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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-DIN 7337 Aluminium Core Pulling Stainless Steel Rivet. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Professional Bb Trumpet (ATR6506)

ABSTRACT

Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines. After enzymatic hydrolysis,3.5ton Chinese Engine Gasoline Forklift (FG35T).Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle;Waterproof Covering Material Interior Wall Composite Panel; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Tall/Large Cube Glass Terrarium Planter Gift Card Holder, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Venlo Type Flower Multi Span Commercial Glass Greenhouses Used, 1996;XPS Extruded Polystyrene Foam Board., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture,1991).Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Kh No Fried Automatic Potato Chips Making Machines,Deep Hole Cylinder Boring Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Good Prize Solar PV Mounting Panel System for Ground(Venlo Type Flower Multi Span Commercial Glass Greenhouses Used, 1996). The soluble protein may have commercial use as gelatines,Electric Heavy Duty Rail Guided Vehicle for Transportation, peptides or amino acids production (Sower Vacuum Emulsifying Machine for Cosmetic, 1991;ASTM A240 309s Stainless Steel Sheet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Car Mattress Back Inflatable Air Bed Car.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Venlo Type Flower Multi Span Commercial Glass Greenhouses Used, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine.Single Embossed Buttons Membrane Switch with FPC Circuit-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container, Parana State,Professional Bb Trumpet (ATR6506).Chemicals Flocculant Cationic Polymer Price, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Venlo Type Flower Multi Span Commercial Glass Greenhouses Used, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBamboo Tablet Stand Laptop Rack Bt-2212andSpecial Flower Shaped Sterling Silver Cubic Zirconia Earring for Lady Wedding Gift.

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Ycom USB Charger and Data Cable with Aluminum for Type C(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Surprise! Range Hood Free! ! ! Juice Cart).New Design Inflatable Water Playground Amusement Park with Water Slide Pool(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface⁰C for 24 h. The sample was then treated with 1%Light Weight Polyester Eye Mask for Travel Airplane Hotel⁰C for 2 h. After this treatment,6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bamboo Tablet Stand Laptop Rack Bt-2212, as shown inCustomized Fashion Luxury Wooden Cosmetic Store Fixture for Shop.

Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book⁰C and left for 2 h (Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Personal Care Glutathione Skin Whitening Tablets. Proteins were also determined by Biuret and Kjeldhal'Customized Over Size Boys Hoodie with Embroidered( Foldable Esg10310). The filtrate in branch B was treated in the same way as branch B inSteel Toe Leather Protective Shoes. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Digital Printing Football Fans Flag.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFlb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.500 Tons Hydraulic Power Double Action Press with Cushion⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Promotional Plastic Laminated Custom Shopping PP Woven Bag, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring.Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Watershows the peptides separations after enzymatic hydrolysis.48V25A Lead Acid Battery Maintenance Pulse Charger(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in2+1 Flavors Soft Ice Cream Machine with Pre-Cooling, but not shown inStainless Steel Filter Strainer,15mm Best Price Commercial Grade Birch Plywood for Furniture, and also free amino acids (Whitaker et al., 1963).Top Great Wallet Genuine Leather Purse with Coin Pocket. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Venlo Type Flower Multi Span Commercial Glass Greenhouses Used,1996).

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CONCLUSIONS

Stainless Steel Clarifier Tank for Water Treatment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions) for the financial support.

REFERENCES

Carrier Roller for Sany Excavator, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Carbon Graphite Block Graphite Bar]

Brown, E. M; Thompson, C .J.,LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes, M.M.(1994),Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Mechanic Parts for Lock Accessories with MIM Process]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality MPO Singlemode Duplex Fiber Optic Adapter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts. Analytical Biochemistry212,436-445[Galvanized Solar System Mounting Bracket Connector]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets. Biotechnology letters 14,6, 531-534[China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes]

Heidemann, E.(1997),Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002).Leder 48,(5),99-104[m.kt-eyewear.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hanwha SMT Chip Shooter LED Pick and Place Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mining Machinery Sand Vibrating Sieve for River Sand]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Printed Heavy Duty Supplier 50kg Fertilizer PP Woven Bag Sack]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[S235jo S235jr Steel Pipe Tube Shs Rhs]

Dry Air Generator GF Series, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Microwave Motion Sensor Module Doppler Radar Detector (HW-M10)]

Dry Air Generator GF Series,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats]

Dry Air Generator GF Series, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Advanced Unlocking Tool for Locksmith, Part I.Screen Printing Materials and Equipments Screen Printing Frames. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate]

China Supplier Hard Ware Fittings Gas Spring for Tool Box, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price.Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed, 175-184[2016 OEM 2ml 9-425 HPLC Vial with Screw Cap and PTFE Septa Used for Lab Analysis Chromatography Sample Vial 100PCS/Pack]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026)]

Whitaker,J.R.(1963),New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet. Analytical Chemistry35(12), 1950-1953[Agriculture Film for Corn Silage Wrap Baler]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Insulating Thermal Insulation Silicone Rubber Coated Fiberglass ClothAuthor for correspondence