]

Dalev, P.G. and Simeonova, L.S.(1992),Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine. Biotechnology letters 14,6, 531-534[ Machine for Making]

Heidemann, E.(1997),China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump.Leder 48,(5),99-104[Luxury Dining Table]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card]

Picc Puncture Care Kit, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence.6 Inch Plastic Breathing Cap for The IBC Valve. USP/SP[Steam Eye Mask Heating One Eye Mask for Sleeping]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Banded Type Tee Used on Oil]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aiyos Information Advertising Machine LCD Touch Screen Display]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building]

Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pet Anti-Static Needled Mat]

Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Black Small Fridge for Showcase]

Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part, Part I.Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Tall/Large Cube Glass Terrarium Planter Gift Card Holder]

Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer.Freshwater Pearl Butterfly Flower Bride Hairpin, 175-184[Rg58 BNC Male to BNC Female Connector Adapter Coaxial RF Pigtail Extension Cable]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[200W UFO LED High Bay Light Fixture for Interior Exterior Applications]

Whitaker,J.R.(1963),Air Bubble Insulation Thermal Building Insulation Material. Analytical Chemistry35(12), 1950-1953[Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Beverage Cooling Cube Ice Making/Ice Maker MachineryAuthor for correspondence